Publications
51 results found
Wolfram V, Southall TD, Guenay C, et al., 2014, The Transcription Factors Islet and Lim3 Combinatorially Regulate Ion Channel Gene Expression, JOURNAL OF NEUROSCIENCE, Vol: 34, Pages: 2538-2543, ISSN: 0270-6474
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- Citations: 19
Neville MC, Nojima T, Ashley E, et al., 2014, Male-Specific Fruitless Isoforms Target Neurodevelopmental Genes to Specify a Sexually Dimorphic Nervous System, Current Biology, Vol: 24, Pages: 229-241, ISSN: 0960-9822
Southall TD, Gold KS, Egger B, et al., 2013, Cell-Type-Specific Profiling of Gene Expression and Chromatin Binding without Cell Isolation: Assaying RNA Pol II Occupancy in Neural Stem Cells, DEVELOPMENTAL CELL, Vol: 26, Pages: 101-112, ISSN: 1534-5807
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- Citations: 142
Wolfram V, Southall TD, Brand AH, et al., 2012, The homeodomain transcription factors islet and lim3 synergize to regulate sh expression in motoneurons, JOURNAL OF NEUROGENETICS, Vol: 26, Pages: 36-36, ISSN: 0167-7063
Murray MJ, Southall TD, Liu W, et al., 2012, Snail-dependent repression of the RhoGEF <i>pebble</i> is required for gastrulation consistency in <i>Drosophila melanogaster</i>, DEVELOPMENT GENES AND EVOLUTION, Vol: 222, Pages: 361-368, ISSN: 0949-944X
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- Citations: 3
Wolfram V, Southall TD, Brand AH, et al., 2012, The LIM-Homeodomain Protein Islet Dictates Motor Neuron Electrical Properties by Regulating K<SUP>+</SUP> Channel Expression, NEURON, Vol: 75, Pages: 663-674, ISSN: 0896-6273
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- Citations: 29
Bardin AJ, Perdigoto CN, Southall TD, et al., 2010, Transcriptional control of stem cell maintenance in the <i>Drosophila</i> intestine, DEVELOPMENT, Vol: 137, Pages: 705-714, ISSN: 0950-1991
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- Citations: 129
Southall TD, Brand AH, 2009, Neural stem cell transcriptional networks highlight genes essential for nervous system development, EMBO JOURNAL, Vol: 28, Pages: 3799-3807, ISSN: 0261-4189
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- Citations: 90
Southall TD, Brand AH, 2008, Generation of Driver and Reporter Constructs for the GAL4 Expression System in Drosophila., CSH Protoc, Vol: 2008
INTRODUCTIONThe GAL4 system is a method for ectopic gene expression that allows the selective activation of any cloned gene in a wide variety of tissue- and cell-specific patterns. This protocol describes the generation of driver and reporter lines for use with the GAL4 system in Drosophila. A promoter-GAL4 fusion is constructed using a P-element transformable vector, and a GAL4-responsive target gene is created via generation of an upstream activation sequence (UAS)-reporter construct. An alternative strategy for integration using the phiC31 system is also provided. Transformant lines are generated using standard procedures for microinjection.
Southall TD, Elliott DA, Brand AH, 2008, The GAL4 System: A Versatile Toolkit for Gene Expression in Drosophila., CSH Protoc, Vol: 2008
INTRODUCTIONThe generation of gain-of-function phenotypes by ectopic expression of known genes provides a powerful complement to the genetic approach, in which genes are studied or identified through mutations that generally reduce or eliminate gene function. The GAL4 system is a method for ectopic gene expression that allows the selective activation of any cloned gene in a wide variety of tissue- and cell-specific patterns. A key advantage of the system is the separation of the GAL4 protein from its target gene in distinct transgenic lines, which ensures that the target gene is silent until the introduction of GAL4. Recent modifications and adaptations of the GAL4 system to make the system inducible have further expanded its scope, enabling greater temporal control over the activity of GAL4. There are now large resources for the community, including thousands of GAL4 lines and a wide selection of reporter lines. Here we present an overview of the GAL4 system, highlighting recent developments and discussing methods for generating and analyzing transgenic flies for GAL4-mediated ectopic expression.
