Imperial College London
Alternate

ProfessorVaniaBraga

Faculty of Medicine, National Heart & Lung Institute

Professor of Cellular Signalling
 
 
 
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Contact

 

+44 (0)20 7594 3233v.braga

 
 
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Location

 

Office no. 105Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Carroll:2013:10.1016/j.devcel.2013.03.005,
author = {Carroll, B and Mohd-Naim, N and Maximiano, F and Frasa, MA and McCormack, J and Finelli, M and Thoresen, SB and Perdios, L and Daigaku, R and Francis, RE and Futter, C and Dikic, I and Braga, VMM},
doi = {10.1016/j.devcel.2013.03.005},
journal = {Developmental Cell},
pages = {15--28},
title = {The TBC/RabGAP Armus Coordinates Rac1 and Rab7 Functions during Autophagy},
url = {http://dx.doi.org/10.1016/j.devcel.2013.03.005},
volume = {25},
year = {2013}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Autophagy is an evolutionarily conserved process that enables catabolic and degradative pathways. These pathways commonly depend on vesicular transport controlled by Rabs, small GTPases inactivated by TBC/RabGAPs. The Rac1 effector TBC/RabGAP Armus (TBC1D2A) is known to inhibit Rab7, a key regulator of lysosomal function. However, the precise coordination of signaling and intracellular trafficking that regulates autophagy is poorly understood. We find that overexpression of Armus induces the accumulation of enlarged autophagosomes, while Armus depletion significantly delays autophagic flux. Upon starvation-induced autophagy, Rab7 is transiently activated. This spatiotemporal regulation of Rab7 guanosine triphosphate/guanosine diphosphate cycling occurs by Armus recruitment to autophagosomes via interaction with LC3, a core autophagy regulator. Interestingly, autophagy potently inactivates Rac1. Active Rac1 competes with LC3 for interaction with Armus and thus prevents its appropriate recruitment to autophagosomes. The precise coordination between Rac1 and Rab7 activities during starvation suggests that Armus integrates autophagy with signaling and endocytic trafficking.
AU  - Carroll,B
AU  - Mohd-Naim,N
AU  - Maximiano,F
AU  - Frasa,MA
AU  - McCormack,J
AU  - Finelli,M
AU  - Thoresen,SB
AU  - Perdios,L
AU  - Daigaku,R
AU  - Francis,RE
AU  - Futter,C
AU  - Dikic,I
AU  - Braga,VMM
DO  - 10.1016/j.devcel.2013.03.005
EP  - 28
PY  - 2013///
SN  - 1878-1551
SP  - 15
TI  - The TBC/RabGAP Armus Coordinates Rac1 and Rab7 Functions during Autophagy
T2  - Developmental Cell
UR  - http://dx.doi.org/10.1016/j.devcel.2013.03.005
UR  - http://hdl.handle.net/10044/1/41496
VL  - 25
ER  -
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