Imperial College London
Portrait Picture

Tom Ellis

Faculty of Engineering, Department of Bioengineering

Professor of Synthetic Genome Engineering
 
 
 
//

Contact

 

+44 (0)20 7594 7615t.ellis Website CV

 
 
//

Location

 

704Bessemer BuildingSouth Kensington Campus

//

Summary

 

Publications

Citation

BibTex format

@article{McCarty:2019:10.1021/acssynbio.9b00041,
author = {McCarty, NS and Shaw, WM and Ellis, T and Ledesma-Amaro, R},
doi = {10.1021/acssynbio.9b00041},
journal = {ACS Synthetic Biology},
pages = {906--910},
title = {Rapid assembly of gRNA arrays via modular cloning in yeast},
url = {http://dx.doi.org/10.1021/acssynbio.9b00041},
volume = {8},
year = {2019}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - CRISPR is a versatile technology for genomic editing and regulation, but the expression of multiple gRNAs in S. cerevisiae has thus far been limited. We present here a simple extension to the Yeast MoClo Toolkit, which enables the rapid assembly of gRNA arrays using a minimal set of parts. Using a dual-PCR, Type IIs restriction enzyme Golden Gate assembly approach, at least 12 gRNAs can be assembled and expressed from a single transcriptional unit. We demonstrate that these gRNA arrays can stably regulate gene expression in a synergistic manner via dCas9-mediated repression. This approach expands the number of gRNAs that can be expressed in this model organism and may enable the versatile editing or transcriptional regulation of a greater number of genes in vivo.
AU  - McCarty,NS
AU  - Shaw,WM
AU  - Ellis,T
AU  - Ledesma-Amaro,R
DO  - 10.1021/acssynbio.9b00041
EP  - 910
PY  - 2019///
SN  - 2161-5063
SP  - 906
TI  - Rapid assembly of gRNA arrays via modular cloning in yeast
T2  - ACS Synthetic Biology
UR  - http://dx.doi.org/10.1021/acssynbio.9b00041
UR  - https://www.ncbi.nlm.nih.gov/pubmed/30939239
UR  - http://hdl.handle.net/10044/1/70031
VL  - 8
ER  -
Download