Publications
84 results found
Braga V, 2000, The crossroads between cell-cell adhesion and motility., Nat Cell Biol, Vol: 2, Pages: E182-E184, ISSN: 1465-7392
Espada J, Pérez-Moreno M, Braga VM, et al., 1999, H-Ras activation promotes cytoplasmic accumulation and phosphoinositide 3-OH kinase association of beta-catenin in epidermal keratinocytes., Journal of Cell Biology, Vol: 146, Pages: 967-980, ISSN: 0021-9525
The mechanisms underlying downregulation of the cadherin/catenin complexes and beta-catenin signaling during tumor progression are not fully understood. We have analyzed the effect of oncogenic H-Ras on E-cadherin/catenin complex formation/stabilization and beta-catenin distribution in epidermal keratinocytes. Microinjection or stable expression of V12Ras into keratinocytes promotes the loss of E-cadherin and alpha-catenin and relocalization of beta-catenin to the cytoplasm and nucleus. Moreover, these effects are dependent on PI3K (phosphoinositide 3-OH kinase) activity. Interestingly, a strong association of p85alpha and p110alpha subunits of PI3K with beta-catenin is induced in V12Ras-expressing keratinocytes, and in vitro binding assays show a direct interaction between beta-catenin and p85alpha. Overexpression of either V12Ras or constitutively active p110alpha induces metabolic stabilization of beta-catenin and promotes its accumulation in cytoplasmic and nuclear pools. In addition, the interaction of beta-catenin with the adenomatous polyposis coli protein is blocked in V12Ras and p110alpha transformants though no changes in glycogen synthase kinase 3 beta activity could be detected. Nevertheless, in V12Ras transformants the in vivo phosphorylation of beta-catenin in Ser residues is strongly decreased. These results indicate that H-Ras activation induces the relocalization and cytoplasmic stabilization of beta-catenin by a mechanism involving its interaction with PI3K.
Braga VMM, 1999, Small GTPases and regulation of cadherin dependent cell-cell adhesion, JOURNAL OF CLINICAL PATHOLOGY-MOLECULAR PATHOLOGY, Vol: 52, Pages: 197-202, ISSN: 1366-8714
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- Citations: 37
Weil M, Raff MC, Braga VMM, 1999, Caspase activation in the terminal differentiation of human epidermal keratinocytes, CURRENT BIOLOGY, Vol: 9, Pages: 361-364, ISSN: 0960-9822
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- Citations: 173
Braga VMM, Del Maschio A, Machesky L, et al., 1999, Regulation of cadherin function by Rho and Rac: Modulation by junction maturation and cellular context, MOLECULAR BIOLOGY OF THE CELL, Vol: 10, Pages: 9-22, ISSN: 1059-1524
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- Citations: 215
Braga VMM, Hajbagheri N, Watt FM, 1998, Calcium-induced intercellular adhesion of keratinocytes does not involve accumulation of β<sub>1</sub> integrins at cell-cell contacts and does not involve changes in the levels or phosphorylation of catenins, CELL ADHESION AND COMMUNICATION, Vol: 5, Pages: 137-+, ISSN: 1061-5385
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- Citations: 13
Braga VM, Machesky LM, Hall A, et al., 1997, The small GTPases Rho and Rac are required for the establishment of cadherin-dependent cell-cell contacts., Journal of Cell Biology, Vol: 137, Pages: 1421-1431, ISSN: 0021-9525
Cadherins are calcium-dependent cell-cell adhesion molecules that require the interaction of the cytoplasmic tail with the actin cytoskeleton for adhesive activity. Because of the functional relationship between cadherin receptors and actin filament organization, we investigated whether members of the Rho family of small GTPases are necessary for cadherin adhesion. In fibroblasts, the Rho family members Rho and Rac regulate actin polymerization to produce stress fibers and lamellipodia, respectively. In epithelial cells, we demonstrate that Rho and Rac are required for the establishment of cadherin-mediated cell-cell adhesion and the actin reorganization necessary to stabilize the receptors at sites of intercellular junctions. Blocking endogenous Rho or Rac selectively removed cadherin complexes from junctions induced for up to 3 h, while desmosomes were not perturbed. In addition, withdrawal of cadherins from intercellular junctions temporally precedes the removal of CD44 and integrins, other microfilament-associated receptors. Our data showed that the concerted action of Rho and Rac modulate the establishment of cadherin adhesion: a constitutively active form of Rac was not sufficient to stabilize cadherindependent cell-cell contacts when endogenous Rho was inhibited. Upon induction of calcium-dependent intercellular adhesion, there was a rapid accumulation of actin at sites of cell-cell contacts, which was prevented by blocking cadherin function, Rho or Rac activity. However, if cadherin complexes are clustered by specific antibodies attached to beads, actin recruitment to the receptors was perturbed by inhibiting Rac but not Rho. Our results provide new insights into the role of the small GTPases in the cadherin-dependent cell- cell contact formation and the remodelling of actin filaments in epithelial cells.
