Imperial College London


Faculty of MedicineDepartment of Medicine

Reader in Molecular Bacteriology & Infection



+44 (0)20 7594 2080v.pelicic Website




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BibTex format

author = {Goosens, VJ and Busch, A and Georgiadou, M and Castagnini, M and Forest, KT and Waksman, G and Pelicic, V},
doi = {10.1073/pnas.1618539114},
journal = {Proc Natl Acad Sci U S A},
pages = {E4978--E4986},
title = {Reconstitution of a minimal machinery capable of assembling periplasmic type IV pili.},
url = {},
volume = {114},
year = {2017}

RIS format (EndNote, RefMan)

AB - Type IV pili (Tfp), which are key virulence factors in many bacterial pathogens, define a large group of multipurpose filamentous nanomachines widespread in Bacteria and Archaea. Tfp biogenesis is a complex multistep process, which relies on macromolecular assemblies composed of 15 conserved proteins in model gram-negative species. To improve our limited understanding of the molecular mechanisms of filament assembly, we have used a synthetic biology approach to reconstitute, in a nonnative heterologous host, a minimal machinery capable of building Tfp. Here we show that eight synthetic genes are sufficient to promote filament assembly and that the corresponding proteins form a macromolecular complex at the cytoplasmic membrane, which we have purified and characterized biochemically. Our results contribute to a better mechanistic understanding of the assembly of remarkable dynamic filaments nearly ubiquitous in prokaryotes.
AU - Goosens,VJ
AU - Busch,A
AU - Georgiadou,M
AU - Castagnini,M
AU - Forest,KT
AU - Waksman,G
AU - Pelicic,V
DO - 10.1073/pnas.1618539114
EP - 4986
PY - 2017///
SP - 4978
TI - Reconstitution of a minimal machinery capable of assembling periplasmic type IV pili.
T2 - Proc Natl Acad Sci U S A
UR -
UR -
UR -
VL - 114
ER -