Imperial College London
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Tom Ellis

Faculty of Engineering, Department of Bioengineering

Professor of Synthetic Genome Engineering
 
 
 
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Contact

 

+44 (0)20 7594 7615t.ellis Website CV

 
 
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Location

 

704Bessemer BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Gilbert:2017:10.1021/acssynbio.6b00292,
author = {Gilbert, C and Howarth, M and Harwood, CR and Ellis, T},
doi = {10.1021/acssynbio.6b00292},
journal = {ACS Synthetic Biology},
pages = {957--967},
title = {Extracellular self-assembly of functional and tunable protein conjugates from Bacillus subtilis},
url = {http://dx.doi.org/10.1021/acssynbio.6b00292},
volume = {6},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The ability to stably and specifically conjugate recombinant proteins to one another is a powerful approach for engineering multifunctional enzymes, protein therapeutics, and novel biological materials. While many of these applications have been illustrated through in vitro and in vivo intracellular protein conjugation methods, extracellular self-assembly of protein conjugates offers unique advantages: simplifying purification, reducing toxicity and burden, and enabling tunability. Exploiting the recently described SpyTag-SpyCatcher system, we describe here how enzymes and structural proteins can be genetically encoded to covalently conjugate in culture media following programmable secretion from Bacillus subtilis. Using this approach, we demonstrate how self-conjugation of a secreted industrial enzyme, XynA, dramatically increases its resilience to boiling, and we show that cellular consortia can be engineered to self-assemble functional protein–protein conjugates with tunable composition. This novel genetically encoded modular system provides a flexible strategy for protein conjugation harnessing the substantial advantages of extracellular self-assembly.
AU  - Gilbert,C
AU  - Howarth,M
AU  - Harwood,CR
AU  - Ellis,T
DO  - 10.1021/acssynbio.6b00292
EP  - 967
PY  - 2017///
SN  - 2161-5063
SP  - 957
TI  - Extracellular self-assembly of functional and tunable protein conjugates from Bacillus subtilis
T2  - ACS Synthetic Biology
UR  - http://dx.doi.org/10.1021/acssynbio.6b00292
UR  - http://hdl.handle.net/10044/1/45032
VL  - 6
ER  -
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