263 results found
Cooper JM, Cass AEG, 2004, Biosensors: a practical approach, Publisher: Oxford University Press, ISBN: 9780199638451
Wada A, Mie M, Aizawa M, et al., 2003, Design and construction of glutamine binding proteins with a self-adhering capability to unmodified hydrophobic surfaces as reagentless fluorescence sensing devices, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Vol: 125, Pages: 16228-16234, ISSN: 0002-7863
Noble JE, Ganju P, Cass AEG, 2003, Fluorescent peptide probes for high-throughput measurement of protein phosphatases, ANALYTICAL CHEMISTRY, Vol: 75, Pages: 2042-2047, ISSN: 0003-2700
Cass A, Cunningham J, Snelling P, et al., 2003, Renal transplantation for Indigenous Australians: identifying the barriers to equitable access., Ethn Health, Vol: 8, Pages: 111-119, ISSN: 1355-7858
OBJECTIVE: To assess Indigenous Australians' access to renal transplantation, compared with non-Indigenous Australians. To examine whether disparities are due to a lower rate of acceptance onto the waiting list and/or a lower rate of moving from the list to transplantation. DESIGN: National cohort study using data from the Australian and New Zealand Dialysis and Transplant Registry. We included all end-stage renal disease (ESRD) patients under 65 years of age who started treatment in Australia between January 1993 and December 1998. We used survival analysis to examine the time from commencement of renal replacement therapy (RRT) to transplantation. We measured time from commencement of RRT to acceptance onto the waiting list (stage 1), and time from acceptance onto the waiting list to transplantation (stage 2). The main outcome measures were (1) acceptance onto the waiting list and (2) receipt of a transplant, before 31 March 2000. RESULTS: Indigenous patients had a lower transplantation rate (adjusted Indigenous: non-Indigenous rate ratio 0.32, 95% CI 0.25-0.40). They had both a lower rate of acceptance onto the waiting list (adjusted rate ratio 0.50, 95% CI 0.44-0.57) and a lower rate of moving from the list to transplantation (adjusted rate ratio 0.50, 95% CI 0.38-0.65). The disparities were not explained by differences in age, sex, co-morbidities or cause of renal disease. CONCLUSIONS: Indigenous Australians face barriers to acceptance onto the waiting list and to moving from the list to transplantation. Further research to identify the causes could facilitate strategies to improve equity in transplantation.
Xu HF, Zhang XE, Zhang ZP, et al., 2003, The in vitro directed evolution of E. coli alkaline phosphatase, Progress in Biochemistry and Biophysics, Vol: 30, Pages: 89-94, ISSN: 1000-3282
The evolution of phoA gene fragment distant from the Asp101-Ser102-Ala103 encoding region to increase the catalytic activity of EAP with a single mutant D101S as parent was directed. Through two cycles of error prone PCR, coupled with a sensitive screening method, an evolved variant 4-186 was obtained. Its catalytic activity was 3-fold higher than that of D101S parent and 35-fold more active than wild-type EAP. The kinetic analysis indicated that the evolved enzyme exhibits a higher substrate binding ability and a higher catalytic efficiency than the D101S parent enzyme. DNA sequence revealed that 4-186 contains two amino acid substitutions, K167R and S374C, both of which locate neither the substrate-binding sites nor the metal-binding sites of EAP.
