Imperial College London
Alternate

PROFESSOR H. TERENCE COOK

Faculty of Medicine, Department of Immunology and Inflammation

Emeritus Professor
 
 
 
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Contact

 

+44 (0)20 3313 2009t.h.cook

 
 
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Assistant

 

Miss Claudia Rocchi +44 (0)20 3313 2315

 
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Location

 

9N9Commonwealth BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Hamaoui:2020:10.1016/j.ijso.2019.10.002,
author = {Hamaoui, K and Gowers, S and Sandhu, B and Cook, T and Boutelle, M and Casanova-Rituerto, D and Papalois, V},
doi = {10.1016/j.ijso.2019.10.002},
journal = {International Journal of Surgery Open},
pages = {39--47},
title = {Cold ischaemia time: is too long really too bad? studies using a porcine kidney ex-vivo reperfusion model},
url = {http://dx.doi.org/10.1016/j.ijso.2019.10.002},
volume = {23},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - IntroductionPost-ischaemic hypothermic machine perfusion (HMP) may be beneficial in recovery of marginal kidney grafts. The full capacity of conventional HMP (with passive oxygenation) to recondition an organ has not been realised. We investigated whether HMP can ameliorate ischemic damage caused by extremely prolonged static cold storage (SCS).MethodsPorcine kidneys underwent 4-h (SCS4,n = 4) or 52-h (SCS52,n = 4) SCS, followed by 10 h of HMP and were then subjected to 2 h of isolated normothermic reperfusion (NRP).ResultsThere was a post-SCS graft weight loss in SCS52 vs SCS4 kidneys. SCS52 kidneys showed viable perfusion dynamics during HMP, with significantly shorter times to reach viable parameters vs SCS4 kidneys (p < 0.027). During NRP SCS52 kidneys demonstrated similar trends in perfusion dynamics, renal function, oxygen consumptions, lactate production, and tubular injury to SCS4 kidneys.ConclusionGraft weight loss after SCS, reducing resistance to perfusion, may facilitate better HMP dynamics and graft reconditioning. Clinicians utilising HMP should be aware of this phenomenon when using HMP in kidneys exposed to extreme periods of SCS. HMP after an extended period of SCS can resuscitate kidneys to a level equitable of viability as those after a short period of SCS. Utilising passive oxygenation however may be limiting such recovery and interventions utilising active oxygenation may provide benefit in such organs.
AU  - Hamaoui,K
AU  - Gowers,S
AU  - Sandhu,B
AU  - Cook,T
AU  - Boutelle,M
AU  - Casanova-Rituerto,D
AU  - Papalois,V
DO  - 10.1016/j.ijso.2019.10.002
EP  - 47
PY  - 2020///
SN  - 2405-8572
SP  - 39
TI  - Cold ischaemia time: is too long really too bad? studies using a porcine kidney ex-vivo reperfusion model
T2  - International Journal of Surgery Open
UR  - http://dx.doi.org/10.1016/j.ijso.2019.10.002
UR  - https://www.sciencedirect.com/science/article/pii/S2405857219300592?via%3Dihub
UR  - http://hdl.handle.net/10044/1/75803
VL  - 23
ER  -
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