262 results found
Gowers SAN, Freeman DME, Rawson TM, et al., 2019, Development of a Minimally Invasive Microneedle-Based Sensor for Continuous Monitoring of β-Lactam Antibiotic Concentrations in Vivo., ACS Sens, Vol: 4, Pages: 1072-1080
Antimicrobial resistance poses a global threat to patient health. Improving the use and effectiveness of antimicrobials is critical in addressing this issue. This includes optimizing the dose of antibiotic delivered to each individual. New sensing approaches that track antimicrobial concentration for each patient in real time could allow individualized drug dosing. This work presents a potentiometric microneedle-based biosensor to detect levels of β-lactam antibiotics in vivo in a healthy human volunteer. The biosensor is coated with a pH-sensitive iridium oxide layer, which detects changes in local pH as a result of β-lactam hydrolysis by β-lactamase immobilized on the electrode surface. Development and optimization of the biosensor coatings are presented, giving a limit of detection of 6.8 μM in 10 mM PBS solution. Biosensors were found to be stable for up to 2 weeks at -20 °C and to withstand sterilization. Sensitivity was retained after application for 6 h in vivo. Proof-of-concept results are presented showing that penicillin concentrations measured using the microneedle-based biosensor track those measured using both discrete blood and microdialysis sampling in vivo. These preliminary results show the potential of this microneedle-based biosensor to provide a minimally invasive means to measure real-time β-lactam concentrations in vivo, representing an important first step toward a closed-loop therapeutic drug monitoring system.
Sharma S, Howells O, Rajendran N, et al., 2019, Microneedle array-based platforms for future theranostic applications., Chembiochem
Theranostics involves finding the biomarkers of a disease, fighting them through site specific drug delivery and following them for prognosis of the disease. Microneedle array technology has been used for drug delivery and extended for continuous monitoring of analytes present in the skin compartment. We envisage the use of microneedle arrays for future theranostic applications. The potential of using combined microneedle array-based drug delivery and diagnostics as part of closed-loop control system for the management of diseases and delivery of precision drugs in individual patients, is reported in this paper.
Hansel CS, Crowder SW, Cooper S, et al., 2019, Nanoneedle-Mediated Stimulation of Cell Mechanotransduction Machinery, ACS NANO, Vol: 13, Pages: 2913-2926, ISSN: 1936-0851
Liang S, Kinghorn AB, Voliotis M, et al., 2019, Measuring luteinising hormone pulsatility with a robotic aptamer-enabled electrochemical reader, NATURE COMMUNICATIONS, Vol: 10, ISSN: 2041-1723
Mie M, Matsumoto R, Mashimo Y, et al., 2019, Development of drug-loaded protein nanoparticles displaying enzymatically-conjugated DNA aptamers for cancer cell targeting, MOLECULAR BIOLOGY REPORTS, Vol: 46, Pages: 261-269, ISSN: 0301-4851
Bollella P, Sharma S, Cass AEG, et al., 2019, Minimally-invasive Microneedle-based Biosensor Array for Simultaneous Lactate and Glucose Monitoring in Artificial Interstitial Fluid, ELECTROANALYSIS, Vol: 31, Pages: 374-382, ISSN: 1040-0397
Grell M, Dincer C, Le T, et al., 2019, Autocatalytic Metallization of Fabrics Using Si Ink, for Biosensors, Batteries and Energy Harvesting, ADVANCED FUNCTIONAL MATERIALS, Vol: 29, ISSN: 1616-301X
Bollella P, Sharma S, Cass AEG, et al., 2019, Microneedle-based biosensor for minimally-invasive lactate detection, BIOSENSORS & BIOELECTRONICS, Vol: 123, Pages: 152-159, ISSN: 0956-5663
Rawson TM, Ming D, Gowers SAN, et al., 2019, Public acceptability of computer-controlled antibiotic management: An exploration of automated dosing and opportunities for implementation, JOURNAL OF INFECTION, Vol: 78, Pages: 82-84, ISSN: 0163-4453
Maniyam MN, Ibrahim AL, Cass AEG, 2019, Enhanced cyanide biodegradation by immobilized crude extract of Rhodococcus UKMP-5M, ENVIRONMENTAL TECHNOLOGY, Vol: 40, Pages: 386-398, ISSN: 0959-3330
Singh M, Nabavi E, Zhou Y, et al., 2019, Laparoscopic fluorescence image-guided photothermal therapy enhances cancer diagnosis and treatment., Nanotheranostics, Vol: 3, Pages: 89-102
