Publications
562 results found
Douar AM, Adebakin S, Themis M, et al., 1997, Foetal gene delivery in mice by intra-amniotic administration of retroviral producer cells and adenovirus, GENE THERAPY, Vol: 4, Pages: 883-890, ISSN: 0969-7128
- Author Web Link
- Cite
- Citations: 65
Marshall BG, Wangoo A, Shaw RJ, et al., 1997, Growth factors and granulomatous inflammation - Reply, THORAX, Vol: 52, Pages: 588-588, ISSN: 0040-6376
- Author Web Link
- Cite
- Citations: 1
Chambers MA, Marshall BG, Wangoo A, et al., 1997, Differential responses to challenge with live and dead Mycobacterium bovis Bacillus Calmette-Guerin, JOURNAL OF IMMUNOLOGY, Vol: 158, Pages: 1742-1748, ISSN: 0022-1767
- Author Web Link
- Cite
- Citations: 24
Wangoo A, Laban C, Cook HT, et al., 1997, Interleukin-10- and corticosteroid-induced reduction in type I procollagen in a human ex vivo scar culture, INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY, Vol: 78, Pages: 33-41, ISSN: 0959-9673
- Author Web Link
- Cite
- Citations: 32
Ball S, Cook T, Hulme B, et al., 1997, The diagnosis and racial origin of 394 patients undergoing renal biopsy: an association between Indian race and interstitial nephritis., Nephrol Dial Transplant, Vol: 12, Pages: 71-77, ISSN: 0931-0509
BACKGROUND: There is a high incidence of renal disease in the ethnically Indian population in the United Kingdom, the pathological basis for which is only partly understood. This study attempted to define associations between renal biopsy diagnosis and race. The aim was thereby to identify types of renal disease which may contribute to the observed predisposition to renal failure in the Indian population served by our centre. METHOD: A single-centre-based retrospective analysis of the final diagnosis and corresponding ethnicity in 394 consecutive patients undergoing native renal biopsy for the investigation of abnormal renal function or urinary sediment. RESULTS: A highly significant association between a diagnosis of interstitial nephritis and Indian race was observed. There were 30 cases of interstitial nephritis, of whom 17 were Indian. In 15 of the Indian patients no aetiology could be established. The clinical features, outcomes, and the effect of steroid therapy in the Indian patients with idiopathic interstitial nephritis are described. CONCLUSION: Idiopathic interstitial nephritis is associated with Indian racial origin. This pathology may significantly contribute to the high incidence of end-stage renal failure in Indian patients resident in the United Kingdom.
Marshall BG, Wangoo A, Cook HT, et al., 1996, Increased inflammatory cytokines and new collagen formation in cutaneous tuberculosis and sarcoidosis, THORAX, Vol: 51, Pages: 1253-1261, ISSN: 0040-6376
- Author Web Link
- Cite
- Citations: 47
Cook HT, Cattell V, 1996, Role of nitric oxide in immune-mediated diseases, CLINICAL SCIENCE, Vol: 91, Pages: 375-384, ISSN: 0143-5221
- Author Web Link
- Cite
- Citations: 44
Wangoo A, Martin N, Cook HT, et al., 1996, Effect of injection of triamcinolone on type 1 procollagen and fibronectin in scar tissue, BRITISH JOURNAL OF SURGERY, Vol: 83, Pages: 1136-1137, ISSN: 0007-1323
- Author Web Link
- Cite
- Citations: 1
Cairns T, Lee J, Goldberg LC, et al., 1996, Thomsen-Friedenreich and PK antigens in pig-to-human xenotransplantation., Transplant Proc, Vol: 28, Pages: 795-796, ISSN: 0041-1345
Waddington S, Cook HT, Reaveley D, et al., 1996, L-arginine depletion inhibits glomerular nitric oxide synthesis and exacerbates rat nephrotoxic nephritis, KIDNEY INTERNATIONAL, Vol: 49, Pages: 1090-1096, ISSN: 0085-2538
- Author Web Link
- Cite
- Citations: 55
Bune AJ, Brand MP, Heales SJR, et al., 1996, Inhibition of tetrahydrobiopterin synthesis reduces in vivo nitric oxide production in experimental endotoxic shock, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol: 220, Pages: 13-19, ISSN: 0006-291X
- Author Web Link
- Cite
- Citations: 41
Bune AJ, Cook HT, 1996, Inhibition of tetrahydrobiopterin synthesis reduces nitric oxide production by isolated glomeruli in immune complex glomerulonephritis, EXPERIMENTAL NEPHROLOGY, Vol: 4, Pages: 43-47, ISSN: 1018-7782
- Author Web Link
- Cite
- Citations: 2
Cairns T, Lee J, Goldberg L, et al., 1995, Inhibition of the pig to human xenograft reaction, using soluble Gal alpha 1-3Gal and Gal alpha 1-3Gal beta 1-4GlcNAc., Transplantation, Vol: 60, Pages: 1202-1207, ISSN: 0041-1337
