Imperial College London

ProfessorAnthonyMagee

Faculty of MedicineNational Heart & Lung Institute

Chair in Membrane Biology
 
 
 
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Contact

 

t.magee Website

 
 
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Location

 

Office no. 106Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Publication Type
Year
to

186 results found

Lanyon-Hogg T, Patel NV, Ritzefeld M, Boxall KJ, Burke R, Blagg J, Magee AI, Tate EWet al., 2017, Microfluidic Mobility Shift Assay for Real-Time Analysis of Peptide N-Palmitoylation, SLAS DISCOVERY, Vol: 22, Pages: 418-424, ISSN: 2472-5552

JOURNAL ARTICLE

Lanyon-Hogg T, Masumoto N, Bodakh G, Konitsiotis AD, Thinon E, Rodgers UR, Owens RJ, Magee AI, Tate EWet al., 2016, Synthesis and characterisation of 5-acyl-6,7-dihydrothieno[3,2-c]pyridine inhibitors of Hedgehog acyltransferase., Data Brief, Vol: 7, Pages: 257-281, ISSN: 2352-3409

In this data article we describe synthetic and characterisation data for four members of the 5-acyl-6,7-dihydrothieno[3,2-c]pyridine (termed "RU-SKI") class of inhibitors of Hedgehog acyltransferase, including associated NMR spectra for final compounds. RU-SKI compounds were selected for synthesis based on their published high potencies against the enzyme target. RU-SKI 41 (9a), RU-SKI 43 (9b), RU-SKI 101 (9c), and RU-SKI 201 (9d) were profiled for activity in the related article "Click chemistry armed enzyme linked immunosorbent assay to measure palmitoylation by Hedgehog acyltransferase" (Lanyon-Hogg et al., 2015) [1]. (1)H NMR spectral data indicate different amide conformational ratios between the RU-SKI inhibitors, as has been observed in other 5-acyl-6,7-dihydrothieno[3,2-c]pyridines. The synthetic and characterisation data supplied in the current article provide validated access to the class of RU-SKI inhibitors.

JOURNAL ARTICLE

Rodgers UR, Lanyon-Hogg T, Masumoto N, Ritzefeld M, Burke R, Blagg J, Magee AI, Tate EWet al., 2016, Characterization of Hedgehog Acyltransferase Inhibitors Identifies a Small Molecule Probe for Hedgehog Signaling by Cancer Cells, ACS CHEMICAL BIOLOGY, Vol: 11, Pages: 3256-3262, ISSN: 1554-8929

JOURNAL ARTICLE

Broncel M, Serwa RA, Ciepla P, Krause E, Dallman MJ, Magee AI, Tate EWet al., 2015, Multifunctional Reagents for Quantitative Proteome-Wide Analysis of Protein Modification in Human Cells and Dynamic Profiling of Protein Lipidation During Vertebrate Development, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, Vol: 54, Pages: 5948-5951, ISSN: 1433-7851

JOURNAL ARTICLE

Broncel M, Serwa RA, Ciepla P, Krause E, Dallman MJ, Magee AI, Tate EWet al., 2015, Myristoylation profiling in human cells and zebrafish., Data Brief, Vol: 4, Pages: 379-383, ISSN: 2352-3409

Human cells (HEK 293, HeLa, MCF-7) and zebrafish embryos were metabolically tagged with an alkynyl myristic acid probe, lysed with an SDS buffer and tagged proteomes ligated to multifunctional capture reagents via copper-catalyzed alkyne azide cycloaddition (CuAAC). This allowed for affinity enrichment and high-confidence identification, by delivering direct MS/MS evidence for the modification site, of 87 and 61 co-translationally myristoylated proteins in human cells and zebrafish, respectively. The data have been deposited to ProteomeXchange Consortium (Vizcaíno et al., 2014 Nat. Biotechnol., 32, 223-6) (PXD001863 and PXD001876) and are described in detail in Multifunctional reagents for quantitative proteome-wide analysis of protein modification in human cells and dynamic protein lipidation during vertebrate development׳ by Broncel et al., Angew. Chem. Int. Ed.

JOURNAL ARTICLE

Ciepla P, Magee AI, Tate EW, 2015, Cholesterylation: a tail of hedgehog, BIOCHEMICAL SOCIETY TRANSACTIONS, Vol: 43, Pages: 262-267, ISSN: 0300-5127

JOURNAL ARTICLE

Koleva MV, Rothery S, Spitaler M, Neil MAA, Magee AIet al., 2015, Sonic hedgehog multimerization: A self-organizing event driven by post-translational modifications?, MOLECULAR MEMBRANE BIOLOGY, Vol: 32, Pages: 65-74, ISSN: 0968-7688