Endoscopy is the gold standard investigation in the diagnosis of gastrointestinal cancers and the management of early and pre-malignant lesions either by resection or ablation. Recently gold nanoparticles have shown promise in cancer diagnosis and therapeutics (theranostics). The combination of multifunctional gold nanoparticles with near infrared fluorescence endoscopy for accurate mapping of early or pre-malignant lesions can potentially enhance diagnostic efficiency while precisely directing endoscopic near infrared photothermal therapy for established cancers. The integration of endoscopy with near infrared fluorescence imaging and photothermal therapy was aided by the accumulation of our multifunctionalized PEG-GNR-Cy5.5-anti-EGFR-antibody gold nanorods within gastrointestinal tumor xenografts in BALB/c mice. Control mice (with tumors) received either gold nanorods or photothermal therapy, while study mice received both treatment modalities. Local (tumor-centric) and systemic effects were examined for 30 days. Clear endoscopic near infrared fluorescence signals were observed emanating specifically from tumor sites and these corresponded precisely to the tumor margins. Endoscopic fluorescence-guided near infrared photothermal therapy successfully induced tumor ablations in all 20 mice studied, with complete histological clearance and minimal collateral damage. Multi-source analysis from histology, electron microscopy, mass spectrometry, blood, clinical evaluation, psychosocial and weight monitoring demonstrated the inherent safety of this technology. The combination of this innovative nanotechnology with gold standard clinical practice will be of value in enhancing the early optical detection of gastrointestinal cancers and a useful adjunct for its therapy.
Loh AYY, Burgess CH, Tanase DA, et al., 2018, Electric Single-Molecule Hybridization Detector for Short DNA Fragments, ANALYTICAL CHEMISTRY, Vol: 90, Pages: 14063-14071, ISSN: 0003-2700
Piletsky SS, Cass AEG, Piletska E, et al., 2018, A Novel Assay Format as an Alternative to ELISA: MINA Test for Biotin, CHEMNANOMAT, Vol: 4, Pages: 1214-1222, ISSN: 2199-692X
Panagiotopoulos A, Gkouma A, Vassi A, et al., 2018, Hemin Modified SnO2 Films on ITO-PET with Enhanced Activity for Electrochemical Sensing, ELECTROANALYSIS, Vol: 30, Pages: 1956-1964, ISSN: 1040-0397
Rawson TM, Gowers S, Rogers M, et al., 2018, Towards a minimally invasive device for continuous monitoring of beta-lactam antibiotics, Publisher: ELSEVIER SCI LTD, Pages: 109-109, ISSN: 1201-9712
Maniyam MN, Ibrahim AL, Cass AEG, 2018, Decolourization and biodegradation of azo dye methyl red by Rhodococcus strain UCC 0016., Environ Technol, Pages: 1-15
In the present study, locally isolated Rhodococcus strains were attempted as biological tools for methyl red removal, a mutagenic azo dye posing threat to the environment if left untreated. Rhodococcus strain UCC 0016 demonstrated superior methyl red-decolourizing activity of 100% after 24 h at static condition in comparison to Rhodococcus strain UCC 0008 which recorded 65% decolourization after 72 h. Optimization of physicochemical parameters at 30°C, pH 7 and supplementing glucose as the carbon source resulted in improved methyl red-decolourizing activity at static condition and reduced the time taken to achieve complete decolourization by 80%. Higher concentration of methyl red (5 g/L) was able to be decolourized completely within 10 h by adopting the technology of immobilization. The encapsulated cells of Rhodococcus strain UCC 0016 demonstrated higher substrate affinity (Km = 0.6995 g/L) and an accelerated rate of disappearance of methyl red (Vmax = 0.3203 g/L/h) compared to the free cells. Furthermore, the gellan gum beads could be reused up to nine batches without substantial loss in the catalytic activity indicating the economic importance of this protocol. Analysis of methyl red degradation products revealed no germination inhibition on Triticum aestivum and Vigna radiata demonstrating complete toxicity removal of the parent dye after biological treatment. The occurrence of new and altered peaks (UV-Vis and FTIR) further supported the notion that the removal of methyl red by Rhodococcus strain UCC 0016 was indeed through biodegradation. Therefore, this strain has a huge potential as a candidate for efficient bioremediation of wastewater containing methyl red.