Natural anti-carbohydrate antibodies are central to hyperacute rejection in ABO-incompatible allotransplantation and in discordant xenotransplantation. ABO-incompatible rejection has been inhibited successfully using intravenous soluble carbohydrates as antibody inhibitors. The approach has been less successful previously in pig to primate xenotransplantation, where the necessary concentrations of a partial inhibitor (Gal alpha 1-6Glc) proved highly toxic. In this study, we have identified more effective inhibitors of the dominant human anti-pig antibodies that bind to the pentasaccharide Gal alpha 1-3Gal beta 1-4GlcNAc beta 1-3Gal beta 1-4Glc beta 1-. The inhibitors are the terminal disaccharide (Gal alpha 1-3Gal) and terminal trisaccharide (Gal alpha 1-3Gal beta 1-4GlcNAc) of the target pentasaccharide. Twelve sera (3 from each ABO blood group) were tested in 3 different assays: lymphocytotoxic, lymphocyte flow cytometry, and solid-phase antigen ELISA. Fifty percent to 75% inhibition of human IgG and IgM was achieved using the disaccharide and trisaccharide inhibitors in the range of 10-50 mM. Disaccharide (70 mM) was used to inhibit hyperacute thrombosis in pig kidneys perfused for 40 min with heparinized human AB whole blood. The disaccharide completely inhibited red cell occlusion of glomerular but not of intertubular capillaries, although there was residual platelet thrombus in glomeruli. Disaccharide and trisaccharide can, therefore, be used in concentrations shown for other carbohydrate inhibitors to be nontoxic, for inhibition of hyperacute pig-to-human xenograft rejection. The inhibition is incomplete, however, and other antigen specificities and other rejection mechanisms are likely to be involved.
Thomas DM, Wilkins MJ, Witana JS, et al., 1995, Giant cell reparative granuloma of the cricoid cartilage., J Laryngol Otol, Vol: 109, Pages: 1120-1123, ISSN: 0022-2151
Giant cell reparative granuloma (GCRG) is an uncommon benign lesion which has been reported at several sites in the head and neck. We present a case of a GCRG of the cricoid cartilage not previously described in the literature. It must be differentiated from the brown tumour of hyperparathyroidism and true giant cell tumours of bone. These were excluded on clinical, biochemical, radiological and histological grounds. The lesion responded well to surgical debulking and curettage and the patient remained disease-free 15 months after treatment.
Cattell V, Cook T, 1995, The nitric oxide pathway in glomerulonephritis., Curr Opin Nephrol Hypertens, Vol: 4, Pages: 359-364, ISSN: 1062-4821
Nitric oxide has fundamental roles in the modulation of many cell functions. In glomerulonephritis, generation of nitric oxide by the acutely inflamed glomerulus has recently been confirmed, with evidence that synthesis occurs through induction of the inducible form of nitric oxide synthase. Recent studies implicate infiltrating macrophages as a major source of this activity, although intrinsic glomerular cells may contribute as nitric oxide synthase has been found in cytokine-stimulated mesangial, endothelial and epithelial cells in culture. Cytokines, which are known to have a central role in glomerulonephritis, are the most probable stimulus of nitric oxide synthase induction in vivo, although this awaits confirmation. A role for nitric oxide in the pathophysiology of glomerulonephritis is not clearly defined at present but is strongly suggested by evidence for participation of nitric oxide in other immune and inflammatory diseases. The evidence evaluated in this review emphasizes that the role is certain to be complex. As yet it is not possible to predict whether the modulatory effects of nitric oxide on glomerular haemodynamics, vascular integrity, leucocyte infiltration and intrinsic glomerular cell responses are predominantly protective or cytotoxic. There are presently only two fully published reports of inhibition of nitric oxide in models of glomerulonephritis, one of which showed an exacerbation of acute heterologous nephrotoxic injury, and the other showed amelioration of chronic autoimmune glomerulonephritis. It is therefore premature to speculate on the effects of nitric oxide inhibition in glomerulonephritis. New insights await further understanding of the regulation of the inducible form of nitric oxide synthase in glomerulonephritis and the availability of specific inhibitors of this enzyme.
