Imperial College London

Professor Tony Cass

Faculty of Natural SciencesDepartment of Chemistry

Professor of Chemistry
 
 
 
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Contact

 

+44 (0)20 7594 5195t.cass

 
 
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Location

 

301KMolecular Sciences Research HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Sze:2017:10.1038/s41467-017-01584-3,
author = {Sze, JYY and Ivanov, AP and Cass, AEG and Edel, JB},
doi = {10.1038/s41467-017-01584-3},
journal = {Nature Communications},
title = {Single Molecule Multiplexed Nanopore Protein Screening in Human Serum using Aptamer modified DNA Carriers},
url = {http://dx.doi.org/10.1038/s41467-017-01584-3},
volume = {8},
year = {2017}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - The capability to screen a range of proteins at the single-molecule level with enhanced selectivity in biological fluids has been in part a driving force in developing future diagnostic and therapeutic strategies. The combination of nanopore sensing and nucleic acid aptamer recognition comes close to this ideal due to the ease of multiplexing, without the need for expensive labelling methods or extensive sample pre-treatment. Here, we demonstrate a fully flexible, scalable and low-cost detection platform to sense multiple protein targets simultaneously by grafting specific sequences along the backbone of a double-stranded DNA carrier. Protein bound to the aptamer produces unique ionic current signatures which facilitates accurate target recognition. This powerful approach allows us to differentiate individual protein sizes via characteristic changes in the sub-peak current. Furthermore, we show that by using DNA carriers it is possible to perform single-molecule screening in human serum at ultra-low protein concentrations.
AU - Sze,JYY
AU - Ivanov,AP
AU - Cass,AEG
AU - Edel,JB
DO - 10.1038/s41467-017-01584-3
PY - 2017///
SN - 2041-1723
TI - Single Molecule Multiplexed Nanopore Protein Screening in Human Serum using Aptamer modified DNA Carriers
T2 - Nature Communications
UR - http://dx.doi.org/10.1038/s41467-017-01584-3
UR - http://hdl.handle.net/10044/1/51671
VL - 8
ER -