Imperial College London

Professor Tony Cass

Faculty of Natural SciencesDepartment of Chemistry

Professor of Chemistry
 
 
 
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Contact

 

+44 (0)20 7594 5195t.cass

 
 
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Location

 

301KMolecular Sciences Research HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Liang:2019:10.1038/s41467-019-08799-6,
author = {Liang, S and Kinghorn, AB and Voliotis, M and Prague, JK and Veldhuis, JD and Tsaneva-Atanasova, K and McArdle, CA and Li, RHW and Cass, AEG and Dhillo, WS and Tanner, JA},
doi = {10.1038/s41467-019-08799-6},
journal = {Nature Communications},
title = {Measuring luteinising hormone pulsatility with a robotic aptamer-enabled electrochemical reader},
url = {http://dx.doi.org/10.1038/s41467-019-08799-6},
volume = {10},
year = {2019}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Normal reproductive functioning is critically dependent on pulsatile secretion of luteinising hormone (LH). Assessment of LH pulsatility is important for the clinical diagnosis of reproductive disorders, but current methods are hampered by frequent blood sampling coupled to expensive serial immunochemical analysis. Here, we report the development and application of a Robotic APTamer-enabled Electrochemical Reader (RAPTER) electrochemical analysis system to determine LH pulsatility. Through selective evolution of ligands by exponential enrichment (SELEX), we identify DNA aptamers that bind specifically to LH and not to related hormones. The aptamers are integrated into electrochemical aptamer-based (E-AB) sensors on a robotic platform. E-AB enables rapid, sensitive and repeatable determination of LH concentration profiles. Bayesian Spectrum Analysis is applied to determine LH pulsatility in three distinct patient cohorts. This technology has the potential to transform the clinical care of patients with reproductive disorders and could be developed to allow real-time in vivo hormone monitoring.
AU - Liang,S
AU - Kinghorn,AB
AU - Voliotis,M
AU - Prague,JK
AU - Veldhuis,JD
AU - Tsaneva-Atanasova,K
AU - McArdle,CA
AU - Li,RHW
AU - Cass,AEG
AU - Dhillo,WS
AU - Tanner,JA
DO - 10.1038/s41467-019-08799-6
PY - 2019///
SN - 2041-1723
TI - Measuring luteinising hormone pulsatility with a robotic aptamer-enabled electrochemical reader
T2 - Nature Communications
UR - http://dx.doi.org/10.1038/s41467-019-08799-6
UR - http://hdl.handle.net/10044/1/67289
VL - 10
ER -