179 results found
Jarvis IWH, Enlo-Scott Z, Nagy E, et al., 2018, Genotoxicity of fine and coarse fraction ambient particulate matter in immortalised normal (TT1) and cancer-derived (A549) alveolar epithelial cells., Environ Mol Mutagen
Human exposure to airborne particulate matter (PM) is associated with adverse cardiopulmonary health effects, including lung cancer. Ambient PM represents a heterogeneous mixture of chemical classes including transition metals, polycyclic aromatic hydrocarbons (PAHs) and their derivatives such as nitro-PAHs, many of which are classified as putative carcinogens. As the primary site of human exposure to PM is the lungs, we investigated the response of two alveolar epithelial cell lines, the tumour-derived A549 and newly described TT1 cells, to fine and coarse PM collected from background and roadside locations. We show that coarse PM elicits a genotoxic response in the TT1 cells, with the strongest signal associated with the background sample. This response could be recapitulated using the organic extract derived from this sample. No responses were observed in PM-challenged A549 cells. Fine PM failed to elicit a genotoxic response in either cell line despite the higher PAH concentrations within this fraction. Consistent with the lack of a simplistic association between PM PAH content and the observed genotoxic response, TT1 cells treated with benzo[a]pyrene (BaP) demonstrated no increase in the selected markers. In contrast, a pattern of response was observed in TT1 cells challenged with 3-nitrobenzanthrone (3-NBA) similar to that with coarse PM. Together, these data illustrated the suitability of the TT1 cell line for assessing PM-induced genotoxicity and challenge the contention that fine roadside PM poses the higher cancer risk. Furthermore, the response to 3-NBA and not BaP suggests a major contribution of nitro-PAHs to the overall toxicity of PM. Environ. Mol. Mutagen., 2018. © 2018 Wiley Periodicals, Inc.
Katsumiti A, Thorley AJ, Arostegui I, et al., 2018, Cytotoxicity and cellular mechanisms of toxicity of CuO NPs in mussel cells in vitro and comparative sensitivity with human cells., Toxicol In Vitro, Vol: 48, Pages: 146-158
There is a need to assess human and ecosystem health effects of copper oxide nanoparticles (CuO NPs), extensively used in many industrial products. Here, we aimed to determine the cytotoxicity and cellular mechanisms involved in the toxicity of CuO NPs in mussel cells (hemocytes and gill cells) in parallel with exposures to ionic Cu and bulk CuO, and to compare the sensitivity of mussel primary cells with a well-established human cell line (pulmonary TT1 cells). At similar doses, CuO NPs promoted dose-dependent cytotoxicity and increased reactive oxygen species (ROS) production in mussel and human cells. In mussel cells, ionic Cu was more toxic than CuO NPs and the latter more than bulk CuO. Ionic Cu and CuO NPs increased catalase and acid phosphatase activities in both mussel cells and decreased gill cells Na-K-ATPase activity. All Cu forms produced DNA damage in hemocytes, whereas in gill cells only ionic Cu and CuO NPs were genotoxic. Induction of the MXR transport activity was found in gill cells exposed to all forms of Cu and in hemocytes exposed to ionic Cu and CuO NPs. Phagocytosis increased only in hemocytes exposed to CuO NPs, indicating a nanoparticle-specific immunostimulatory effect. In conclusion, toxicity of CuO NPs is driven by ROS in human and mussel cells. Mussel cells respond to CuO NP exposure by triggering an array of defensive mechanisms.
