Publications
413 results found
Liu Y, Feizi T, Campanero-Rhodes MA, et al., 2007, Neoglycolipid probes prepared via oxime ligation for microarray analysis of oligosaccharide-protein interactions, Chem.Biol., Vol: 14, Pages: 847-859
Neoglycolipid technology is the basis of a microarray platform for assigning oligosaccharide ligands for carbohydrate-binding proteins. The strategy for generating the neoglycolipid probes by reductive amination results in ring opening of the core monosaccharides. This often limits applicability to short-chain saccharides, although the majority of recognition motifs are satisfactorily presented with neoglycolipids of longer oligosaccharides. Here, we describe neoglycolipids prepared by oxime ligation. We provide evidence from NMR studies that a significant proportion of the oxime-linked core monosaccharide is in the ring-closed form, and this form selectively interacts with a carbohydrate-binding protein. By microarray analyses we demonstrate the effective presentation with oxime-linked neoglycolipids of (1) Lewis(x) trisaccharide to antibodies to Lewis(x), (2) sialyllactose analogs to the sialic acid-binding receptors, siglecs, and (3) N-glycans to a plant lectin that requires an intact N-acetylglucosamine core
Ewers H, Bacia K, Chai W, et al., 2007, The role of membrane lipid composition and tail structure in virus host cell association, entry, and infection., 61st Annual Meeting of the Society-of-General-Physiologists, Publisher: ROCKEFELLER UNIV PRESS, Pages: 3A-3A, ISSN: 0022-1295
Matthews S, 2007, Atomic resolution basis for host cell recognition by Toxoplasma gondii
Blumenschein TMA, Friedrich N, Childs RA, et al., 2007, Atomic resolution insight into host cell recognition by <i>Toxoplasma gondii</i>, EMBO JOURNAL, Vol: 26, Pages: 2808-2820, ISSN: 0261-4189
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- Citations: 86
Hamza D, Lucas R, Feizi T, et al., 2006, First synthesis of heparan sulfate tetrasaccha rides containing both <i>N</i>-acetylated and <i>N</i>-unsubstituted glucosamine -: Search for putative 10E4 epitopes, CHEMBIOCHEM, Vol: 7, Pages: 1856-1858, ISSN: 1439-4227
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- Citations: 16
Liu Y, Chai W, Crocker PR, et al., 2006, Neoglycolipids prepared via oxime-ligation for microarray analysis of carbohydrate-protein interactions, Meeting of the Society-for-Gylcobiology, Publisher: OXFORD UNIV PRESS INC, Pages: 1140-1140, ISSN: 0959-6658
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- Citations: 2
Campanero-Rhodes MA, Childs RA, Chai W, et al., 2006, A survey of Siglec binding preferences using carbohydrate microarrays, Meeting of the Society-for-Gylcobiology, Publisher: OXFORD UNIV PRESS INC, Pages: 1140-1140, ISSN: 0959-6658
Campanero-Rhodes MA, Childs RA, Kiso M, et al., 2006, Carbohydrate microarrays reveal sulphation as a modulator of siglec binding, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol: 344, Pages: 1141-1146, ISSN: 0006-291X
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- Citations: 80
Feizi T, 2006, Oligosaccharide microarrays to unravel carbohydrate recognition in the immune system, 31st Congress of the Federation-of-European-Biochemical-Societies (FEBS), Publisher: BLACKWELL PUBLISHING, Pages: 27-27, ISSN: 1742-464X
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- Citations: 1
Feizi T, 2006, Oligosaccharide microarrays to decipher the glyco code, 231st National Meeting of the American-Chemical-Society, Publisher: AMER CHEMICAL SOC, ISSN: 0065-7727
Palma AS, Feizi T, Zhang YB, et al., 2006, Ligands for the β-glucan receptor, Dectin-1, assigned using "designer" microarrays of oligosaccharide probes (neoglycolipids) generated from glucan polysaccharides, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 281, Pages: 5771-5779
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- Citations: 281
Liu Y, Chai W, Childs RA, et al., 2006, Preparation of neoglycolipids with ring-closed cores via chemoselective oxime-ligation for microarray analysis of carbohydrate-protein interactions, Methods Enzymol., Vol: 415, Pages: 326-340
Affinities of most oligosaccharide-protein interactions are so low that multivalent forms of ligand and protein are required for detecting interactions. The neoglycolipid (NGL) technology was designed to address the need for microscale presentation of oligosaccharides in a multivalent form for studying carbohydrate-protein interactions, and this is now the basis of a state-of-the-art carbohydrate microarray system. NGL technology involves conjugating oligosaccharides by reductive amination to the aminolipid 1,2-dihexadecyl-sn-glycero-3-phosphoethanolamine (DHPE). Other than ring-opening of the monosaccharide residues at reducing ends, oligosaccharides remain intact, and the NGLs derived from trisaccharides or larger oligosaccharides have performed well for the majority of carbohydrate-recognition systems that have the peripheral or backbone regions of oligosaccharides as recognition motifs. However, ring-opening of reducing end monosaccharides limits applicability to very short oligosaccharides (di- and trisaccharides) and, potentially, to N-glycans recognized by proteins such as Pisum sativum agglutinin (pea lectin) that require both intact core and backbone regions for strong binding. This chapter describes a method for preparing NGLs (designated AO-NGLs) from reducing oligosaccharides by chemoselective oxime-ligation to a new lipid reagent, N-aminooxyacetyl-DHPE. Microarray analyses of the AO-NGL derived from Lewis x (Le(x)) trisaccharide probed with anti-Le(x) antibodies indicate that a significant proportion of the core monosaccharide linked to lipid is in ring-closed form. Thus, AO-NGLs have broadened the applicability of NGLs as probes in studies of carbohydrate-protein interactions
