Imperial College London
Alternate

ProfessorTenFeizi

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Professor and Director of the Glycosciences Laboratory
 
 
 
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Contact

 

+44 (0)20 7594 7207t.feizi

 
 
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Location

 

E518Burlington DanesHammersmith Campus

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Summary

 

Publications

Publication Type
Year
to

413 results found

Liu Y, Feizi T, Campanero-Rhodes MA, Childs RA, Zhang Y, Mulloy B, Evans PG, Osborn HM, Otto D, Crocker PR, Chai Wet al., 2007, Neoglycolipid probes prepared via oxime ligation for microarray analysis of oligosaccharide-protein interactions, Chem.Biol., Vol: 14, Pages: 847-859

Neoglycolipid technology is the basis of a microarray platform for assigning oligosaccharide ligands for carbohydrate-binding proteins. The strategy for generating the neoglycolipid probes by reductive amination results in ring opening of the core monosaccharides. This often limits applicability to short-chain saccharides, although the majority of recognition motifs are satisfactorily presented with neoglycolipids of longer oligosaccharides. Here, we describe neoglycolipids prepared by oxime ligation. We provide evidence from NMR studies that a significant proportion of the oxime-linked core monosaccharide is in the ring-closed form, and this form selectively interacts with a carbohydrate-binding protein. By microarray analyses we demonstrate the effective presentation with oxime-linked neoglycolipids of (1) Lewis(x) trisaccharide to antibodies to Lewis(x), (2) sialyllactose analogs to the sialic acid-binding receptors, siglecs, and (3) N-glycans to a plant lectin that requires an intact N-acetylglucosamine core

Journal article

Ewers H, Bacia K, Chai W, Schwarzmann G, Feizi T, Schwille P, Smith AE, Helenius Aet al., 2007, The role of membrane lipid composition and tail structure in virus host cell association, entry, and infection., 61st Annual Meeting of the Society-of-General-Physiologists, Publisher: ROCKEFELLER UNIV PRESS, Pages: 3A-3A, ISSN: 0022-1295

Conference paper

Matthews S, 2007, Atomic resolution basis for host cell recognition by Toxoplasma gondii

Conference paper

Blumenschein TMA, Friedrich N, Childs RA, Saouros S, Carpenter EP, Campanero-Rhodes MA, Simpson P, Chai W, Koutroukides T, Blackman MJ, Feizi T, Soldati-Favre D, Matthews Set al., 2007, Atomic resolution insight into host cell recognition by <i>Toxoplasma gondii</i>, EMBO JOURNAL, Vol: 26, Pages: 2808-2820, ISSN: 0261-4189

Journal article

Hamza D, Lucas R, Feizi T, Chai W, Bonnaffe D, Lubineau Aet al., 2006, First synthesis of heparan sulfate tetrasaccha rides containing both <i>N</i>-acetylated and <i>N</i>-unsubstituted glucosamine -: Search for putative 10E4 epitopes, CHEMBIOCHEM, Vol: 7, Pages: 1856-1858, ISSN: 1439-4227

Journal article

Liu Y, Chai W, Crocker PR, osborn HMI, Feizi Tet al., 2006, Neoglycolipids prepared via oxime-ligation for microarray analysis of carbohydrate-protein interactions, Meeting of the Society-for-Gylcobiology, Publisher: OXFORD UNIV PRESS INC, Pages: 1140-1140, ISSN: 0959-6658

Conference paper

Campanero-Rhodes MA, Childs RA, Chai W, Stoll MS, Crocker PR, Feizi Tet al., 2006, A survey of Siglec binding preferences using carbohydrate microarrays, Meeting of the Society-for-Gylcobiology, Publisher: OXFORD UNIV PRESS INC, Pages: 1140-1140, ISSN: 0959-6658

Conference paper

Campanero-Rhodes MA, Childs RA, Kiso M, Komba S, Le Narvor C, Warren J, Otto D, Crocker PR, Feizi Tet al., 2006, Carbohydrate microarrays reveal sulphation as a modulator of siglec binding, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol: 344, Pages: 1141-1146, ISSN: 0006-291X

Journal article

Feizi T, 2006, Oligosaccharide microarrays to unravel carbohydrate recognition in the immune system, 31st Congress of the Federation-of-European-Biochemical-Societies (FEBS), Publisher: BLACKWELL PUBLISHING, Pages: 27-27, ISSN: 1742-464X