Calderon L, Han TT, McGilvery CM, et al., 2017, Release of airborne particles and Ag and Zn compounds from nanotechnology-enabled consumer sprays: Implications for inhalation exposure, ATMOSPHERIC ENVIRONMENT, Vol: 155, Pages: 85-96, ISSN: 1352-2310
Chung KF, Seiffert J, Chen S, et al., 2017, Inactivation, Clearance, and Functional Effects of Lung-Instilled Short and Long Silver Nanowires in Rats, ACS NANO, Vol: 11, Pages: 2652-2664, ISSN: 1936-0851
Conforti F, Davies ER, Calderwood CJ, et al., 2017, The histone deacetylase inhibitor, romidepsin, as a potential treatment for pulmonary fibrosis, ONCOTARGET, Vol: 8, Pages: 48737-48754, ISSN: 1949-2553
Mohamed NA, Davies RP, Lickiss PD, et al., 2017, Chemical and biological assessment of metal organic frameworks (MOFs) in pulmonary cells and in an acute in vivo model: relevance to pulmonary arterial hypertension therapy, PULMONARY CIRCULATION, Vol: 7, Pages: 643-653, ISSN: 2045-8932
Robinson RK, Birrell MA, Adcock JJ, et al., 2017, Mechanistic link between diesel exhaust particles and respiratory reflexes., J Allergy Clin Immunol
BACKGROUND: Diesel exhaust particles (DEPs) are a major component of particulate matter in Europe's largest cities, and epidemiologic evidence links exposure with respiratory symptoms and asthma exacerbations. Respiratory reflexes are responsible for symptoms and are regulated by vagal afferent nerves, which innervate the airway. It is not known how DEP exposure activates airway afferents to elicit symptoms, such as cough and bronchospasm. OBJECTIVE: We sought to identify the mechanisms involved in activation of airway sensory afferents by DEPs. METHODS: In this study we use in vitro and in vivo electrophysiologic techniques, including a unique model that assesses depolarization (a marker of sensory nerve activation) of human vagus. RESULTS: We demonstrate a direct interaction between DEP and airway C-fiber afferents. In anesthetized guinea pigs intratracheal administration of DEPs activated airway C-fibers. The organic extract (DEP-OE) and not the cleaned particles evoked depolarization of guinea pig and human vagus, and this was inhibited by a transient receptor potential ankyrin-1 antagonist and the antioxidant N-acetyl cysteine. Polycyclic aromatic hydrocarbons, major constituents of DEPs, were implicated in this process through activation of the aryl hydrocarbon receptor and subsequent mitochondrial reactive oxygen species production, which is known to activate transient receptor potential ankyrin-1 on nociceptive C-fibers. CONCLUSIONS: This study provides the first mechanistic insights into how exposure to urban air pollution leads to activation of guinea pig and human sensory nerves, which are responsible for respiratory symptoms. Mechanistic information will enable the development of appropriate therapeutic interventions and mitigation strategies for those susceptible subjects who are most at risk.
Smyth E, Solomon A, Birrell MA, et al., 2017, Influence of inflammation and nitric oxide upon platelet aggregation following deposition of diesel exhaust particles in the airways, BRITISH JOURNAL OF PHARMACOLOGY, Vol: 174, Pages: 2130-2139, ISSN: 0007-1188
Smyth E, Solomon A, Birrell MA, et al., 2017, Response to 'Effects of diesel exhaust particles on coagulation', BRITISH JOURNAL OF PHARMACOLOGY, Vol: 174, Pages: 4200-4200, ISSN: 0007-1188
Thorley A, Ogger P, Legorburo-Schofield M, et al., 2017, Nintedanib Effectively Inhibits Carbon Nanotube-Induced Fibrotic Responses In Human Alveolar Epithelial Cells, Fibroblasts And Pulmonary Microvascular Endothelial Cells, International Conference of the American-Thoracic-Society (ATS), Publisher: AMER THORACIC SOC, ISSN: 1073-449X
Botelho DJ, Leo BF, Massa CB, et al., 2016, Low-dose AgNPs reduce lung mechanical function and innate immune defense in the absence of cellular toxicity, NANOTOXICOLOGY, Vol: 10, Pages: 118-127, ISSN: 1743-5390
Chen S, Goode AE, Skepper JN, et al., 2016, Avoiding artefacts during electron microscopy of silver nanomaterials exposed to biological environments, JOURNAL OF MICROSCOPY, Vol: 261, Pages: 157-166, ISSN: 0022-2720
Robinson RK, Birrell MA, Wortley MA, et al., 2016, Diesel Activates Airway Sensory Nerves To Initiate Respiratory Symptoms, International Conference of the American-Thoracic-Society (ATS), Publisher: AMER THORACIC SOC, ISSN: 1073-449X
Ruenraromgsak P, Chen S, Hu S, et al., 2016, Translocation of Functionalized Multi-Walled Carbon Nanotubes across Human Pulmonary Alveolar Epithelium: Dominant Role of Epithelial Type 1 Cells, ACS NANO, Vol: 10, Pages: 5070-5085, ISSN: 1936-0851
Sarkar S, Carranza C, Theodorou I, et al., 2016, Impact of Silver and Carbon Nanoparticle Exposures on Macrophage Responses to Mycobacterium tuberculosis (M.tb), Annual Meeting of the American-Association-of-Immunologists (AAI), Publisher: AMER ASSOC IMMUNOLOGISTS, ISSN: 0022-1767
