Imperial College London
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Tony D. Southall

Faculty of Natural Sciences, Department of Life Sciences

Reader in Molecular Genetics
 
 
 
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Contact

 

+44 (0)20 7594 5338t.southall

 
 
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Location

 

407Sir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@unpublished{Aughey:2017:10.1101/189456,
author = {Aughey, GN and Estacio, Gomez A and Thomson, J and Yin, H and Southall, TD},
doi = {10.1101/189456},
title = {CATaDa reveals global remodelling of chromatin accessibility during stem cell differentiation in vivo},
url = {http://dx.doi.org/10.1101/189456},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - UNPB
AB  - <jats:title>Abstract</jats:title><jats:p>Regulation of eukaryotic gene expression is coordinated by dynamic changes to chromatin states throughout development. Measurements of accessible chromatin are used extensively to identify genomic regulatory elements. Whilst the chromatin landscapes of pluripotent stem cells are well characterised, chromatin accessibility changes in the development of somatic stem cell lineages are not well defined. Here we show that tissue specific chromatin accessibility data can be produced via ectopic expression of<jats:italic>E. coli</jats:italic>Dam methylase<jats:italic>in vivo</jats:italic>, without the requirement for cell-sorting. We have profiled chromatin accessibility in individual cell types of the<jats:italic>Drosophila</jats:italic>neural and midgut stem cell lineages. Functional cell-type specific enhancers were identified, as well as novel motifs enriched at diferent stages of development. Finally, we show global changes in the accessibility of chromatin between stem-cells and their diferentiated progeny. Our results demonstrate the dynamic nature of chromatin accessibility in somatic tissues during stem cell diferentiation and provide a novel approach to understanding the gene regulatory mechanisms underlying development.</jats:p>
AU  - Aughey,GN
AU  - Estacio,Gomez A
AU  - Thomson,J
AU  - Yin,H
AU  - Southall,TD
DO  - 10.1101/189456
PY  - 2017///
TI  - CATaDa reveals global remodelling of chromatin accessibility during stem cell differentiation in vivo
UR  - http://dx.doi.org/10.1101/189456
ER  -
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