Sharma S, El-Laboudi A, Reddy M, et al., 2018, A pilot study in humans of microneedle sensor arrays for continuous glucose monitoring, ANALYTICAL METHODS, Vol: 10, Pages: 2088-2095, ISSN: 1759-9660
Rawson TM, O'Hare D, Herrero P, et al., 2018, Delivering precision antimicrobial therapy through closed-loop control systems, JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, Vol: 73, Pages: 835-843, ISSN: 0305-7453
Singh M, Nabavi E, Zhou Y, et al., Fluorescence image-guided photothermal therapy: Diagnosis and treatment of upper gastrointestinal cancer and beyond (prize winner), Global Surgery
Cass AEG, Hill HAO, 2018, Nuclear magnetic resonance spectroscopy of copper proteins, Copper Proteins and Copper Enzymes, Pages: 63-91, ISBN: 9781315891804
© 1984 by CRC Press, Inc. Of all the techniques currently used to study macromolecules in solution, only nuclear magnetic resonance (NMR) spectroscopy has the power to reveal details of molecular structure and motion at atomic resolution.1–5Though the complexity of the spectra of most molecules of interest makes it difficult to express this power and capitalize on the information provided, recent advances in spectrometer design and data manipulation have considerably extended its range of fruitful applications. Compare, e.g., an early (about 1975) 1H NMR spectrum of azurin with one obtained recently (Figure 1) in which the increased resolution and signal-to-noise ratio are only two of the improved features apparent. Many other advances will be revealed in examples considered in detail later in this chapter. First, we provide a brief description of the object of our spectroscopic attentions and the salient features of the technique for those unfortunate to have not yet made its acquaintance.
Sze JYY, Ivanov AP, Cass AEG, et al., 2017, Single molecule multiplexed nanopore protein screening in human serum using aptamer modified DNA carriers, NATURE COMMUNICATIONS, Vol: 8, ISSN: 2041-1723
Harris-Birtill D, Singh M, Zhou Y, et al., 2017, Gold nanorod reshaping in vitro and in vivo using a continuous wave laser, PLOS ONE, Vol: 12, ISSN: 1932-6203
Rawson TM, Sharma S, Georgiou P, et al., 2017, Towards a minimally invasive device for beta-lactam monitoring in humans, ELECTROCHEMISTRY COMMUNICATIONS, Vol: 82, Pages: 1-5, ISSN: 1388-2481
Le TT, Chang P, Benton DJ, et al., 2017, Dual Recognition Element Lateral Flow Assay Toward Multiplex Strain Specific Influenza Virus Detection, ANALYTICAL CHEMISTRY, Vol: 89, Pages: 6781-6786, ISSN: 0003-2700
Sharma S, Saeed A, Johnson C, et al., 2017, Rapid, low cost prototyping of transdermal devices for personal healthcare monitoring., Sens Biosensing Res, Vol: 13, Pages: 104-108, ISSN: 2214-1804
The next generation of devices for personal healthcare monitoring will comprise molecular sensors to monitor analytes of interest in the skin compartment. Transdermal devices based on microneedles offer an excellent opportunity to explore the dynamics of molecular markers in the interstitial fluid, however good acceptability of these next generation devices will require several technical problems associated with current commercially available wearable sensors to be overcome. These particularly include reliability, comfort and cost. An essential pre-requisite for transdermal molecular sensing devices is that they can be fabricated using scalable technologies which are cost effective. We present here a minimally invasive microneedle array as a continuous monitoring platform technology. Method for scalable fabrication of these structures is presented. The microneedle arrays were characterised mechanically and were shown to penetrate human skin under moderate thumb pressure. They were then functionalised and evaluated as glucose, lactate and theophylline biosensors. The results suggest that this technology can be employed in the measurement of metabolites, therapeutic drugs and biomarkers and could have an important role to play in the management of chronic diseases.