JOURNAL ARTICLE

Konitsiotis AD, Jovanovic B, Ciepla P, Spitaler M, Lanyon-Hogg T, Tate EW, Magee AIet al., 2015, Topological Analysis of Hedgehog Acyltransferase, a Multipalmitoylated Transmembrane Protein, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 290, Pages: 3293-3307, ISSN: 0021-9258

JOURNAL ARTICLE

Lanyon-Hogg T, Masumoto N, Bodakh G, Konitsiotis AD, Thinon E, Rodgers UR, Owens RJ, Magee AI, Tate EWet al., 2015, Click chemistry armed enzyme-linked immunosorbent assay to measure palmitoylation by hedgehog acyltransferase, ANALYTICAL BIOCHEMISTRY, Vol: 490, Pages: 66-72, ISSN: 0003-2697

JOURNAL ARTICLE

Lanyon-Hogg T, Ritzefeld M, Masumoto N, Magee AI, Rzepa HS, Tate EWet al., 2015, Modulation of Amide Bond Rotamers in 5-Acyl-6,7-dihydrothieno[3,2-c]pyridines, JOURNAL OF ORGANIC CHEMISTRY, Vol: 80, Pages: 4370-4377, ISSN: 0022-3263

JOURNAL ARTICLE

Magee T, Henderson P, Baker A, Postis V, Muench Set al., 2015, A Tribute to Stephen Allan Baldwin, MOLECULAR MEMBRANE BIOLOGY, Vol: 32, Pages: 33-34, ISSN: 0968-7688

JOURNAL ARTICLE

Masumoto N, Lanyon-Hogg T, Rodgers UR, Konitsiotis AD, Magee AI, Tate EWet al., 2015, Membrane bound O-acyltransferases and their inhibitors, BIOCHEMICAL SOCIETY TRANSACTIONS, Vol: 43, Pages: 246-252, ISSN: 0300-5127

JOURNAL ARTICLE

Ciepla P, Konitsiotis AD, Serwa RA, Masumoto N, Leong WP, Dallman MJ, Magee AI, Tate EWet al., 2014, New chemical probes targeting cholesterylation of Sonic Hedgehog in human cells and zebrafish, Chemical Science, Vol: 5, Pages: 4249-4259, ISSN: 2041-6520

Sonic Hedgehog protein (Shh) is a morphogen molecule important in embryonic development and in theprogression of many cancer types in which it is aberrantly overexpressed. Fully mature Shh requiresattachment of cholesterol and palmitic acid to its C- and N-termini, respectively. The study of lipidatedShh has been challenging due to the limited array of tools available, and the roles of theseposttranslational modifications are poorly understood. Herein, we describe the development andvalidation of optimised alkynyl sterol probes that efficiently tag Shh cholesterylation and enable itsvisualisation and analysis through bioorthogonal ligation to reporters. An optimised probe was shown tobe an excellent cholesterol biomimetic in the context of Shh, enabling appropriate release of tagged Shhfrom signalling cells, formation of multimeric transport complexes and signalling. We have used thisprobe to determine the size of transport complexes of lipidated Shh in culture medium and expressionlevels of endogenous lipidated Shh in pancreatic ductal adenocarcinoma cell lines through quantitativechemical proteomics, as well as direct visualisation of the probe by fluorescence microscopy anddetection of cholesterylated Hedgehog protein in developing zebrafish embryos. These sterol probesprovide a set of novel and well-validated tools that can be used to investigate the role of lipidation onactivity of Shh, and potentially other members of the Hedgehog protein family

JOURNAL ARTICLE

Konitsiotis AD, Chang S-C, Jovanovic B, Ciepla P, Masumoto N, Palmer CP, Tate EW, Couchman JR, Magee AIet al., 2014, Attenuation of Hedgehog Acyltransferase-Catalyzed Sonic Hedgehog Palmitoylation Causes Reduced Signaling, Proliferation and Invasiveness of Human Carcinoma Cells, PLOS ONE, Vol: 9, ISSN: 1932-6203

JOURNAL ARTICLE

McDonald G, Deepak S, Miguel L, Hall CJ, Isenberg DA, Magee AI, Butters T, Jury ECet al., 2014, Normalizing glycosphingolipids restores function in CD4(+) T cells from lupus patients, JOURNAL OF CLINICAL INVESTIGATION, Vol: 124, Pages: 712-724, ISSN: 0021-9738

JOURNAL ARTICLE

Konitsiotis A, Chang S, Masumoto N, Tate EW, Magee AIet al., 2012, Hhat a Potential New Target in Treatment of Pancreatic Cancer, 22nd Biennial Congress of the European-Association-for-Cancer-Research, Publisher: ELSEVIER SCI LTD, Pages: S149-S149, ISSN: 0959-8049

