Imperial College London
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DrVahidShahrezaei

Faculty of Natural Sciences, Department of Mathematics

Reader in Biomathematics
 
 
 
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Contact

 

+44 (0)20 7594 8516v.shahrezaei Website

 
 
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Location

 

301BSir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Delaney:2013:10.1101/pdb.top079491,
author = {Delaney, KR and Shahrezaei, V},
doi = {10.1101/pdb.top079491},
journal = {Cold Spring Harb Protoc},
pages = {1115--1124},
title = {Uncaging calcium in neurons.},
url = {http://dx.doi.org/10.1101/pdb.top079491},
volume = {2013},
year = {2013}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Changes in intracellular free calcium concentration (Δ[Ca(2+)]i) driving physiological events such as neurotransmitter release or Ca(2+)-dependent currents can be monitored using Ca(2+)-sensitive fluorescent dyes. Although these dyes can correlate Δ[Ca(2+)]i with a physiological event, they cannot directly test for causality between changes in [Ca(2+)]i and that event. Photolabile Ca(2+) chelators are Ca(2+)-binding molecules that can alter and, to a certain extent, control [Ca(2+)]i in an inducible manner and with temporal and spatial resolution that surpasses microinjection or ionophore application. Here we discuss the properties of caged Ca(2+) compounds as well as some practical considerations for their use in neuronal cells, where they have proven particularly effective.
AU  - Delaney,KR
AU  - Shahrezaei,V
DO  - 10.1101/pdb.top079491
EP  - 1124
PY  - 2013///
SP  - 1115
TI  - Uncaging calcium in neurons.
T2  - Cold Spring Harb Protoc
UR  - http://dx.doi.org/10.1101/pdb.top079491
UR  - https://www.ncbi.nlm.nih.gov/pubmed/24298037
VL  - 2013
ER  -
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