Imperial College London
Alternate

ProfessorVaniaBraga

Faculty of Medicine, National Heart & Lung Institute

Professor of Cellular Signalling
 
 
 
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Contact

 

+44 (0)20 7594 3233v.braga

 
 
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Location

 

Office no. 105Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Nola:2012:10.1007/978-1-61779-442-1_10,
author = {Nola, S and Erasmus, JC and Braga, VM},
doi = {10.1007/978-1-61779-442-1_10},
pages = {143--155},
title = {Quantitative and robust assay to measure cell-cell contact assembly and maintenance},
url = {http://dx.doi.org/10.1007/978-1-61779-442-1_10},
volume = {827},
year = {2012}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Epithelial junction formation and maintenance are multistep processes that rely on the clustering of macromolecular complexes. These events are highly regulated by signalling pathways that involve Rho small GTPases. Usually, when analysing the contribution of different components of Rho-dependent pathways to cell-cell adhesion, the localisation of adhesion receptors at junctions is evaluated by immunofluorescence. However, we find that this method has limitations on the quantification (dynamic range), ability to detect partial phenotypes and to differentiate between the participation of a given regulatory protein in assembly and/or maintenance of cell-cell contacts.In this chapter, we describe a suitable method, the aggregation assay, in which we adapted a quantitative strategy to allow objective and reproducible detection of partial phenotypes. Importantly, this methodology estimates the ability of cells to form junctions and their resistance to mechanical shearing forces (stabilisation).
AU  - Nola,S
AU  - Erasmus,JC
AU  - Braga,VM
DO  - 10.1007/978-1-61779-442-1_10
EP  - 155
PY  - 2012///
SN  - 1940-6029
SP  - 143
TI  - Quantitative and robust assay to measure cell-cell contact assembly and maintenance
UR  - http://dx.doi.org/10.1007/978-1-61779-442-1_10
UR  - http://www.ncbi.nlm.nih.gov/pubmed/22144273
VL  - 827
ER  -
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