CONFERENCE PAPER

McDonald G, Miguel L, Hall C, Isenberg DA, Magee AI, Butters T, Jury ECet al., 2012, Targeting Glycosphingolipid Biosynthesis Normalises T Lymphocyte Function in Patients with Systemic Lupus Eyrthematosus, Annual Scientific Meeting of the American-College-of-Rheumatology (ACR) and Association-of-Rheumatology-Health-Professionals (ARHP), Publisher: WILEY-BLACKWELL, Pages: S370-S370, ISSN: 0004-3591

CONFERENCE PAPER

Vincent J-P, Magee T, 2012, Tiki Casts a Spell on Wnt, CELL, Vol: 149, Pages: 1426-1427, ISSN: 0092-8674

JOURNAL ARTICLE

Chang S-C, Mulloy B, Magee AI, Couchman JRet al., 2011, Two Distinct Sites in Sonic Hedgehog Combine for Heparan Sulfate Interactions and Cell Signaling Functions, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 286, Pages: 44391-44402, ISSN: 0021-9258

JOURNAL ARTICLE

Heal WP, Jovanovic B, Bessin S, Wright MH, Magee AI, Tate EWet al., 2011, Bioorthogonal chemical tagging of protein cholesterylation in living cells, CHEMICAL COMMUNICATIONS, Vol: 47, Pages: 4081-4083, ISSN: 1359-7345

JOURNAL ARTICLE

Miguel L, Owen DM, Lim C, Liebig C, Evans J, Magee AI, Jury ECet al., 2011, Primary Human CD4(+) T Cells Have Diverse Levels of Membrane Lipid Order That Correlate with Their Function, JOURNAL OF IMMUNOLOGY, Vol: 186, Pages: 3505-3516, ISSN: 0022-1767

JOURNAL ARTICLE

Plaza-Menacho I, Morandi A, Mologni L, Boender P, Gambacorti-Passerini C, Magee AI, Hofstra RMW, Knowles P, McDonald NQ, Isacke CMet al., 2011, Focal Adhesion Kinase (FAK) Binds RET Kinase via Its FERM Domain, Priming a Direct and Reciprocal RET-FAK Transactivation Mechanism, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 286, Pages: 17292-17302, ISSN: 0021-9258

JOURNAL ARTICLE

Salmond RJ, Filby A, Pirinen N, Magee AI, Zamoyska Ret al., 2011, Mislocalization of Lck impairs thymocyte differentiation and can promote development of thymomas, BLOOD, Vol: 117, Pages: 108-117, ISSN: 0006-4971

JOURNAL ARTICLE

Zeidman R, Buckland G, Cebecauer M, Eissmann P, Davis DM, Magee AIet al., 2011, DHHC2 is a protein S-acyltransferase for Lck, MOLECULAR MEMBRANE BIOLOGY, Vol: 28, Pages: 473-486, ISSN: 0968-7688

JOURNAL ARTICLE

Cebecauer M, Spitaler M, Serge A, Magee AIet al., 2010, Signalling complexes and clusters: functional advantages and methodological hurdles, JOURNAL OF CELL SCIENCE, Vol: 123, Pages: 309-320, ISSN: 0021-9533

JOURNAL ARTICLE

Miguel L, Owen DM, Lim C, Magee AI, Jury ECet al., 2010, Primary human CD4(+) T cells have diverse levels of membrane lipid order that dictate their function, Annual Congress of the British-Society-for-Immunology, Publisher: WILEY-BLACKWELL, Pages: 77-77, ISSN: 0019-2805

CONFERENCE PAPER

Owen DM, Gaus K, Magee AI, Cebecauer Met al., 2010, Dynamic organization of lymphocyte plasma membrane: lessons from advanced imaging methods, IMMUNOLOGY, Vol: 131, Pages: 1-8, ISSN: 0019-2805

JOURNAL ARTICLE

Owen DM, Oddos S, Kumar S, Davis DM, Neil MAA, French PMW, Dustin ML, Magee AI, Cebecauer Met al., 2010, High plasma membrane lipid order imaged at the immunological synapse periphery in live T cells, MOLECULAR MEMBRANE BIOLOGY, Vol: 27, Pages: 178-189, ISSN: 0968-7688

JOURNAL ARTICLE

Breckenridge RA, Zuberi Z, Gomes J, Orford R, Dupays L, Felkin LE, Clark JE, Magee AI, Ehler E, Birks EJ, Barton PJR, Tinker A, Mohun TJet al., 2009, Overexpression of the transcription factor Hand1 causes predisposition towards arrhythmia in mice, JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, Vol: 47, Pages: 133-141, ISSN: 0022-2828

JOURNAL ARTICLE

Cebecauer M, Owen DM, Markiewicz A, Magee AIet al., 2009, Lipid order and molecular assemblies in the plasma membrane of eukaryotic cells, BIOCHEMICAL SOCIETY TRANSACTIONS, Vol: 37, Pages: 1056-1060, ISSN: 0300-5127

JOURNAL ARTICLE

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