Imperial College London

DrVassoTerzidou

Faculty of MedicineDepartment of Metabolism, Digestion and Reproduction

Clinical Senior Lecturer
 
 
 
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Contact

 

+44 (0)20 7594 2164v.terzidou

 
 
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Location

 

Institute of Reproductive and Developmental BiologyHammersmith Campus

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Summary

 

Publications

Publication Type
Year
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119 results found

Cook J, MacIntyre D, Kim SH, Jones B, Bennett P, Terzidou Vet al., 2016, Hsa-miR-146b-3p is functionally modulated by nuclear factor-kappa B in human myometrial cells, British Maternal & Fetal Medicine Society (BMFMS) 18th Annual Conference 2016, Publisher: WILEY-BLACKWELL, Pages: 102-102, ISSN: 1470-0328

Conference paper

Kim SH, Pohl O, Chollet A, Fairhurst A, Bennett P, Terzidou Vet al., 2016, The inhibition of oxytocin-driven pro-inflammatory effects in both human myometrium and amnion by the oxytocin receptor antagonist, OBE001, British Maternal & Fetal Medicine Society (BMFMS) 18th Annual Conference 2016, Publisher: Wiley, Pages: 11-12, ISSN: 1471-0528

Conference paper

Arulkumaran S, Kim SH, Pohl O, Chollet A, Bennett P, Terzidou Vet al., 2016, The inhibition of both spontaneous and oxytocin-induced contractions of human pregnant myometrium by the oxytocin receptor antagonist, OBE001, British Maternal & Fetal Medicine Society (BMFMS) 18th Annual Conference 2016, Publisher: Wiley, Pages: 103-104, ISSN: 1471-0528

Conference paper

Kindinger L, MacIntyre D, Lee Y, Marchesi J, Smith A, Terzidou V, Lees C, Teoh TG, Bennett Pet al., 2016, Cervical cerclage using braided suture induces vaginal dysbiosis, inflammation, and is associated with increased preterm birth, British Maternal & Fetal Medicine Society (BMFMS) 18th Annual Conference 2016, Publisher: Wiley, Pages: 8-8, ISSN: 1470-0328

Conference paper

Cook J, MacIntyre D, Sykes L, Bennett P, Terzidou Vet al., 2016, Expression of specific cell-free plasma microRNAs is associated with cervical shortening in women at risk of preterm birth, British Maternal & Fetal Medicine Society (BMFMS) 18th Annual Conference 2016, Publisher: WILEY-BLACKWELL, Pages: 103-103, ISSN: 1470-0328

Conference paper

Kim SH, Pohl O, Chollet A, Fairhurst A, Bennett PR, Terzidou Vet al., 2016, The OTR Antagonist, OBE001, Inhibits OT-Driven Proinflammatory Effects in Both Human Myometrium and Amnion, 63rd Annual Scientific Meeting of the Society-for-Reproductive-Investigation, Publisher: SAGE PUBLICATIONS INC, Pages: 123A-123A, ISSN: 1933-7191

Conference paper

Cook JR, MacIntyre DA, Sykes L, Terzidou V, Bennett PRet al., 2016, Prediction of Cervical Shortening and Preterm Delivery Using Specific Cell Free Plasma MicroRNAs, 63rd Annual Scientific Meeting of the Society for Reproductive Investigation, Publisher: SAGE Publications, Pages: 191A-192A, ISSN: 1933-7205

Conference paper

Arulkumaran S, Kim SH, Pohl O, Chollet A, Bennett PR, Terzidou Vet al., 2016, The OTR Antagonist, OBE001, Inhibits Both Spontaneous and OT-Induced Contractions of Human Pregnant Myometrium In Vitro., 63rd Annual Scientific Meeting of the Society-for-Reproductive-Investigation, Publisher: SAGE PUBLICATIONS INC, Pages: 299A-299A, ISSN: 1933-7191

