Imperial College London
Alternate

ProfessorVaniaBraga

Faculty of Medicine, National Heart & Lung Institute

Professor of Cellular Signalling
 
 
 
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Contact

 

+44 (0)20 7594 3233v.braga

 
 
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Location

 

Office no. 105Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Tzircotis:2011:10.1242/jcs.084269,
author = {Tzircotis, G and Braga, VMM and Caron, E},
doi = {10.1242/jcs.084269},
journal = {JOURNAL OF CELL SCIENCE},
pages = {2897--2902},
title = {RhoG is required for both Fc gamma R- and CR3-mediated phagocytosis},
url = {http://dx.doi.org/10.1242/jcs.084269},
volume = {124},
year = {2011}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Phagocytosis is a highly ordered process orchestrated by signalling through Rho GTPases to locally organise the actin cytoskeleton and drive particle uptake. Specific Rho family members that regulate phagocytosis are not known, as the majority of studies have relied on the use of dominant-negative mutants and/or toxins, which can inactivate multiple Rho GTPases. To identify the relevant GTPases for phagocytosis through the Fcγ receptor (FcγR) and complement receptor 3 (CR3), we depleted 20 Rho proteins individually in an RNA interference (RNAi) screen. We find that distinct GTPase subsets are required for actin polymerisation and uptake by macrophages: FcγR-dependent engulfment requires Cdc42 and Rac2 (but not Rac1), whereas CR3 requires RhoA. Surprisingly, RhoG is required for particle uptake through both FcγR and CR3. RhoG has been previously linked to Rac and Cdc42 signalling in different model systems, but not to RhoA. Interestingly, we find that RhoG is also recruited and activated at phagocytic cups downstream of FcγR and CR3, irrespective of their distinct actin structures and mechanisms of internalisation. Thus, the functional links between RhoG and RhoA downstream of CR3-dependent phagocytosis are new and unexpected. Our data suggest a broad role for RhoG in consolidating signals from multiple receptors during phagocytosis.
AU  - Tzircotis,G
AU  - Braga,VMM
AU  - Caron,E
DO  - 10.1242/jcs.084269
EP  - 2902
PY  - 2011///
SN  - 0021-9533
SP  - 2897
TI  - RhoG is required for both Fc gamma R- and CR3-mediated phagocytosis
T2  - JOURNAL OF CELL SCIENCE
UR  - http://dx.doi.org/10.1242/jcs.084269
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000294419200003&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/62122
VL  - 124
ER  -
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