Conference paper

Feizi T, 2006, Oligosaccharide microarrays to decipher the glyco code, 231st National Meeting of the American-Chemical-Society, Publisher: AMER CHEMICAL SOC, ISSN: 0065-7727

Conference paper

Palma AS, Feizi T, Zhang YB, Stoll MS, Lawson AM, Díaz-Rodríguez E, Campanero-Rhodes MA, Costa J, Gordon S, Brown GD, Chai WGet al., 2006, Ligands for the β-glucan receptor, Dectin-1, assigned using "designer" microarrays of oligosaccharide probes (neoglycolipids) generated from glucan polysaccharides, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 281, Pages: 5771-5779

Journal article

Liu Y, Chai W, Childs RA, Feizi Tet al., 2006, Preparation of neoglycolipids with ring-closed cores via chemoselective oxime-ligation for microarray analysis of carbohydrate-protein interactions, Methods Enzymol., Vol: 415, Pages: 326-340

Affinities of most oligosaccharide-protein interactions are so low that multivalent forms of ligand and protein are required for detecting interactions. The neoglycolipid (NGL) technology was designed to address the need for microscale presentation of oligosaccharides in a multivalent form for studying carbohydrate-protein interactions, and this is now the basis of a state-of-the-art carbohydrate microarray system. NGL technology involves conjugating oligosaccharides by reductive amination to the aminolipid 1,2-dihexadecyl-sn-glycero-3-phosphoethanolamine (DHPE). Other than ring-opening of the monosaccharide residues at reducing ends, oligosaccharides remain intact, and the NGLs derived from trisaccharides or larger oligosaccharides have performed well for the majority of carbohydrate-recognition systems that have the peripheral or backbone regions of oligosaccharides as recognition motifs. However, ring-opening of reducing end monosaccharides limits applicability to very short oligosaccharides (di- and trisaccharides) and, potentially, to N-glycans recognized by proteins such as Pisum sativum agglutinin (pea lectin) that require both intact core and backbone regions for strong binding. This chapter describes a method for preparing NGLs (designated AO-NGLs) from reducing oligosaccharides by chemoselective oxime-ligation to a new lipid reagent, N-aminooxyacetyl-DHPE. Microarray analyses of the AO-NGL derived from Lewis x (Le(x)) trisaccharide probed with anti-Le(x) antibodies indicate that a significant proportion of the core monosaccharide linked to lipid is in ring-closed form. Thus, AO-NGLs have broadened the applicability of NGLs as probes in studies of carbohydrate-protein interactions

Journal article

Campanero-Rhodes MA, Crocker P, Childs RA, Chai WG, Feizi Tet al., 2005, Analyses of carbohydrate recognition by mammalian sialic acid-binding proteins of the immune system, siglecs, using microarrays of lipid-linked oligosaccharide probes, Meeting of the Society-for-Glycobiology, Publisher: OXFORD UNIV PRESS INC, Pages: 1188-1188, ISSN: 0959-6658

Conference paper

Feizi T, Mulloy B, 2005, Carbohydrates and glycoconjugates: Never a dull moment in glycoscience, Current Opinion in Structural Biology, Vol: 15, Pages: 479-480, ISSN: 0959-440X

Journal article

Feizi T, Mulloy B, 2005, Carbohydrates and glycoconjugates: never a dull moment in glycoscience - Editorial overview, CURRENT OPINION IN STRUCTURAL BIOLOGY, Vol: 15, Pages: 479-480, ISSN: 0959-440X

Journal article

Reddy ST, Chai W, Childs RA, Page JD, Feizi T, Dahms NMet al., 2004, Identification of a Low Affinity Mannose 6-Phosphate Binding Site in Domain 5 of the Cation-Independent Mannose 6-Phosphate Receptor, J Biol Chem, Vol: 279, Pages: 38658-38667, ISSN: 0021-9258

Journal article

Feizi T, 2004, Engineering sweet targets for magic bullets, NATURE BIOTECHNOLOGY, Vol: 22, Pages: 1244-1245, ISSN: 1087-0156

Journal article

Feizi T, 2004, Carbohydrate microarrays and the unravelling of ligands for effector proteins of the immune system, Meeting of the British-Society-for-Matrix-Biology, Publisher: BLACKWELL PUBLISHING LTD, Pages: A51-A52, ISSN: 0959-9673