Conference paper

Kim SH, Robinson S, Cook J, Pohl O, Chollet A, Bennett PR, Terzidou Vet al., 2016, The OTR Antagonist, OBE001, Suppresses the Effects of OT on miRNA Expression in Human Myometrium., 63rd Annual Scientific Meeting of the Society-for-Reproductive-Investigation, Publisher: SAGE PUBLICATIONS INC, Pages: 205A-205A, ISSN: 1933-7191

Conference paper

Nawathe AR, Christian M, Kim SH, Johnson M, Savvidou MD, Terzidou Vet al., 2016, Insulin-like growth factor axis in pregnancies affected by fetal growth disorders, Clinical Epigenetics, Vol: 8, ISSN: 1868-7083

Journal article

Kim SH, MacIntyre DA, Hanyaloglu AC, Blanks AM, Thornton S, Bennett PR, Terzidou Vet al., 2015, The oxytocin receptor antagonist, Atosiban, activates pro-inflammatory pathways in human amnion via G(alpha i) signalling, Molecular and Cellular Endocrinology, Vol: 420, Pages: 11-23, ISSN: 1872-8057

Journal article

Pirianov G, MacIntyre DA, Lee Y, Waddington SN, Terzidou V, Mehmet H, Bennett PRet al., 2015, Specific inhibition of c-Jun N-terminal kinase delays preterm labour and reduces mortality, Reproduction, Vol: 150, Pages: 269-277, ISSN: 1741-7899

Preterm labour (PTL) is commonly associated with infection and/or inflammation. Lipopolysaccharide (LPS) from different bacteria can be used to independently or mutually activate Jun N-terminal kinase (JNK)/AP1- or NF-κB-driven inflammatory pathways that lead to PTL. Previous studies using Salmonella abortus LPS, which activates both JNK/AP-1 and NF-κB, showed that selective inhibition of NF-κB delays labour and improves pup outcome. Where labour is induced using Escherichia coli LPS (O111), which upregulates JNK/AP-1 but not NF-κB, inhibition of JNK/AP-1 activation also delays labour. In this study, to determine the potential role of JNK as a therapeutic target in PTL, we investigated the specific contribution of JNK signalling to S. Abortus LPS-induced PTL in mice. Intrauterine administration of S. Abortus LPS to pregnant mice resulted in the activation of JNK in the maternal uterus and fetal brain, upregulation of pro-inflammatory proteins COX-2, CXCL1, and CCL2, phosphorylation of cPLA2 in myometrium, and induction of PTL. Specific inhibition of JNK by co-administration of specific D-JNK inhibitory peptide (D-JNKI) delayed LPS-induced preterm delivery and reduced fetal mortality. This is associated with inhibition of myometrial cPLA2 phosphorylation and proinflammatory proteins synthesis. In addition, we report that D-JNKI inhibits the activation of JNK/JNK3 and caspase-3, which are important mediators of neural cell death in the neonatal brain. Our data demonstrate that specific inhibition of TLR4-activated JNK signalling pathways has potential as a therapeutic approach in the management of infection/inflammation-associated PTL and prevention of the associated detrimental effects to the neonatal brain.

Journal article

Cook JR, Macintyre DA, Samara E, Kim SH, Singh N, Johnson MR, Bennett PR, Terzidou Vet al., 2015, Exogenous oxytocin modulates human myometrial microRNAs, Publisher: WILEY-BLACKWELL, Pages: 226-226, ISSN: 1470-0328

Conference paper

Kim SH, Tulsyan S, Da Silva FMA, Blanks A, Thornton S, Bennett PR, Terzidou Vet al., 2015, The proinflammatory effects of oxytocin in human myometrium require both Gaq and Gai proteins and are not inhibited by the oxytocin receptor antagonist- atosiban, Publisher: WILEY-BLACKWELL, Pages: 356-356, ISSN: 1470-0328

