Imperial College London
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Professor Tony Cass

Faculty of Natural Sciences, Department of Chemistry

Senior Research Investigator
 
 
 
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Contact

 

+44 (0)20 7594 5195t.cass

 
 
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Location

 

301KMolecular Sciences Research HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Moreira:2014:10.1007/s00604-014-1409-0,
author = {Moreira, FTC and Sharma, S and Dutra, RAF and Noronha, JPC and Cass, AEG and Sales, MGF},
doi = {10.1007/s00604-014-1409-0},
journal = {Microchim Acta},
title = {Detection of cardiac biomarker proteins using a disposable basedon a molecularly imprinted polymer grafted onto graphite},
url = {http://dx.doi.org/10.1007/s00604-014-1409-0},
year = {2014}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - A low-cost disposable was developed for rapid detection of the protein biomarker myoglobin (Myo) as a model analyte. A screen printed electrode was modified with a molecularly imprinted material grafted on a graphite support and incorporated in a matrix composed of poly(vinyl chloride) and the plasticizer o-nitrophenyloctyl ether. The proteinimprinted material (PIM) was produced by growing a reticulated polymer around a protein template. This is followed by radical polymerization of 4-styrenesulfonic acid, 2- aminoethyl methacrylate hydrochloride, and ethylene glycol dimethacrylate. The polymeric layer was then covalently bound to the graphitic support, and Myo was added during the imprinting stage to act as a template. Non-imprinted control materials (CM) were also prepared by omitting the Myo template. Morphological and structural analysis of PIM and CM by FTIR, Raman, and SEM/EDC microscopies confirmed the modification of the graphite support. The analytical performance of the SPE was assessed by square wave voltammetry. The average limit of detection is 0.79 μg of Myo per mL, and the slope is −0.193±0.006 μA per decade. The SPECM cannot detect such low levels of Myo but gives a linearresponse at above 7.2 μg·mL−1, with a slope of −0.719±0.02 μA per decade. Interference studies with hemoglobin, bovine serum albumin, creatinine, and sodium chloride demonstrated good selectivity for Myo. The method was successfully applied to the determination of Myo urine and is conceived to be a promising tool for screening Myo in point-ofcare patients with ischemia.
AU  - Moreira,FTC
AU  - Sharma,S
AU  - Dutra,RAF
AU  - Noronha,JPC
AU  - Cass,AEG
AU  - Sales,MGF
DO  - 10.1007/s00604-014-1409-0
PY  - 2014///
TI  - Detection of cardiac biomarker proteins using a disposable basedon a molecularly imprinted polymer grafted onto graphite
T2  - Microchim Acta
UR  - http://dx.doi.org/10.1007/s00604-014-1409-0
UR  - http://download.springer.com/static/pdf/430/art%253A10.1007%252Fs00604-014-1409-0.pdf?auth66=1417776040_e3f773045cf47bf7e11280cc57fafeef&ext=.pdf
ER  -
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