Imperial College London

DrVladimirPelicic

Faculty of MedicineDepartment of Medicine

Reader in Molecular Bacteriology & Infection
 
 
 
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Contact

 

+44 (0)20 7594 2080v.pelicic Website

 
 
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Location

 

3.20Flowers buildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Szeto:2011:10.1128/IAI.05313-11,
author = {Szeto, TH and Dessen, A and Pelicic, V},
doi = {10.1128/IAI.05313-11},
journal = {Infect Immun},
pages = {3028--3035},
title = {Structure/function analysis of Neisseria meningitidis PilW, a conserved protein that plays multiple roles in type IV pilus biology.},
url = {http://dx.doi.org/10.1128/IAI.05313-11},
volume = {79},
year = {2011}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Type IV pili (Tfp) are widespread filamentous bacterial organelles that mediate multiple functions and play a key role in pathogenesis in several important human pathogens, including Neisseria meningitidis. Tfp biology remains poorly understood at a molecular level because the roles of the numerous proteins that are involved remain mostly obscure. Guided by the high-resolution crystal structure we recently reported for N. meningitidis PilW, a widely conserved protein essential for Tfp biogenesis, we have performed a structure/function analysis by targeting a series of key residues through site-directed mutagenesis and analyzing the corresponding variants using an array of phenotypic assays. Here we show that PilW's involvement in the functionality of Tfp can be genetically uncoupled from its concurrent role in the assembly/stabilization of the secretin channels through which Tfp emerge on the bacterial surface. These findings suggest that PilW is a multifunctional protein.
AU - Szeto,TH
AU - Dessen,A
AU - Pelicic,V
DO - 10.1128/IAI.05313-11
EP - 3035
PY - 2011///
SP - 3028
TI - Structure/function analysis of Neisseria meningitidis PilW, a conserved protein that plays multiple roles in type IV pilus biology.
T2 - Infect Immun
UR - http://dx.doi.org/10.1128/IAI.05313-11
UR - https://www.ncbi.nlm.nih.gov/pubmed/21646452
VL - 79
ER -