Cass AEG, Sharma S, 2017, Microneedle Enzyme Sensor Arrays for Continuous In Vivo Monitoring, ENZYMES AS SENSORS, Vol: 589, Pages: 413-427, ISSN: 0076-6879
Sharma S, Takagi E, Cass T, et al., 2017, Minimally invasive microneedle array electrodes employing direct electron transfer type glucose dehydrogenase for the development of continuous glucose monitoring sensors, BIOSENSORS 2016, Vol: 27, Pages: 208-209, ISSN: 2212-0173
Sharma S, Huang Z, Rogers M, et al., 2016, Evaluation of a minimally invasive glucose biosensor for continuous tissue monitoring, ANALYTICAL AND BIOANALYTICAL CHEMISTRY, Vol: 408, Pages: 8427-8435, ISSN: 1618-2642
Hughes JT, O'Dea K, Piera K, et al., 2016, Associations of serum adiponectin with markers of cardio-metabolic disease risk in Indigenous Australian adults with good health, diabetes and chronic kidney disease., Obes Res Clin Pract, Vol: 10, Pages: 659-672, ISSN: 1871-403X
The higher serum adiponectin concentrations observed in females are often attributed to differences in adiposity or sex hormones. There is little data describing adiponectin in Indigenous Australians, and no studies examining its association with cardio-metabolic disease risk markers and chronic kidney disease (CKD). AIM: To describe the relationship of serum adiponectin with cardio-metabolic disease risk markers and kidney function in a community-based sample of Indigenous Australian adults, with particular reference to sex-specific differences. METHODS: A cross-sectional analysis of a community-based volunteer sample of 548 Indigenous Australian adults (62% female), stratified into five cardio-metabolic risk groups ranging from good health (strata-1) to high cardio-metabolic risk and low measured glomerular filtration rate (mGFR, <60ml/min/1.73m2) (strata-5). We examined serum adiponectin concentrations with cardio-metabolic risk markers, albuminuria and mGFR. RESULTS: Indigenous Australian females had a lower than expected adiponectin concentration (3.5μg/ml), which was higher than males in strata 1-4 (as in other populations), but not in strata-5 (mGFR<60, p=0.19), and higher leptin: adiponectin ratio than other populations (7.8ng/μg - strata-1, healthy females; 12.2ng/μg - strata-3, females with diabetes and mGFR≥90). Female-gender, HDL-cholesterol (positive), mGFR and waist: hip ratio (WHR) (inverse) were independently associated with log-adiponectin when mGFR≥60; when mGFR<60, female-gender was associated with 0.27 units lower log-adiponectin. CONCLUSION: Female-gender was not associated with higher adiponectin concentrations in Indigenous Australians with mGFR<60ml/min/1.73m2. High WHR was frequent in both genders, and inversely associated with adiponectin. Longitudinal studies are needed to examine relationships of serum adiponectin, obesity and cardiovascular disease events in Indigenous Australians.
Badve SV, Palmer SC, Strippoli GFM, et al., 2016, The Validity of Left Ventricular Mass as a Surrogate End Point for All-Cause and Cardiovascular Mortality Outcomes in People With CKD: A Systematic Review and Meta-analysis., Am J Kidney Dis, Vol: 68, Pages: 554-563
BACKGROUND: Left ventricular mass (LVM) is a widely used surrogate end point in randomized trials involving people with chronic kidney disease (CKD) because treatment-induced LVM reductions are assumed to lower cardiovascular risk. The aim of this study was to assess the validity of LVM as a surrogate end point for all-cause and cardiovascular mortality in CKD. STUDY DESIGN: Systematic review and meta-analysis. SETTING & POPULATION: Participants with any stages of CKD. SELECTION CRITERIA FOR STUDIES: Randomized controlled trials with 3 or more months' follow-up that reported LVM data. INTERVENTION: Any pharmacologic or nonpharmacologic intervention. OUTCOMES: The surrogate outcome of interest was LVM change from baseline to last measurement, and clinical outcomes of interest were all-cause and cardiovascular mortality. Standardized mean differences (SMDs) of LVM change and relative risk for mortality were estimated using pairwise random-effects meta-analysis. Correlations between surrogate and clinical outcomes were summarized across all interventions combined using bivariate random-effects Bayesian models, and 95% credible intervals were computed. RESULTS: 73 trials (6,732 participants) covering 25 intervention classes were included in the meta-analysis. Overall, risk of bias was uncertain or high. Only 3 interventions reduced LVM: erythropoiesis-stimulating agents (9 trials; SMD, -0.13; 95% CI, -0.23 to -0.03), renin-angiotensin-aldosterone system inhibitors (13 trials; SMD, -0.28; 95% CI, -0.45 to -0.12), and isosorbide mononitrate (2 trials; SMD, -0.43; 95% CI, -0.72 to -0.14). All interventions had uncertain effects on all-cause and cardiovascular mortality. There were weak and imprecise associations between the effects of interventions on LVM change and all-cause (32 trials; 5,044 participants; correlation coefficient, 0.28; 95% credible interval, -0.13 to 0.59) and cardiovascular mortality (13 trials; 2,32
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