Xu HF, Zhang XE, Zhang ZP, et al., 2003, Directed evolution of E-coli alkaline phosphatase towards higher catalytic activity, BIOCATALYSIS AND BIOTRANSFORMATION, Vol: 21, Pages: 41-47, ISSN: 1024-2422
Simon E, Halliwell CM, Toh CS, et al., 2002, Oxidation of NADH produced by a lactate dehydrogenase immobilised on poly(aniline)-poly(anion) composite films, JOURNAL OF ELECTROANALYTICAL CHEMISTRY, Vol: 538, Pages: 253-259, ISSN: 0022-0728
Halliwell CM, Simon E, Toh CS, et al., 2002, A method for the determination of enzyme mass loading on an electrode surface through radioisotope labelling, BIOSENSORS & BIOELECTRONICS, Vol: 17, Pages: 965-972, ISSN: 0956-5663
Chen LQ, Zhang XE, Xie WH, et al., 2002, Genetic modification of glucose oxidase for improving performance of an amperometric glucose biosensor, BIOSENSORS & BIOELECTRONICS, Vol: 17, Pages: 851-857, ISSN: 0956-5663
Bi L-J, Zhou Y-F, Deng J-Y, et al., 2002, [High expression and identification of DNA mismatch repair gene mutS in Escherichia coli]., Sheng Wu Gong Cheng Xue Bao, Vol: 18, Pages: 536-540, ISSN: 1000-3061
DNA mismatch repair gene mutS (2.56 kb) was PCR modified and cloned into a secretive prokaryotic expression vector pET32a (+) which carries a N-terminal His.tag + and thioredoxin sequence. MutS protein was expressed with high level after IPTG induction using the strain E. coli AD494(DE3). SDS-PAGE revealed that the expected protein with a molecular weight of 108 kD which is about 35% of the total bacterial proteins is almost soluble. The expected protein was purified directly by immobilized metal (Ni2+) chelation affinity chromatography and the purity is over 90%. MutS protein activity verified using mismatch DNA showed that the expression product can recognize and bind to base-pair mismatch specifically.
Xu HF, Zhang XE, Zhang YM, et al., 2002, Recent advances in in vitro molecular directed evolution, PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS, Vol: 29, Pages: 518-522, ISSN: 1000-3282
Cooper JM, Cass AEG, 2002, Miniaturized screening technologies for drug discovery, y593kzg on High-Throughput Screening - The Way Ahead, Publisher: PORTLAND PRESS, Pages: 802-806, ISSN: 0300-5127
Zhou Y-F, Zhang X-E, Cass AEG, 2002, [Current researches on molecular enzyme engineering]., Sheng Wu Gong Cheng Xue Bao, Vol: 18, Pages: 401-406, ISSN: 1000-3061
The developments of recombinant DNA technology and structural biology make it possible to modify enzyme in molecular level. Scientists show growing interests in the evolution or functional fusion of enzymes. Recent advances and applications of the molecular enzyme engineering are reviewed and discussed in this article.
Halliwell CM, Simon E, Toh CS, et al., 2002, Immobilisation of lactate dehydrogenase on poly(aniline)-poly(acrylate) and poly(aniline)-poly-(vinyl sulphonate) films for use in a lactate biosensor, ANALYTICA CHIMICA ACTA, Vol: 453, Pages: 191-200, ISSN: 0003-2670
Cass T, Dunwell J, Wackett LP, et al., 2002, Biotechnology. Paper Alert., Curr Opin Biotechnol, Vol: 13, Pages: 1-8, ISSN: 0958-1669
A selection of interesting papers that were published in the two months before our press date in major journals most likely to report significant results in biotechnology.
Zhang XE, Zhou YH, Zhang ZP, et al., 2002, Engineering E-coli alkaline phosphatase yields changes of catalytic activity, thermal stability and phosphate inhibition, BIOCATALYSIS AND BIOTRANSFORMATION, Vol: 20, Pages: 381-389, ISSN: 1024-2422
Simon E, Halliwell CM, Toh CS, et al., 2002, Immobilisation of enzymes on poly(aniline)-poly(anion) composite films. Preparation of bioanodes for biofuel cell applications, 26th International Symposium on Bioelectrochemistry and Bioenergetics, Publisher: ELSEVIER SCIENCE SA, Pages: 13-15, ISSN: 1567-5394
Hudson J, Cass AEG, Prabhananda BS, 2002, Synergy in K+/H+ exchange across phospholipid vesicle membranes with combinations of valinomycin and chlorophenols, PHYSICAL CHEMISTRY CHEMICAL PHYSICS, Vol: 4, Pages: 2702-2707, ISSN: 1463-9076
