Imperial College London
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Tony D. Southall

Faculty of Natural Sciences, Department of Life Sciences

Reader in Molecular Genetics
 
 
 
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Contact

 

+44 (0)20 7594 5338t.southall

 
 
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Location

 

407Sir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Dinges:2017:10.1016/j.celrep.2017.11.015,
author = {Dinges, N and Morin, V and Kreim, N and Southall, TD and Roignant, J-Y},
doi = {10.1016/j.celrep.2017.11.015},
journal = {Cell Reports},
pages = {2911--2925},
title = {Comprehensive Characterization of the Complex lola Locus Reveals a Novel Role in the Octopaminergic Pathway via Tyramine Beta-Hydroxylase Regulation},
url = {http://dx.doi.org/10.1016/j.celrep.2017.11.015},
volume = {21},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Longitudinals lacking (lola) is one of the most complex genes in Drosophila melanogaster, encoding up to 20 protein isoforms that include key transcription factors involved in axonal pathfinding and neural reprogramming. Most previous studies have employed loss-of-function alleles that disrupt lola common exons, making it difficult to delineate isoform-specific functions. To overcome this issue, we have generated isoform-specific mutants for all isoforms using CRISPR/Cas9. This enabled us to study specific isoforms with respect to previously characterized roles for Lola and to demonstrate a specific function for one variant in axon guidance via activation of the microtubule-associated factor Futsch. Importantly, we also reveal a role for a second variant in preventing neurodegeneration via the positive regulation of a key enzyme of the octopaminergic pathway. Thus, our comprehensive study expands the functional repertoire of Lola functions, and it adds insights into the regulatory control of neurotransmitter expression in vivo.
AU  - Dinges,N
AU  - Morin,V
AU  - Kreim,N
AU  - Southall,TD
AU  - Roignant,J-Y
DO  - 10.1016/j.celrep.2017.11.015
EP  - 2925
PY  - 2017///
SN  - 2211-1247
SP  - 2911
TI  - Comprehensive Characterization of the Complex lola Locus Reveals a Novel Role in the Octopaminergic Pathway via Tyramine Beta-Hydroxylase Regulation
T2  - Cell Reports
UR  - http://dx.doi.org/10.1016/j.celrep.2017.11.015
UR  - http://hdl.handle.net/10044/1/55780
VL  - 21
ER  -
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