Imperial College London

DrVladimirPelicic

Faculty of MedicineDepartment of Medicine

Reader in Molecular Bacteriology & Infection
 
 
 
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Contact

 

+44 (0)20 7594 2080v.pelicic Website

 
 
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Location

 

3.20Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Rusniok:2009:10.1186/gb-2009-10-10-r110,
author = {Rusniok, C and Vallenet, D and Floquet, S and Ewles, H and Mouzé-Soulama, C and Brown, D and Lajus, A and Buchrieser, C and Médigue, C and Glaser, P and Pelicic, V},
doi = {10.1186/gb-2009-10-10-r110},
journal = {Genome Biol},
title = {NeMeSys: a biological resource for narrowing the gap between sequence and function in the human pathogen Neisseria meningitidis.},
url = {http://dx.doi.org/10.1186/gb-2009-10-10-r110},
volume = {10},
year = {2009}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - BACKGROUND: Genome sequences, now available for most pathogens, hold promise for the rational design of new therapies. However, biological resources for genome-scale identification of gene function (notably genes involved in pathogenesis) and/or genes essential for cell viability, which are necessary to achieve this goal, are often sorely lacking. This holds true for Neisseria meningitidis, one of the most feared human bacterial pathogens that causes meningitis and septicemia. RESULTS: By determining and manually annotating the complete genome sequence of a serogroup C clinical isolate of N. meningitidis (strain 8013) and assembling a library of defined mutants in up to 60% of its non-essential genes, we have created NeMeSys, a biological resource for Neisseria meningitidis systematic functional analysis. To further enhance the versatility of this toolbox, we have manually (re)annotated eight publicly available Neisseria genome sequences and stored all these data in a publicly accessible online database. The potential of NeMeSys for narrowing the gap between sequence and function is illustrated in several ways, notably by performing a functional genomics analysis of the biogenesis of type IV pili, one of the most widespread virulence factors in bacteria, and by identifying through comparative genomics a complete biochemical pathway (for sulfur metabolism) that may potentially be important for nasopharyngeal colonization. CONCLUSIONS: By improving our capacity to understand gene function in an important human pathogen, NeMeSys is expected to contribute to the ongoing efforts aimed at understanding a prokaryotic cell comprehensively and eventually to the design of new therapies.
AU - Rusniok,C
AU - Vallenet,D
AU - Floquet,S
AU - Ewles,H
AU - Mouzé-Soulama,C
AU - Brown,D
AU - Lajus,A
AU - Buchrieser,C
AU - Médigue,C
AU - Glaser,P
AU - Pelicic,V
DO - 10.1186/gb-2009-10-10-r110
PY - 2009///
TI - NeMeSys: a biological resource for narrowing the gap between sequence and function in the human pathogen Neisseria meningitidis.
T2 - Genome Biol
UR - http://dx.doi.org/10.1186/gb-2009-10-10-r110
UR - https://www.ncbi.nlm.nih.gov/pubmed/19818133
UR - http://hdl.handle.net/10044/1/19663
VL - 10
ER -