Conference paper

Nawathe A, Savvidou M, Christian M, Johnson M, Terzidou Vet al., 2015, Role of metformin and insulin in epigenetic regulation of IGF axis in women with gestational diabetes, BJOG-AN INTERNATIONAL JOURNAL OF OBSTETRICS AND GYNAECOLOGY, Vol: 122, Pages: 308-308, ISSN: 1470-0328

Journal article

Nawathe A, Savvidou M, Christian M, Johnson M, Terzidou Vet al., 2015, Differences in placental epigenetic regulation in pregnancies affected by fetal growth disorders, BJOG-AN INTERNATIONAL JOURNAL OF OBSTETRICS AND GYNAECOLOGY, Vol: 122, Pages: 62-62, ISSN: 1470-0328

Journal article

Yulia A, Thomas S, Singh N, Johnson MR, Wales NM, Terzidou Vet al., 2015, Pregnancy outcome following the indication of cerclage, BJOG-AN INTERNATIONAL JOURNAL OF OBSTETRICS AND GYNAECOLOGY, Vol: 122, Pages: 226-227, ISSN: 1470-0328

Journal article

Cook J, MacIntyre D, Samara E, Kim SH, Singh N, Johnson M, Bennett P, Terzidou Vet al., 2015, Exogenous oxytocin modulates human myometrial microRNAs, Publisher: Elsevier, Pages: E1-E9, ISSN: 1097-6868

Conference paper

Cook JR, MacIntyre DA, Samara E, Kim S, Singh NKP, Johnson MRD, Bennett PR, Terzidou Vet al., 2015, Exogenous oxytocin modulates human myometrial microRNAs, American Journal of Obstetrics and Gynecology, Vol: 213, Pages: 65.e1-65.e9, ISSN: 0002-9378

Objective: MicroRNAs (miRNAs) play a modulatory role in pathways that lead to labor onset, although oxytocin is known to modulate gene expression within the myometrium. We aimed to identify miRNAs whose expression is regulated by oxytocin in pregnant human myometrium. Study Design: Myometrial miRNA expression profiles were compared between samples collected from women at term before the onset of labor (no labor; n= 8) and after labor onset after early exogenous oxytocin treatment (n= 8). Multivariate modelling was used to assess differences in miRNA profiles. Biologic validation was undertaken on 3 independent patient cohorts (no labor, n= 10; labor induced with oxytocin, n= 8; and spontaneous labor with no oxytocin treatment, n= 10). Invitro studies that used primary myocytes were undertaken to assess target miRNA expression after oxytocin treatment. Target genes of candidate miRNAs were identified in silico and cross-referenced with genes that are known to be associated with labor or expressed in myometrium. Results: In total, 1309 miRNAs were analyzed by microarray, of which 494 were detected in human myometrium. Multivariate modeling identified 12 target miRNAs the differential expression of which was most responsible for the observed separation of the 2 patient populations in the primary discovery cohorts. Biologic validation in the independent secondary sample cohorts showed that oxytocin independently regulated 5 miRNAs (hsa-miR-146b-3p, hsa-miR-196b-3p, hsa-miR-223-3p, hsa-miR-873-5p, and hsa-miR-876-5p). Additionally, hsa-miR-146b-3p was increased both in labor that was induced with oxytocin and in myometrium from spontaneous labor with no oxytocin treatment compared with no labor samples. Four of the validated miRNAs (hsa-miR-146a-5p, hsa-miR-146b-3p, hsa-miR-196b-3p, and hsa-miR-876-5p) were expressed in primary human myocytes; oxytocin treatment of these cells replicated the directional changes that were observed invivo. Conclusion: Oxytocin alters the e

Journal article

Kim SH, MacIntyre DA, Da Silva MF, Blanks AM, Lee YS, Thornton S, Bennett PR, Terzidou Vet al., 2015, Oxytocin activates NF-kappa B-mediated inflammatory pathways in human gestational tissues, MOLECULAR AND CELLULAR ENDOCRINOLOGY, Vol: 403, Pages: 64-77, ISSN: 0303-7207

