Imperial College London

Professor Tony Cass

Faculty of Natural SciencesDepartment of Chemistry

Professor of Chemistry
 
 
 
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Contact

 

+44 (0)20 7594 5195t.cass

 
 
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Location

 

301KMolecular Sciences Research HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Mie:2019:10.1007/s11033-018-4467-2,
author = {Mie, M and Matsumoto, R and Mashimo, Y and Cass, AEG and Kobatake, E},
doi = {10.1007/s11033-018-4467-2},
journal = {Molecular Biology Reports},
pages = {261--269},
title = {Development of drug-loaded protein nanoparticles displaying enzymatically-conjugated DNA aptamers for cancer cell targeting},
url = {http://dx.doi.org/10.1007/s11033-018-4467-2},
volume = {46},
year = {2019}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Modification of protein-based drug carriers with tumor-targeting properties is an important area of research in the field of anticancer drug delivery. To this end, we developed nanoparticles comprised of elastin-like polypeptides (ELPs) with fused poly-aspartic acid chains (ELP-D) displaying DNA aptamers. DNA aptamers were enzymatically conjugated to the surface of the nanoparticles via genetic incorporation of Gene A protein into the sequence of the ELP-D fusion protein. Gene A protein, derived from bacteriophage X174, can form covalent complexes with single-stranded DNA via the latter's recognition sequence. Gene A protein-displaying nanoparticles exhibited the ability to deliver the anticancer drug paclitaxel (PTX), whilst retaining activity of the conjugated Gene A protein. PTX-loaded protein nanoparticles displaying DNA aptamers known to bind to the MUC1 tumor marker resulted in increased cytotoxicity with MCF-7 breast cancer cells compared to PTX-loaded protein nanoparticles without the DNA aptamer modification.
AU - Mie,M
AU - Matsumoto,R
AU - Mashimo,Y
AU - Cass,AEG
AU - Kobatake,E
DO - 10.1007/s11033-018-4467-2
EP - 269
PY - 2019///
SN - 0301-4851
SP - 261
TI - Development of drug-loaded protein nanoparticles displaying enzymatically-conjugated DNA aptamers for cancer cell targeting
T2 - Molecular Biology Reports
UR - http://dx.doi.org/10.1007/s11033-018-4467-2
UR - https://www.ncbi.nlm.nih.gov/pubmed/30421127
UR - http://hdl.handle.net/10044/1/66185
VL - 46
ER -