GIANNINI ALM, LINHARES SV, CARIDE EC, et al., 1995, MOLECULAR ASPECTS OF SCHISTOSOMA-MANSONI FEMALE MATURATION, MEMORIAS DO INSTITUTO OSWALDO CRUZ, Vol: 90, Pages: 179-184, ISSN: 0074-0276
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- Citations: 2
GIANNINI ALM, CARIDE EC, BRAGA VMM, et al., 1995, F-10 NUCLEAR-BINDING PROTEINS OF SCHISTOSOMA-MANSONI - STRUCTURAL AND FUNCTIONAL FEATURES, PARASITOLOGY, Vol: 110, Pages: 155-161, ISSN: 0031-1820
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- Citations: 9
BRAGA VMM, HODIVALA KJ, WATT FM, 1995, CALCIUM-INDUCED CHANGES IN DISTRIBUTION AND SOLUBILITY OF CADHERINS, INTEGRINS AND THEIR ASSOCIATED CYTOPLASMIC PROTEINS IN HUMAN KERATINOCYTES, CELL ADHESION AND COMMUNICATION, Vol: 3, Pages: 201-215, ISSN: 1061-5385
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- Citations: 56
BRAGA VMM, GENDLER SJ, 1994, COAMPLIFICATION OF 2 CDNAS IN RT-PCR CAN ALTER THE YIELD OF BOTH PRODUCTS, BIOTECHNIQUES, Vol: 17, Pages: 228-230, ISSN: 0736-6205
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- Citations: 13
BRAGA VMM, GENDLER SJ, 1993, MODULATION OF MUC-1 MUCIN EXPRESSION IN THE MOUSE UTERUS DURING THE ESTRUS CYCLE, EARLY-PREGNANCY AND PLACENTATION, JOURNAL OF CELL SCIENCE, Vol: 105, Pages: 397-405, ISSN: 0021-9533
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- Citations: 128
Braga VM, Pemberton L, Duhig T, et al., 1992, Spatial and temporal expression of an epithelial mucin, Muc-1, during mouse development, Development, Pages: 427-437
PENA SDJ, MACEDO AM, BRAGA VMM, et al., 1990, F10, THE GENE FOR THE GLYCINE-RICH MAJOR EGGSHELL PROTEIN OF SCHISTOSOMA-MANSONI RECOGNIZES A FAMILY OF HYPERVARIABLE MINISATELLITES IN THE HUMAN GENOME, NUCLEIC ACIDS RESEARCH, Vol: 18, Pages: 7466-7466, ISSN: 0305-1048
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- Citations: 12
Smithers SR, Hackett F, Braga V, et al., 1990, Immunoblotting identifies additional antigens recognised by mice protectively vaccinated with adultSchistosoma mansoni tegumental membranes, Parasitology Research, Vol: 76, Pages: 454-456, ISSN: 0044-3255
Braga VM, Pena SDJ, Macedo AM, et al., 1990, Spatial and temporal expression of an epithelial mucin, Muc-1, during mouse development., Nucleic Acid Res., Vol: 24
Braga VM, Pena SDJ, Macedo AM, et al., 1990, The nucleotide sequence encoding the glycine-rich major egg-shell protein of Schistosoma mansoni recognizes a family of hypervariable minisatellites in the human genome., Progress in Nucleic Acid Research and Molecular Biology, Vol: 24
RUMJANEK FD, BRAGA VMM, KELLY C, 1989, DNA-BINDING PROTEINS OF SCHISTOSOMA-MANSONI RECOGNIZING A HEXANUCLEOTIDE MOTIF OCCURRING IN GENES REGULATED BY STEROIDS, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, Vol: 94, Pages: 807-812, ISSN: 0305-0491
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- Citations: 9
Braga VM, Kelly C, Rumjanek FD, 1989, DNA binding proteins of Schistosoma mansoni: recognition of a hexanucleotide motif for binding of steroids receptors, Comp. Biochem. Physiol., Vol: 94, Pages: 807-812
BRAGA VMM, TAVARES CAP, RUMJANEK FD, 1989, PROTEIN CHARACTERIZATION OF SEXUALLY MATURE AND IMMATURE FORMS OF SCHISTOSOMA-MANSONI, COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, Vol: 94, Pages: 427-433, ISSN: 1096-4959
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- Citations: 5
Braga VM, Nicoli JR, 1988, Submerged and solid state fermentations of a Napier grass (Pennisetum purpureum Schumaker) residue by Phanerochete chrysosporium, Rev. Latinoamer. Microbiol, Vol: 4, Pages: 357-361
Rumjanek FD, Braga VM, Silveira AM, et al., 1987, Genomic regulation in immature females of Schistosoma mansoni., Mem Inst Oswaldo Cruz, Vol: 82 Suppl 4, Pages: 209-211, ISSN: 0074-0276
Braga VM, 1987, Influence of spore and mycelium inoculation of Phanerochaete chrysosporium on solid state fermentation of Napier grass (Pennisetum purpureum Schumaker) residue, Rev. Microbiol, Pages: 56-58
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