BUNE AJ, SHERGILL JK, CAMMACK R, et al., 1995, L-ARGININE DEPLETION BY ARGINASE REDUCES NITRIC-OXIDE PRODUCTION IN ENDOTOXIC-SHOCK - AN ELECTRON-PARAMAGNETIC-RESONANCE STUDY, FEBS LETTERS, Vol: 366, Pages: 127-130, ISSN: 0014-5793
- Author Web Link
- Cite
- Citations: 56
Wangoo A, Cook HT, Taylor GM, et al., 1995, Enhanced expression of type 1 procollagen and transforming growth factor-beta in tuberculin induced delayed type hypersensitivity., J Clin Pathol, Vol: 48, Pages: 339-345, ISSN: 0021-9746
AIMS: Tissue fibrosis is a common and serious consequence of chronic inflammation. The mechanism linking these two processes is poorly understood. The present study has utilised a human in vivo model of a delayed type hypersensitivity (DTH) reaction, the tuberculin Heaf reaction, induced by intradermal tuberculin in BCG immunised subjects, to dissect the relation between these two processes. METHODS: Punch skin biopsy specimens were obtained on day 5, day 13 and six to 16 weeks following the tuberculin Heaf test in 18 subjects with grade 3 or 4 responses. Skin biopsy specimens from six subjects served as controls. The specimens were examined using immunohistochemical staining for type 1 procollagen and transforming growth factor-beta (TGF-beta), as well as in situ hybridisation for type 1 procollagen messenger RNA (mRNA). RESULTS: Immunohistochemical analysis revealed increased deposition of TGF-beta in tissue matrix in the biopsy specimens obtained on day 5 following the tuberculin Heaf test. There was also extensive type 1 procollagen staining in the biopsy specimens obtained as early as day 5. Procollagen-1 staining was maximal on day 13, and was present in biopsy specimens from tuberculin Heaf test sites up to eight weeks after the tuberculin inoculation. The type 1 procollagen was localised within cells surrounding areas of inflammatory infiltrate and in perivascular tissues. The presence of new collagen formation was confirmed by in situ hybridisation using oligonucleotide probes for type 1 procollagen mRNA in cells in sections from biopsy specimens obtained on day 13. CONCLUSIONS: These data from a human in vivo model of a DTH response indicate that the immune response is intimately associated with an increase in the production of growth factors and the initiation of a fibrotic response.
Goldberg L, Lee J, Cairns T, et al., 1995, Inhibition of the human antipig xenograft reaction with soluble oligosaccharides., Transplant Proc, Vol: 27, Pages: 249-250, ISSN: 0041-1345
Riaz Y, Cook HT, Wangoo A, et al., 1994, Type 1 procollagen as a marker of severity of scarring after sternotomy: effects of topical corticosteroids., J Clin Pathol, Vol: 47, Pages: 892-899, ISSN: 0021-9746
AIMS: To determine whether the abundance of newly formed collagen in healing surgical wounds correlated with scar severity, and whether topical application of steroid cream reduced new collagen formation in patients who have undergone median sternotomy. METHODS: Thirty three patients six weeks after sternotomy, and 12 controls were studied. Scars were photographed, and biopsy specimens from scars at sites treated or untreated with topical corticosteroids (clobetasol proprionate 0.5%) were examined using immunohistochemical staining for type 1 procollagen (PCP 1) and transforming growth factor beta (TGF-beta), and in situ hybridisation for type 1 procollagen messenger RNA (mRNA). RESULTS: The degree of hypertrophy of the scar and the abundance of PCP 1 immunostaining were ranked independently, blind, and a correlation between these two variables was observed (r = 0.604, p < 0.001). The PCP 1 immunostaining was accompanied by a great abundance of PCP 1 mRNA and only a slight increase in TGF-beta immunostaining, when compared with normal skin or mature scars. Following the application of topical corticosteroids, for either 48 hours or twice daily for seven days, there was no reduction in PCP 1 immunostaining nor the abundance of PCP 1 mRNA. CONCLUSIONS: These data suggest that the extent of new collagen formation as assessed by PCP 1 immunohistochemistry may be a useful marker of the exuberance of the scarring process following sternotomy, and that topical corticosteroids are ineffective in reducing this component of the fibrotic response.