Southall TD, Egger B, Gold KS, et al., 2008, Regulation of Self-renewal and Differentiation in the <i>Drosophila</i> Nervous System, 73rd Cold Spring Harbor Symposium on Quantitative Biology, Publisher: COLD SPRING HARBOR LABORATORY PRESS, Pages: 523-+, ISSN: 0091-7451
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- Citations: 8
Southall TD, Brand AH, 2007, Chromatin profiling in model organisms., Brief Funct Genomic Proteomic, Vol: 6, Pages: 133-140, ISSN: 1473-9550
The correct control of gene expression is essential for the proper development of organisms. Abnormal expression of genes can lead to cancerous growth and certain diseases. To understand how gene expression is controlled on a genome-wide scale, methods for assaying transcription factor binding sites are required. There are two prevailing techniques for mapping protein-chromatin interactions, ChIP (chromatin immunoprecipitation) and DamID (DNA adenine methyltransferase identification). Both of these methods, when combined with microarray technology, can provide powerful insights into transcription factor function, higher order chromatin structure and gene regulatory networks. In vivo chromatin profiling studies are now being performed on model organisms, targeting specific tissues to help generate more accurate maps of protein-DNA interactions.
Choksi SP, Southall TD, Bossing T, et al., 2006, Prospero acts as a binary switch between self-renewal and differentiation in <i>Drosophila</i> neural stem cells, DEVELOPMENTAL CELL, Vol: 11, Pages: 775-789, ISSN: 1534-5807
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- Citations: 290
Pym ECG, Southall TD, Mee CJ, et al., 2006, The homeobox transcription factor Even-skipped regulates acquisition of electrical properties in <i>Drosophila</i> neurons, NEURAL DEVELOPMENT, Vol: 1, ISSN: 1749-8104
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- Citations: 27
Terhzaz S, Southall TD, Lilley KS, et al., 2006, Differential gel electrophoresis and transgenic mitochondrial calcium reporters demonstrate spatiotemporal filtering in calcium control of mitochondria, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 281, Pages: 18849-18858, ISSN: 0021-9258
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- Citations: 45
Southall TD, Terhzaz S, Cabrero P, et al., 2006, Novel subcellular locations and functions for secretory pathway Ca<SUP>2+</SUP>/Mn<SUP>2+</SUP>-ATPases, PHYSIOLOGICAL GENOMICS, Vol: 26, Pages: 35-45, ISSN: 1094-8341
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- Citations: 39
Du J, Kean L, Allan AK, et al., 2006, The <i>SzA</i> mutations of the B subunit of the <i>Drosophila</i> vacuolar H<SUP>+</SUP> ATPase identify conserved residues essential for function in fly and yeast, JOURNAL OF CELL SCIENCE, Vol: 119, Pages: 2542-2551, ISSN: 0021-9533
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- Citations: 17
Torrie LS, Radford JC, Southall TD, et al., 2005, Resolution of the insect ouabain paradox, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, Vol: 141, Pages: S112-S112, ISSN: 1095-6433
Terhzaz S, Southall TD, Cabrero P, et al., 2005, SPoCk, a <i>Drosophila</i> Ca<SUP>2+</SUP>/Mn<SUP>2+</SUP> ATPase, plays multiple roles in epithelial calcium handling, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, Vol: 141, Pages: S111-S111, ISSN: 1095-6433
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- Citations: 2
MacPherson MR, Pollock VP, Kean L, et al., 2005, Transient receptor potential-like channels are essential for calcium signaling and fluid transport in a Drosophila epithelium, GENETICS, Vol: 169, Pages: 1541-1552, ISSN: 0016-6731
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- Citations: 25
Torrie LS, Radford JC, Southall TD, et al., 2004, Resolution of the insect ouabain paradox, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol: 101, Pages: 13689-13693, ISSN: 0027-8424
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- Citations: 114
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