Conference paper

Chai WG, Leteux C, Westling C, Lindahl U, Feizi Tet al., 2004, Relative susceptibilities of the glucosamine-glucuronic acid and <i>N</i>-acetylglucosamine-glucuronic acid linkages to heparin lyase III, BIOCHEMISTRY, Vol: 43, Pages: 8590-8599, ISSN: 0006-2960

Journal article

Feizi T, Chai WG, 2004, Oligosaccharide microarrays to decipher the glyco code, NATURE REVIEWS MOLECULAR CELL BIOLOGY, Vol: 5, Pages: 582-588, ISSN: 1471-0072

Journal article

Galustian C, Park CG, Chai WG, Kiso M, Bruening SA, Kang YS, Steinman RM, Feizi Tet al., 2004, High and low affinity carbohydrate ligands revealed for murine SIGN-R1 by carbohydrate array and cell binding approaches, and differing specificities for SIGN-R3 and langerin, INTERNATIONAL IMMUNOLOGY, Vol: 16, Pages: 853-866, ISSN: 0953-8178

Journal article

Reddy ST, Chai WG, Childs RA, Feizi T, Dahms NMet al., 2003, Identification of a low-affinity mannose 6-phosphate binding site in domain 5 of the cation-independent mannose 6-phosphate receptor, 8th Annual Conference of the Society-for-Glycobiology, Publisher: OXFORD UNIV PRESS INC, Pages: 869-869, ISSN: 0959-6658

Conference paper

Feizi T, Fazio F, Chai WC, Wong CHet al., 2003, Carbohydrate microarrays - a new set of technologies at the frontiers of glycomics, CURRENT OPINION IN STRUCTURAL BIOLOGY, Vol: 13, Pages: 637-645, ISSN: 0959-440X

Journal article

Feizi T, Mulloy B, 2003, Carbohydrates and glycoconjugates glycomics: the new era of carbohydrate biology, CURRENT OPINION IN STRUCTURAL BIOLOGY, Vol: 13, Pages: 602-604, ISSN: 0959-440X

Journal article

Christine GA, Elviss N, Chart H, Owen R, Feizi Tet al., 2003, Interactions of the gastrotropic bacterium <i>Helicobacter pylori</i> with the leukocyte-endothelium adhesion molecules, the selectins -: a preliminary report, FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY, Vol: 36, Pages: 127-134, ISSN: 0928-8244

Journal article

Feizi T, 2003, Oligosaccharide microarrays for glycomics, TRENDS IN BIOTECHNOLOGY, Vol: 21, Pages: 143-143, ISSN: 0167-7799

Journal article

Chai WG, Stoll MS, Galustian C, Lawson AM, Feizi Tet al., 2003, Neoglycolipid technology: Deciphering information content of glycome, RECOGNITION OF CARBOHYDRATES IN BIOLOGICAL SYSTEMS PT A: GENERAL PROCEDURES, Vol: 362, Pages: 160-195, ISSN: 0076-6879

Journal article

Feizi T, Fukui S, Galustian C, Lawson AM, Chai Wet al., 2002, Progress toward deciphering the information content in the glycome, Pages: 61-62

The results of researches being carried out on 'glycome', a group of oligosaccharide structures found in the organism, were presented. The neoglycoplipid (NGL) technology, was developed for lipid-linked oligosaccharides probes from glycoproteins for studying their interactions with carbohydrate recognizing proteins. The uses of NGL technology as the basis of a carbohydrate microarray system for generating large repertoires of immobilized oligosaccharides probes required for the detection of carbohydrate protein interactions, were also discussed. It was shown that these repertoires of NGLs are robust probes when presented on nitrocellulose membranes.

Conference paper

Martín MJ, Feizi T, Leteux C, Pavlovic D, Piskarev VE, Chai WGet al., 2002, An investigation of the interactions of E-selectin with fuco-oligosaccharides of the blood group family, GLYCOBIOLOGY, Vol: 12, Pages: 829-835, ISSN: 0959-6658

Journal article

Yamaguchi M, Ishida H, Galustian C, Feizi T, Kiso Met al., 2002, Synthesis and selectin-binding activity of <i>N</i>-deacetylsialyl Lewis X ganglioside, CARBOHYDRATE RESEARCH, Vol: 337, Pages: 2111-2117, ISSN: 0008-6215

Journal article

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