Imperial College London

Professor Tony Cass

Faculty of Natural SciencesDepartment of Chemistry

Professor of Chemistry
 
 
 
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Contact

 

+44 (0)20 7594 5195t.cass

 
 
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Location

 

301KMolecular Sciences Research HubWhite City Campus

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Summary

 

Publications

Publication Type
Year
to

267 results found

Zhou YF, Zhang XE, Liu H, Zhang CG, Cass AEet al., 2001, [Cloning and expression of Aspergillus niger glucose oxidase gene in methylotrophic yeast]., Sheng Wu Gong Cheng Xue Bao, Vol: 17, Pages: 400-405, ISSN: 1000-3061

The DNA fragment encoding A. niger glucose oxidase was amplified by PCR using A. niger genomic DNA as template, and was cloned into vector of pPIC9 for expression in Pichia pastoris. When transformed into methylotrophic yeast Pichia pastoris GS115, The constructed plasmid pPICGOD1 directed the synthesis and secretion of functionally active GOD. After induction in MM medium for 4 days, the GOD activity in the medium reached 30-40 u/mL. SDS-PAGE revealed that recombinant yeast GOD was expressed up to 60%-70% of the total soluble protein, and the secreted GOD could be purified to electrophoretic homogeneity with one purification step using Q Sepharose Fast Flow ion exchange chromatography. The recombinant yeast GOD had very high catalytic activity, showed about 1.6-fold increase of specific activity over the commercial A. niger GOD. Kinetic analysis clearly demonstrated that recombinant yeast GOD showed similar substrate affinity for glucose to A. niger GOD, but the turnover number of the GOD from yeast was determined to be much higher than that of A. niger GOD. In addition, the linear range of glucose electrode made with recombinant yeast GOD was efficiently widened due to the high catalytic activity of yeast GOD.

Journal article

Halliwell CM, Cass AEG, 2001, A factorial analysis of silanization conditions for the immobilization of oligonucleotides on glass surfaces, ANALYTICAL CHEMISTRY, Vol: 73, Pages: 2476-2483, ISSN: 0003-2700

Journal article

Zhang JK, Cass AEG, 2001, A study of his-tagged alkaline phosphatase immobilization on a nanoporous nickel-titanium dioxide film, ANALYTICAL BIOCHEMISTRY, Vol: 292, Pages: 307-310, ISSN: 0003-2697

Journal article

Topoglidis E, Campbell CJ, Cass AEG, Durrant JRet al., 2001, Biosensors based on protein adsorption on nanoporous TiO2 films, Chemical and Biological sensors and analytical methods II, Editors: Butler, Vanysek, Yamazoe, Publisher: International Society of Electrochemistry, Pages: 196-202, ISBN: 9781566773515

Book chapter

Zhou YF, Zhang XE, Liu H, Zhang CG, Cass AEet al., 2001, Cloning and expression of aspergillus niger glucose oxidase gene in methylotrophic yeast, Chinese Journal of Biotechnology (Chinese Edition), Vol: 17, Pages: 405-408, ISSN: 1000-3061

Journal article

Cooper JM, Cass AEG, Yang H, 2001, Microfabricated biochip platforms for cell analyses, Biochip technology, Editors: Kricka, Cheng, Publisher: Harwood Academic, Pages: 309-324, ISBN: 9789057026133

Book chapter

Shao WH, Zhang XE, Liu H, Zhang ZPet al., 2000, Anchor-chain molecular system for orientation control in enzyme immobilization, BIOCONJUGATE CHEMISTRY, Vol: 11, Pages: 822-826, ISSN: 1043-1802

Journal article

Cass T, 2000, Prism, SCIENCE, Vol: 289, Pages: 1158-1158, ISSN: 0036-8075

Journal article

Zhang JK, Cass AEG, 2000, Electrochemical analysis of immobilised chemical and genetic biotinylated alkaline phosphatase, ANALYTICA CHIMICA ACTA, Vol: 408, Pages: 241-247, ISSN: 0003-2670

Journal article

Glidle A, Hadyoon CS, Cass AEG, Cooper JMet al., 2000, Modifications of electrodeposited polymers by ion chelation to produce templates for biomolecule immobilisation, ELECTROCHIMICA ACTA, Vol: 45, Pages: 3823-3831, ISSN: 0013-4686

Journal article

Topoglidis E, Lutz T, Willis RL, Barnett CJ, Cass AEG, Durrant JRet al., 2000, Protein adsorption on nanoporous TiO2 films: a novel approach to studying photoinduced protein/electrode transfer reactions, Faraday Discussions, Pages: 35-46, ISSN: 0301-7249

Journal article

Cass AEG, Valat C, Steel A, 2000, Immobilized Nucleic Acid Hybridization Reagent and Meth, WO042222A2

