Imperial College London
Portrait Picture

Tom Ellis

Faculty of Engineering, Department of Bioengineering

Professor of Synthetic Genome Engineering
 
 
 
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Contact

 

+44 (0)20 7594 7615t.ellis Website CV

 
 
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Location

 

704Bessemer BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Awan:2017:10.1038/ncomms15202,
author = {Awan, AR and Blount, BA and Bell, DJ and Shaw, WM and Ho, JCH and McKiernan, RM and Ellis, T},
doi = {10.1038/ncomms15202},
journal = {Nature Communications},
pages = {1--8},
title = {Biosynthesis of the antibiotic nonribosomal peptide penicillin in baker's yeast},
url = {http://dx.doi.org/10.1038/ncomms15202},
volume = {8},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Fungi are a valuable source of enzymatic diversity and therapeutic natural products including antibiotics. Here we engineer the baker’s yeast Saccharomyces cerevisiae to produce and secrete the antibiotic penicillin, a beta-lactam nonribosomal peptide, by taking genes from a filamentous fungus and directing their efficient expression and subcellular localization. Using synthetic biology tools combined with long-read DNA sequencing, we optimize productivity by 50-fold to produce bioactive yields that allow spent S. cerevisiae growth media to have antibacterial action against Streptococcus bacteria. This work demonstrates that S. cerevisiae can be engineered to perform the complex biosynthesis of multicellular fungi, opening up the possibility of using yeast to accelerate rational engineering of nonribosomal peptide antibiotics.
AU  - Awan,AR
AU  - Blount,BA
AU  - Bell,DJ
AU  - Shaw,WM
AU  - Ho,JCH
AU  - McKiernan,RM
AU  - Ellis,T
DO  - 10.1038/ncomms15202
EP  - 8
PY  - 2017///
SN  - 2041-1723
SP  - 1
TI  - Biosynthesis of the antibiotic nonribosomal peptide penicillin in baker's yeast
T2  - Nature Communications
UR  - http://dx.doi.org/10.1038/ncomms15202
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000400561400001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://www.nature.com/articles/ncomms15202
UR  - http://hdl.handle.net/10044/1/48888
VL  - 8
ER  -
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