Journal article

Cook JR, MacIntyre DA, Samara E, Kim SH, Singh N, Johnson MR, Bennett PR, Terzidou Vet al., 2015, Oxytocin Modulates a Unique Set of Human Myometrial MicroRNAs, Publisher: SAGE PUBLICATIONS INC, Pages: 67A-67A, ISSN: 1933-7191

Conference paper

Nawathe A, Hye KS, Savvidou M, Christian M, Terzidou Vet al., 2015, Gestational Diabetes and Epigenetic Dysfunction of the IGF Axis and Its Binding Proteins., REPRODUCTIVE SCIENCES, Vol: 22, Pages: 254A-254A, ISSN: 1933-7191

Journal article

Cook JR, MacIntyre DA, Samara E, Singh N, Bennett PR, Terzidou Vet al., 2014, hsa-miR-223 is differentially expressed in labouring and non-labouring human myometrium at term, Publisher: WILEY-BLACKWELL, Pages: E4-E4, ISSN: 1470-0328

Conference paper

Nawathe A, Hye KS, Savvidou M, Christian M, Johnson M, Terzidou Vet al., 2014, Insulin-like growth factors and binding protein expressions in fetal growth disorders and maternal diabetes, BJOG-AN INTERNATIONAL JOURNAL OF OBSTETRICS AND GYNAECOLOGY, Vol: 121, Pages: E2-E3, ISSN: 1470-0328

Journal article

Singh N, Yuiia A, Terzidou V, Sooranna S, Johnson Met al., 2014, 6.3 The Commonalities and Differences in the Molecular Expression of Different Phenotype-specific Causes of Preterm Labour in Comparison to Term Labour., Arch Dis Child Fetal Neonatal Ed, Vol: 99

: Preterm birth comprises of several distinct clinical phenotypes. The commonalities and differences of the genes altered in the different phenotypes of preterm labour (PTL) have yet to be elucidated. Myometrial biopsies were collected during caesarean section from women in preterm no labour (PNL; n = 26), preterm labour (chorioamnionitis, placental abruption, polyhydramnios and idiopathic; n = 11, 6, 16 and 4 respectively), preterm twins (NL and L; n = 12), term no labour (n = 18), term early labour (EAL, <3 cm dilatation; n = 10) and established labour (ESL, ≥3 cm dilatation; n = 12). Samples were rapidly frozen at -80(0)C. Total RNA was extracted using RNeasy kit from Qiagen and converted to cDNA. Copy numbers of PGHS-2, CXCL-8, oxytocin receptor, IL-6, connexin 43 and GAPDH were measured by qPCR using Rotor-Gene(TM) (Corbett Research, Australia). In PTL compared to PNL, PGHS-2 was significantly overall increased in all the phenotype specific groups except placental abruption (p < 0.05). PGHS-2 levels were also significantly increased in twin no labour versus PNL (p < 0.05), but there was no difference in PGHS-2 expression between twin no labour and twin labour. There was no statistical difference in the expression of PGHS-2 when the different phenotypes of PTL were compared to both EAL and ESL. There was no significant change in the expression of CXCL-8 or OTR among both the labouring and non-labouring preterm phenotypes. Connexin-43 expression did not change among the preterm groups. These data show that PGHS-2 appears to play a significant role in driving the process of preterm labour in all distinct phenotypes (except placental abruption).