Patent

Cass T, 1999, ImagePro Plus, BIOTECHNOLOGY SOFTWARE & INTERNET JOURNAL, Vol: 16, Pages: 24-25, ISSN: 1088-4270

Journal article

Davis J, Glidle A, Cass AEG, Zhang JK, Cooper JMet al., 1999, Spectroscopic evaluation of protein affinity binding at polymeric biosensor films, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Vol: 121, Pages: 4302-4303, ISSN: 0002-7863

Journal article

Durrant JR, Cass AEG, Gilardi G, 1999, Biochemical Devices And Their Methods of Manufacture, WO9954718A1

Patent

Cass T, 1998, HiQ, SCIENCE, Vol: 282, Pages: 636-636, ISSN: 0036-8075

Journal article

Cass T, 1998, OLIGO 6.0, BIOTECHNOLOGY SOFTWARE & INTERNET JOURNAL, Vol: 15, Pages: 22-23, ISSN: 1088-4270

Journal article

Nagy JM, Jesmin J, Servos S, Cass AEG, Brown KAet al., 1998, Site-directed mutants of the catalase-peroxidase from Mycobacterium tuberculosis, BIOCHEMICAL SOCIETY TRANSACTIONS, Vol: 26, Pages: S281-S281, ISSN: 0300-5127

Journal article

Chen X, Zhang XE, Chai YQ, Hu WP, Zhang ZP, Zhang XM, Cass AEGet al., 1998, DNA optical sensor: a rapid method for the detection of DNA hybridization, BIOSENSORS & BIOELECTRONICS, Vol: 13, Pages: 451-458, ISSN: 0956-5663

Journal article

Cass AEG, Cass T, Ligler FS, 1998, Immobilized biomolecules in analysis, Publisher: Oxford University Press, USA, ISBN: 9780199636365

This book provides a selection of methods for the immobilization of biomolecules and cells on a variety of surface with different geometries and chemistriesso that they retain their function and guidelines on which method to use.

Book

Topoglidis E, Cass AEG, Gilardi G, Sadeghi S, Beaumont N, Durrant JRet al., 1998, Protein adsorption on nanocrystalline TiO2 films: An immobilization strategy for bioanalytical devices, Analytical Chemistry, Vol: 70, Pages: 5111-5113, ISSN: 0003-2700

Journal article

Sadeghi SJ, Gilardi G, Cass AEG, 1997, Mediated electrochemistry of peroxidases - effects of variations in protein and mediator structures, BIOSENSORS & BIOELECTRONICS, Vol: 12, Pages: 1191-1198, ISSN: 0956-5663

Journal article

Nagy JM, Cass AEG, Brown KA, 1997, Purification and characterization of recombinant catalase-peroxidase, which confers isoniazid sensitivity in Mycobacterium tuberculosis, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 272, Pages: 31265-31271, ISSN: 0021-9258

Journal article

Nagy JM, Svergun D, Koch MHJ, Cass AEG, Brown KAet al., 1997, Structural characterization of recombinant catalase-peroxidase from Mycobacterium tuberculosis, BIOCHEMICAL SOCIETY TRANSACTIONS, Vol: 25, Pages: S617-S617, ISSN: 0300-5127

Journal article

Antiochia R, Cass AEG, Palleschi G, 1997, Purification and sensor applications of an oxygen insensitive, thermophilic diaphorase, ANALYTICA CHIMICA ACTA, Vol: 345, Pages: 17-28, ISSN: 0003-2670

Journal article

Gilardi G, Mei G, Rosato N, Agro AF, Cass AEGet al., 1997, Spectroscopic properties of an engineered maltose binding protein, PROTEIN ENGINEERING, Vol: 10, Pages: 479-486, ISSN: 0269-2139

Journal article

Cass AEG, Gilardi G, Beaumont NJ, Sadeghi SJet al., 1997, Producing new sensing molecules with gene technology., ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, Vol: 213, Pages: 220-ANYL, ISSN: 0065-7727

Journal article

Sadeghi SJ, Gilardi G, Nicolosi G, Cass AEGet al., 1997, Chiral discrimination in the oxidation of ferrocenes by cytochrome c peroxidase, CHEMICAL COMMUNICATIONS, Pages: 517-518, ISSN: 1359-7345

Journal article

Hengsakul M, Cass AEG, 1997, Alkaline phosphatase-strep tag fusion protein binding to streptavidin: Resonant mirror studies, JOURNAL OF MOLECULAR BIOLOGY, Vol: 266, Pages: 621-632, ISSN: 0022-2836

Journal article

Nagy JM, Svergun D, Koch MHJ, Cass AEG, Brown KAet al., 1997, 82 Structural characterization of recombinant catalase-peroxidase from Mycobacterium tuberculosis, Biochemical Society Transactions, Vol: 25, ISSN: 0300-5127

Journal article

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