COOK HT, JANSEN A, LEWIS S, et al., 1994, ARGININE METABOLISM IN EXPERIMENTAL GLOMERULONEPHRITIS - INTERACTION BETWEEN NITRIC-OXIDE SYNTHASE AND ARGINASE, AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, Vol: 267, Pages: F646-F653, ISSN: 1931-857X
- Author Web Link
- Cite
- Citations: 60
COOK HT, EBRAHIM H, JANSEN AS, et al., 1994, EXPRESSION OF THE GENE FOR INDUCIBLE NITRIC-OXIDE SYNTHASE IN EXPERIMENTAL GLOMERULONEPHRITIS IN THE RAT, CLINICAL AND EXPERIMENTAL IMMUNOLOGY, Vol: 97, Pages: 315-320, ISSN: 0009-9104
- Author Web Link
- Cite
- Citations: 63
COOK HT, BUNE AJ, JANSEN AS, et al., 1994, CELLULAR-LOCALIZATION OF INDUCIBLE NITRIC-OXIDE SYNTHASE IN EXPERIMENTAL ENDOTOXIC-SHOCK IN THE RAT, CLINICAL SCIENCE, Vol: 87, Pages: 179-186, ISSN: 0143-5221
- Author Web Link
- Cite
- Citations: 101
COOK HT, AVEY C, TAYLOR GM, 1994, INTERLEUKIN-1-BETA GENE-EXPRESSION IN EXPERIMENTAL GLOMERULONEPHRITIS IN THE RAT - AN IN-SITU HYBRIDIZATION STUDY, INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY, Vol: 75, Pages: 157-163, ISSN: 0959-9673
- Author Web Link
- Cite
- Citations: 6
JANSEN A, COOK T, TAYLOR GM, et al., 1994, INDUCTION OF NITRIC-OXIDE SYNTHASE IN RAT IMMUNE-COMPLEX GLOMERULONEPHRITIS, KIDNEY INTERNATIONAL, Vol: 45, Pages: 1215-1219, ISSN: 0085-2538
- Author Web Link
- Cite
- Citations: 107
Toynton SC, Wilkins MJ, Cook HT, et al., 1994, True malignant mixed tumour of a minor salivary gland., J Laryngol Otol, Vol: 108, Pages: 76-79, ISSN: 0022-2151
True malignant mixed tumours (carcinosarcomas) of salivary gland origin are rare and not widely recognized lesions. These tumours account for approximately 0.2 per cent of all malignancies of the major salivary glands (Stephen et al., 1986). They usually occur in pre-existing pleomorphic adenomas of major salivary glands and have an aggressive and often rapidly fatal progression. We report a case of carcinosarcoma in a minor salivary gland of the upper lip. Its management and the use of immunocytochemical stains to demonstrate the separate epithelial and connective issue components are discussed.
Lianos EA, Orphanos V, Cattell V, et al., 1994, Glomerular expression and cell origin of transforming growth factor-beta 1 in anti-glomerular basement membrane disease., Am J Med Sci, Vol: 307, Pages: 1-5, ISSN: 0002-9629
The glomerular expression (mRNA levels) of transforming growth factor-beta 1 (TGF-beta 1) was assessed in two forms of rat anti-glomerular basement membrane (GBM) disease, a macrophage-independent and a macrophage-dependent variant. After a single intravenous injection of rabbit anti-rat GBM immune serum, significant proteinuria and histopathologic changes developed in both variants. Increased TGF-beta 1 mRNA levels were found in isolated glomeruli of the macrophage-dependent variant only in which glomerular infiltration by macrophages also occurred. Macrophages isolated from glomeruli of animals with this variant demonstrated TGF-beta 1 mRNA levels comparable to those found in glomeruli isolated at the same time point after injection of the anti-GBM serum. The observations indicate that in anti-GBM disease, enhanced glomerular TGF-beta 1 expression occurs in the macrophage-dependent variant and suggest that infiltrating macrophages account for this event.
