Imperial College London
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Professor Tony Cass

Faculty of Natural Sciences, Department of Chemistry

Senior Research Investigator
 
 
 
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Contact

 

+44 (0)20 7594 5195t.cass

 
 
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Location

 

301KMolecular Sciences Research HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Piletsky:2018:10.1002/cnma.201800393,
author = {Piletsky, SS and Cass, AEG and Piletska, EV and Czulak, J and Piletsky, SA},
doi = {10.1002/cnma.201800393},
journal = {ChemNanoMat},
pages = {1214--1222},
title = {A novel assay format as an alternative to ELISA: MINA test for biotin},
url = {http://dx.doi.org/10.1002/cnma.201800393},
volume = {4},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - A novel abiotic assay based on biotinspecific fluorescent molecularly imprinted polymer nanoparticles (nanoMIPs) which acted as both reporter probes and binding agents, was developed. This is a first report of an assay which, unlike ELISA, required no washing steps or addition of enzyme substrates, making it more userfriendly. The components of the molecularly imprinted polymer nanoparticles assay (MINA) were assembled in microtiter plates fitted with magnetic inserts. The fluorescent nanoMIPs were bound to biotinconjugated magnetic particles, which were attracted to the inserts. The addition of free biotin caused a displacement of the fluorescent nanoMIPs into solution, generating a signal proportional to the concentration of biotin. The nanoMIPs had a dissociation constant (Kd) of 14nM, allowing the assay to detect biotin at nanomolar concentrations. The preassembled assay only required the addition of the sample and measurement of the fluorescence, and it functioned well after six weeks of storage without refrigeration. The assay did not show the susceptibility to several compounds which are known to interfere with avidin and streptavidinbased assays, such as mercaptoethanol and sugars. The protocols optimized in this work could be used to develop the abiotic assays for any other compound of interest.
AU  - Piletsky,SS
AU  - Cass,AEG
AU  - Piletska,EV
AU  - Czulak,J
AU  - Piletsky,SA
DO  - 10.1002/cnma.201800393
EP  - 1222
PY  - 2018///
SN  - 2199-692X
SP  - 1214
TI  - A novel assay format as an alternative to ELISA: MINA test for biotin
T2  - ChemNanoMat
UR  - http://dx.doi.org/10.1002/cnma.201800393
UR  - http://hdl.handle.net/10044/1/63465
VL  - 4
ER  -
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