Imperial College London

Tony D. Southall

Faculty of Natural SciencesDepartment of Life Sciences

Senior Lecturer
 
 
 
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Contact

 

+44 (0)20 7594 5338t.southall

 
 
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Location

 

220 off lab 216Sir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Southall:2018:10.7554/eLife.32341,
author = {Southall, TD and Aughey, GN and Estacio, Gomez A and Yin, H and Thomson, J},
doi = {10.7554/eLife.32341},
journal = {eLife},
title = {CATaDa reveals global remodelling of chromatin accessibility during stem cell differentiation in vivo},
url = {http://dx.doi.org/10.7554/eLife.32341},
volume = {7},
year = {2018}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - During development eukaryotic gene expression is coordinated by dynamic changes in chromatin structure. Measurements of accessible chromatin are used extensively to identify genomic regulatory elements. Whilst chromatin landscapes of pluripotent stem cells are well characterised, chromatin accessibility changes in the development of somatic lineages are not well defined. Here we show that cell-specific chromatin accessibility data can be produced via ectopic expression of E. coli Dam methylase in vivo, without the requirement for cell-sorting (CATaDa). We have profiled chromatin accessibility in individual cell-types of Drosophila neural and midgut lineages. Functional cell-type specific enhancers were identified, as well as novel motifs enriched at different stages of development. Finally, we show global changes in the accessibility of chromatin between stem-cells and their differentiated progeny. Our results demonstrate the dynamic nature of chromatin accessibility in somatic tissues during stem cell differentiation and provide a novel approach to understanding gene regulatory mechanisms underlying development.
AU - Southall,TD
AU - Aughey,GN
AU - Estacio,Gomez A
AU - Yin,H
AU - Thomson,J
DO - 10.7554/eLife.32341
PY - 2018///
SN - 2050-084X
TI - CATaDa reveals global remodelling of chromatin accessibility during stem cell differentiation in vivo
T2 - eLife
UR - http://dx.doi.org/10.7554/eLife.32341
UR - http://hdl.handle.net/10044/1/56707
VL - 7
ER -