Goldberg LC, Cook T, Taube D, 1994, Pretreatment of renal transplants with anti-CD45 antibodies: optimization of perfusion technique., Transpl Immunol, Vol: 2, Pages: 27-34, ISSN: 0966-3274
Pig kidneys were perfused ex vivo with a monoclonal anti-pig CD45 antibody to determine optimum conditions for human kidney anti-CD45 perfusion, as such pretreatment may prolong renal allograft survival. Efficacy of perfused antibody uptake was assessed by immunohistochemical double-labelling of multiple renal biopsies. Antibody uptake by CD45+ cells was rapid, and maximal within one hour of perfusion. An antibody concentration of 40 micrograms/ml more effectively saturated available CD45 binding sites than lower concentrations. Whilst cortical perfusion was homogeneous (median uptake > 99%, minimum 90.0%) after 25, 50 or 100 ml perfusion with 40 micrograms/ml monoclonal antibody (mAb), the medulla was less evenly perfused with 25 ml (median uptake 82.6%) than with 50 or 100 ml (median uptake 98.5% and 98.7%, respectively). Prolongation of renal cold ischaemia of up to 46 hours prior to mAb perfusion did not adversely affect antibody binding. Clamping of the renal vein during and after mAb perfusion was associated with better cortical and medullary mAb uptake than if the vein was left unclamped. These data show that the vast majority of the kidney's CD45+ antigen-presenting cells can be bound by anti-CD45 mAb perfused ex vivo, if an adequate volume and concentration of mAb perfusate is employed and the renal vein is clamped during and after the process.
Thom M, Palmer A, Cattell V, et al., 1994, Proliferating cell nuclear antigen (PCNA) as a diagnostic marker of acute cellular rejection in routinely processed biopsies of renal allografts., Nephrol Dial Transplant, Vol: 9, Pages: 153-155, ISSN: 0931-0509
Seventy-eight renal allograft biopsies taken for the management of graft dysfunction were analysed retrospectively by labelling with antibodies to proliferating cell nuclear antigen (PCNA) to assess whether this marker could distinguish episodes of rejection. Routinely processed, paraffin-embedded biopsies with focal or diffuse cellular infiltrates were selected and the percentage of infiltrating cells in the cortical interstitium which stained with PCNA antibody were counted (PCNA index). The area of cellular infiltration was also estimated by a morphometric point-counting technique. The biopsies were divided into two groups based on standard clinical criteria; those with acute rejection (n = 31) and those with other causes of graft dysfunction (n = 47). The PCNA index was significantly higher in episodes of acute rejection (7.9%) compared to non-rejection (2.1%). This was independent of time after transplantation. The PCNA index was also greater in rejecting kidneys containing only focal cellular infiltrates (percentage area of cellular infiltration < 13.1%). Thus PCNA staining may be of use in the differential diagnosis of rejection in routinely processed biopsies of renal allografts where there are only focal inflammatory infiltrates, otherwise not diagnostic of cellular rejection.
CATTELL V, COOK HT, 1993, NITRIC-OXIDE - ROLE IN THE PHYSIOLOGY AND PATHOLOGY OF THE GLOMERULUS, EXPERIMENTAL NEPHROLOGY, Vol: 1, Pages: 265-280, ISSN: 1018-7782
- Author Web Link
- Cite
- Citations: 64
Goldberg LC, Cook HT, Welsh KI, et al., 1993, Optimizing kidney perfusion with anti-CD45 monoclonal antibody., Transplant Proc, Vol: 25, Pages: 1056-1057, ISSN: 0041-1345
This data is extracted from the Web of Science and reproduced under a licence from Thomson Reuters. You may not copy or re-distribute this data in whole or in part without the written consent of the Science business of Thomson Reuters.