Seiffert J, Buckley A, Leo B, et al., 2016, Pulmonary effects of inhalation of spark-generated silver nanoparticles in Brown-Norway and Sprague-Dawley rats, RESPIRATORY RESEARCH, Vol: 17, ISSN: 1465-993X
Serrano-Mollar A, Gay-Jordi G, Guillamat-Prats R, et al., 2016, Safety and Tolerability of Alveolar Type II Cell Transplantation in Idiopathic Pulmonary Fibrosis, CHEST, Vol: 150, Pages: 533-543, ISSN: 0012-3692
Sweeney S, Hu S, Ruenraroengsak P, et al., 2016, Carboxylation of multiwalled carbon nanotubes reduces their toxicity in primary human alveolar macrophages, ENVIRONMENTAL SCIENCE-NANO, Vol: 3, Pages: 1340-1350, ISSN: 2051-8153
Sweeney S, Leo BF, Chen S, et al., 2016, Pulmonary surfactant mitigates silver nanoparticle toxicity in human alveolar type-I-like epithelial cells, COLLOIDS AND SURFACES B-BIOINTERFACES, Vol: 145, Pages: 167-175, ISSN: 0927-7765
Theodorou IG, Ruenraroengsak P, Gow A, et al., 2016, Effect of pulmonary surfactant on the dissolution, stability and uptake of zinc oxide nanowires by human respiratory epithelial cells, NANOTOXICOLOGY, Vol: 10, Pages: 1351-1362, ISSN: 1743-5390
Zhang JJ, Lee K-B, He L, et al., 2016, Effects of a nanoceria fuel additive on the physicochemical properties of diesel exhaust particles, ENVIRONMENTAL SCIENCE-PROCESSES & IMPACTS, Vol: 18, Pages: 1333-1342, ISSN: 2050-7887
Botelho D, Shaffer M, Porter A, et al., 2015, Lung Lining Interaction Determines the Fate of Multi-Walled Carbon Nanotubes (MWCNTs) in vivo, Experimental Biology Meeting, Publisher: FEDERATION AMER SOC EXP BIOL, ISSN: 0892-6638
Cryer AM, Ruenraroengsak P, Tetley TD, et al., 2015, SYNTHESIS OF GOLD-BASED NANOMEDICINES TO TREAT NON-SMALL CELL LUNG CANCER, Winter Meeting of the British-Thoracic-Society, Publisher: BMJ PUBLISHING GROUP, Pages: A58-A59, ISSN: 0040-6376
Marchetti M, Shaffer MSP, Zambianchi M, et al., 2015, Adsorption of surfactant protein D from human respiratory secretions by carbon nanotubes and polystyrene nanoparticles depends on nanomaterial surface modification and size, PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, Vol: 370, ISSN: 0962-8436
McKenzie Z, Kendall M, Mackay R-M, et al., 2015, Nanoparticles modulate surfactant protein A and D mediated protection against influenza A infection in vitro, PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, Vol: 370, ISSN: 0962-8436
Melbourne J, Clancy A, Seiffert J, et al., 2015, An investigation of the carbon nanotube - Lipid interface and its impact upon pulmonary surfactant lipid function., Biomaterials, Vol: 55, Pages: 24-32, ISSN: 1878-5905
Multiwalled carbon nanotubes (MWCNTs) are now synthesized on a large scale, increasing the risk of occupational inhalation. However, little is known of the MWCNT-pulmonary surfactant (PS) interface and its effect on PS functionality. The Langmuir-Blodgett trough was used to evaluate the impact of MWCNTs on fundamental properties of PS lipids which influence PS function, i.e. compression resistance and maximum obtainable pressure. Changes were found to be MWCNT length-dependent. 'Short' MWCNTs (1.1 μm, SD = 0.61) penetrated the lipid film, reducing the maximum interfacial film pressure by 10 mN/m (14%) in dipalmitoylphosphatidylcholine (DPPC) and PS, at an interfacial MWCNT-PS lipid mass ratio range of 50:1 to 1:1. 'Long' commercial MWCNTs (2.1 μm, SD = 1.2) caused compression resistance at the same mass loadings. 'Very long' MWCNTs (35 μm, SD = 19) sequestered DPPC and were squeezed out of the DPPC film. High resolution transmission electron microscopy revealed that all MWCNT morphologies formed DPPC coronas with ordered arrangements. These results provide insight into how nanoparticle aspect ratio affects the interaction mechanisms with PS, in its near-native state at the air-water interface.
Mukherjee D, Porter A, Ryan M, et al., 2015, Modeling In Vivo Interactions of Engineered Nanoparticles in the Pulmonary Alveolar Lining Fluid, NANOMATERIALS, Vol: 5, Pages: 1223-1249, ISSN: 2079-4991
Nyga A, Hart A, Tetley TD, 2015, Importance of the HIF pathway in cobalt nanoparticle-induced cytotoxicity and inflammation in human macrophages, NANOTOXICOLOGY, Vol: 9, Pages: 905-917, ISSN: 1743-5390
Ruenraroengsak P, Tetley TD, 2015, Differential bioreactivity of neutral, cationic and anionic polystyrene nanoparticles with cells from the human alveolar compartment: robust response of alveolar type 1 epithelial cells, PARTICLE AND FIBRE TOXICOLOGY, Vol: 12, ISSN: 1743-8977
Sarkar S, Leo BF, Carranza C, et al., 2015, Modulation of Human Macrophage Responses to Mycobacterium tuberculosis by Silver Nanoparticles of Different Size and Surface Modification, PLOS ONE, Vol: 10, ISSN: 1932-6203
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