Imperial College London
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Tony D. Southall

Faculty of Natural Sciences, Department of Life Sciences

Reader in Molecular Genetics
 
 
 
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Contact

 

+44 (0)20 7594 5338t.southall

 
 
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Location

 

407Sir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Cheetham:2018:10.1242/dev.170209,
author = {Cheetham, SW and Gruhn, WH and van, den Ameele J and Krautz, R and Southall, TD and Kobayashi, T and Surani, MA and Brand, AH},
doi = {10.1242/dev.170209},
journal = {Development},
title = {Targeted DamID reveals differential binding of mammalian pluripotency factors},
url = {http://dx.doi.org/10.1242/dev.170209},
volume = {145},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The precise control of gene expression by transcription factor networks is crucial to organismal development. The predominant approach for mapping transcription factor-chromatin interactions has been chromatin immunoprecipitation (ChIP). However, ChIP requires a large number of homogeneous cells and antisera with high specificity. A second approach, DamID, has the drawback that high levels of Dam methylase are toxic. Here, we modify our targeted DamID approach (TaDa) to enable cell type-specific expression in mammalian systems, generating an inducible system (mammalian TaDa or MaTaDa) to identify genome-wide protein/DNA interactions in 100 to 1000 times fewer cells than ChIP-based approaches. We mapped the binding sites of two key pluripotency factors, OCT4 and PRDM14, in mouse embryonic stem cells, epiblast-like cells and primordial germ cell-like cells (PGCLCs). PGCLCs are an important system for elucidating primordial germ cell development in mice. We monitored PRDM14 binding during the specification of PGCLCs, identifying direct targets of PRDM14 that are key to understanding its crucial role in PGCLC development. We show that MaTaDa is a sensitive and accurate method for assessing cell type-specific transcription factor binding in limited numbers of cells.
AU  - Cheetham,SW
AU  - Gruhn,WH
AU  - van,den Ameele J
AU  - Krautz,R
AU  - Southall,TD
AU  - Kobayashi,T
AU  - Surani,MA
AU  - Brand,AH
DO  - 10.1242/dev.170209
PY  - 2018///
SN  - 0950-1991
TI  - Targeted DamID reveals differential binding of mammalian pluripotency factors
T2  - Development
UR  - http://dx.doi.org/10.1242/dev.170209
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000449152600014&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/67190
VL  - 145
ER  -
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