Tsotsou GE, Cass AEG, Gilardi G, 2002, High throughput assay for cytochrome P450BM3 for screening libraries of substrates and combinatorial mutants, BIOSENSORS & BIOELECTRONICS, Vol: 17, Pages: 119-131, ISSN: 0956-5663
Halliwell CM, Simon E, Toh CS, et al., 2002, The design of dehydrogenase enzymes for use in a biofuel cell: the role of genetically introduced peptide tags in enzyme immobilization on electrodes, BIOELECTROCHEMISTRY, Vol: 55, Pages: 21-23, ISSN: 1567-5394
Cass AEG, 2002, Biosensors, Encyclopedia of Molecular Medicine, New York, Publisher: Wiley, Pages: 369-371, ISBN: 9780471374947
Topoglidis E, Cass AEG, O'Regan B, et al., 2001, Immobilisation and bioelectrochemistry of proteins on nanoporous TiO2 and ZnO films, JOURNAL OF ELECTROANALYTICAL CHEMISTRY, Vol: 517, Pages: 20-27, ISSN: 0022-0728
Topoglidis E, Campbell CJ, Cass AEG, et al., 2001, Factors that affect protein adsorption on nanostructured titania films. A novel spectroelectrochemical application to sensing, LANGMUIR, Vol: 17, Pages: 7899-7906, ISSN: 0743-7463
Zhou YF, Zhang XE, Liu H, et al., 2001, Construction of a fusion enzyme system by gene splicing as a new molecular recognition element for a sequence biosensor, BIOCONJUGATE CHEMISTRY, Vol: 12, Pages: 924-931, ISSN: 1043-1802
Cooper J, Disley D, Cass T, 2001, Microsystems special - Lab-on-a-chip and microarrays, CHEMISTRY & INDUSTRY, Pages: 653-655, ISSN: 0009-3068
Halliwell CM, Morgan G, Ou CP, et al., 2001, Introduction of a (poly)histidine tag in L-lactate dehydrogenase produces a mixture of active and inactive molecules, ANALYTICAL BIOCHEMISTRY, Vol: 295, Pages: 257-261, ISSN: 0003-2697
Zhou YF, Zhang XE, Liu H, et al., 2001, [Cloning and expression of Aspergillus niger glucose oxidase gene in methylotrophic yeast]., Sheng Wu Gong Cheng Xue Bao, Vol: 17, Pages: 400-405, ISSN: 1000-3061
The DNA fragment encoding A. niger glucose oxidase was amplified by PCR using A. niger genomic DNA as template, and was cloned into vector of pPIC9 for expression in Pichia pastoris. When transformed into methylotrophic yeast Pichia pastoris GS115, The constructed plasmid pPICGOD1 directed the synthesis and secretion of functionally active GOD. After induction in MM medium for 4 days, the GOD activity in the medium reached 30-40 u/mL. SDS-PAGE revealed that recombinant yeast GOD was expressed up to 60%-70% of the total soluble protein, and the secreted GOD could be purified to electrophoretic homogeneity with one purification step using Q Sepharose Fast Flow ion exchange chromatography. The recombinant yeast GOD had very high catalytic activity, showed about 1.6-fold increase of specific activity over the commercial A. niger GOD. Kinetic analysis clearly demonstrated that recombinant yeast GOD showed similar substrate affinity for glucose to A. niger GOD, but the turnover number of the GOD from yeast was determined to be much higher than that of A. niger GOD. In addition, the linear range of glucose electrode made with recombinant yeast GOD was efficiently widened due to the high catalytic activity of yeast GOD.
Halliwell CM, Cass AEG, 2001, A factorial analysis of silanization conditions for the immobilization of oligonucleotides on glass surfaces, ANALYTICAL CHEMISTRY, Vol: 73, Pages: 2476-2483, ISSN: 0003-2700
Zhang JK, Cass AEG, 2001, A study of his-tagged alkaline phosphatase immobilization on a nanoporous nickel-titanium dioxide film, ANALYTICAL BIOCHEMISTRY, Vol: 292, Pages: 307-310, ISSN: 0003-2697
Topoglidis E, Campbell CJ, Cass AEG, et al., 2001, Biosensors based on protein adsorption on nanoporous TiO2 films, Chemical and Biological sensors and analytical methods II, Editors: Butler, Vanysek, Yamazoe, Publisher: International Society of Electrochemistry, Pages: 196-202, ISBN: 9781566773515
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