Campanero-Rhodes MA, Crocker P, Childs RA, et al., 2005, Analyses of carbohydrate recognition by mammalian sialic acid-binding proteins of the immune system, siglecs, using microarrays of lipid-linked oligosaccharide probes, Meeting of the Society-for-Glycobiology, Publisher: OXFORD UNIV PRESS INC, Pages: 1188-1188, ISSN: 0959-6658
Feizi T, Mulloy B, 2005, Carbohydrates and glycoconjugates: Never a dull moment in glycoscience, Current Opinion in Structural Biology, Vol: 15, Pages: 479-480, ISSN: 0959-440X
Feizi T, Mulloy B, 2005, Carbohydrates and glycoconjugates: never a dull moment in glycoscience - Editorial overview, CURRENT OPINION IN STRUCTURAL BIOLOGY, Vol: 15, Pages: 479-480, ISSN: 0959-440X
Reddy ST, Chai W, Childs RA, et al., 2004, Identification of a Low Affinity Mannose 6-Phosphate Binding Site in Domain 5 of the Cation-Independent Mannose 6-Phosphate Receptor, J Biol Chem, Vol: 279, Pages: 38658-38667, ISSN: 0021-9258
Feizi T, 2004, Engineering sweet targets for magic bullets, NATURE BIOTECHNOLOGY, Vol: 22, Pages: 1244-1245, ISSN: 1087-0156
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- Citations: 1
Feizi T, 2004, Carbohydrate microarrays and the unravelling of ligands for effector proteins of the immune system, Meeting of the British-Society-for-Matrix-Biology, Publisher: BLACKWELL PUBLISHING LTD, Pages: A51-A52, ISSN: 0959-9673
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- Citations: 1
Chai WG, Leteux C, Westling C, et al., 2004, Relative susceptibilities of the glucosamine-glucuronic acid and <i>N</i>-acetylglucosamine-glucuronic acid linkages to heparin lyase III, BIOCHEMISTRY, Vol: 43, Pages: 8590-8599, ISSN: 0006-2960
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- Citations: 16
Feizi T, Chai WG, 2004, Oligosaccharide microarrays to decipher the glyco code, NATURE REVIEWS MOLECULAR CELL BIOLOGY, Vol: 5, Pages: 582-588, ISSN: 1471-0072
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- Citations: 200
Galustian C, Park CG, Chai WG, et al., 2004, High and low affinity carbohydrate ligands revealed for murine SIGN-R1 by carbohydrate array and cell binding approaches, and differing specificities for SIGN-R3 and langerin, INTERNATIONAL IMMUNOLOGY, Vol: 16, Pages: 853-866, ISSN: 0953-8178
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- Citations: 127
Reddy ST, Chai WG, Childs RA, et al., 2003, Identification of a low-affinity mannose 6-phosphate binding site in domain 5 of the cation-independent mannose 6-phosphate receptor, 8th Annual Conference of the Society-for-Glycobiology, Publisher: OXFORD UNIV PRESS INC, Pages: 869-869, ISSN: 0959-6658
Feizi T, Fazio F, Chai WC, et al., 2003, Carbohydrate microarrays - a new set of technologies at the frontiers of glycomics, CURRENT OPINION IN STRUCTURAL BIOLOGY, Vol: 13, Pages: 637-645, ISSN: 0959-440X
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- Citations: 260
Feizi T, Mulloy B, 2003, Carbohydrates and glycoconjugates glycomics: the new era of carbohydrate biology, CURRENT OPINION IN STRUCTURAL BIOLOGY, Vol: 13, Pages: 602-604, ISSN: 0959-440X
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- Citations: 35
Christine GA, Elviss N, Chart H, et al., 2003, Interactions of the gastrotropic bacterium <i>Helicobacter pylori</i> with the leukocyte-endothelium adhesion molecules, the selectins -: a preliminary report, FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY, Vol: 36, Pages: 127-134, ISSN: 0928-8244
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- Citations: 17
Feizi T, 2003, Oligosaccharide microarrays for glycomics, TRENDS IN BIOTECHNOLOGY, Vol: 21, Pages: 143-143, ISSN: 0167-7799
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- Citations: 5
Chai WG, Stoll MS, Galustian C, et al., 2003, Neoglycolipid technology: Deciphering information content of glycome, RECOGNITION OF CARBOHYDRATES IN BIOLOGICAL SYSTEMS PT A: GENERAL PROCEDURES, Vol: 362, Pages: 160-195, ISSN: 0076-6879
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- Citations: 56
Feizi T, Fukui S, Galustian C, et al., 2002, Progress toward deciphering the information content in the glycome, Pages: 61-62
The results of researches being carried out on 'glycome', a group of oligosaccharide structures found in the organism, were presented. The neoglycoplipid (NGL) technology, was developed for lipid-linked oligosaccharides probes from glycoproteins for studying their interactions with carbohydrate recognizing proteins. The uses of NGL technology as the basis of a carbohydrate microarray system for generating large repertoires of immobilized oligosaccharides probes required for the detection of carbohydrate protein interactions, were also discussed. It was shown that these repertoires of NGLs are robust probes when presented on nitrocellulose membranes.
Martín MJ, Feizi T, Leteux C, et al., 2002, An investigation of the interactions of E-selectin with fuco-oligosaccharides of the blood group family, GLYCOBIOLOGY, Vol: 12, Pages: 829-835, ISSN: 0959-6658
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- Citations: 11
Yamaguchi M, Ishida H, Galustian C, et al., 2002, Synthesis and selectin-binding activity of <i>N</i>-deacetylsialyl Lewis X ganglioside, CARBOHYDRATE RESEARCH, Vol: 337, Pages: 2111-2117, ISSN: 0008-6215
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- Citations: 8
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