Journal article

Kim SH, Blanks A, Thornton S, Bennett P, Terzidou Vet al., 2014, 6.2 Atosiban activates NF-κB and pro-inflammatory pathways in human amnion via Gαi signalling., Pages: A7-A8

: Inflammation is recognized as one of the key characteristics of both preterm and term labour. There is accumulating evidence suggesting that NF-κB plays a significant role in the physiology of human labour. NF-κB has been shown to increase in human amnion in association with labour. In term pre-labour amniocytes, OT couples with Gαi, but not Gαq, to induce sequential activation of MAPKs and NF-κB to increase expression of downstream pro-labour genes including PG synthetic enzymes and inflammatory cytokines/chemokines. We have previously reported that the OTR antagonist, atosiban, does not inhibit, but stimulates both MAPKs and NF-κB in amnion. Here, we investigate the downstream effects of NF-κB activation by atosiban and the relevant G protein coupling involved. Following activation of MAPKs and NF-κB with atosiban stimulation, there were significant increases in mRNA expressions of NF-κB-regulated genes; IL-6, CCL5, and COX-2, and increases in the release of IL-6 and CCL5 after 2 h and 6 h, respectively (p < 0.05, ANOVA). In addition, upregulation of COX-2 and activation of cPLA2 were observed at protein level, as well as the subsequent PGE2 production (p < 0.05, ANOVA). Pretreatment with PTX reduced the effect of atosiban on NF-κB, ERK and p38 activation, and inhibited COX-2 and p-cPLA2 expression, indicating that these effects are mediated through Gαi (p < 0.05, ANOVA). We conclude that atosiban induces activation of NF-κB and increase expression of downstream pro-labour genes via OTR- Gαi coupling. Therefore, therapeutic modulation of the OT/OTR system for clinical management of term/preterm labour should consider potential inflammatory activation by ligand-directed signalling.

Conference paper

Kay O, Hughes A, Saade G, Bennett P, Terzidou V, Thornton Set al., 2014, Recent advances in the prevention and treatment of preterm labour: Oxytocin antagonists and the silicone (Arabin) pessary, Fetal and Maternal Medicine Review, Vol: 25, Pages: 134-145, ISSN: 0965-5395

© 2015 Cambridge University Press. Preterm birth may be spontaneous or medically indicated for maternal or fetal reasons. Around 20-25% of preterm births (PTB) follow preterm premature rupture of the membranes (PPROM), however the cause of preterm labour is often unknown. It may represent early maturation and activation of the normal labour process or it may be precipitated by pathological causes. The normal process of labour has a diurnal variation with more deliveries occurring at night. Evidence demonstrating that the diurnal variation persists in preterm deliveries suggest that at least a proportion are due to early maturation of the normal process and the logical assumption is that these may be amenable to prevention or effective treatment. Whatever the cause of preterm delivery, there appears to be a common pathway resulting in activation of inflammatory processes. It is important to distinguish the physiological and pathological causes of preterm labour and not to assume that all inflammation is pathological. The distinction is clinically important since pathological causes may be associated with an adverse intrauterine environment, which would be a contraindication to delaying delivery.

Journal article

Cook JR, Chatfield S, Chandiramani M, Sykes L, Simcox R, Abbott D, Jones B, Loudon J, Shennan A, Terzidou V, Bennett PRet al., 2014, Cerclage Position Predicts Risk of Preterm Delivery in Women Undergoing Ultrasound-Indicated Cervical Cerclage., REPRODUCTIVE SCIENCES, Vol: 21, Pages: 138A-138A, ISSN: 1933-7191

Journal article

Yulia A, Singh N, Lei K, Sooranna SR, Terzidou V, Johnson MRet al., 2014, Molecular Markers of cAMP Related Genes during Gestation and Parturition in Human Myometrium, REPRODUCTIVE SCIENCES, Vol: 21, Pages: 347A-347A, ISSN: 1933-7191

Journal article

Yulia A, Georgiou EX, Das A, Lei K, Sooranna SR, Terzidou V, Johnson MRet al., 2014, Enhancement of Anti-Inflammatory Effect by the Combination of Progesterone and cAMP on IL-1b-Stimulated Human Myometrial Cells., REPRODUCTIVE SCIENCES, Vol: 21, Pages: 111A-111A, ISSN: 1933-7191

Journal article

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