Imperial College London

DrVassoTerzidou

Faculty of MedicineDepartment of Metabolism, Digestion and Reproduction

Clinical Senior Lecturer
 
 
 
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Contact

 

+44 (0)20 7594 2164v.terzidou

 
 
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Location

 

Institute of Reproductive and Developmental BiologyHammersmith Campus

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Summary

 

Publications

Publication Type
Year
to

119 results found

Cook JR, MacIntyre DA, Samara E, Singh N, Bennett PR, Terzidou Vet al., 2014, Identification of Differentially Expressed MicroRNAs in Labouring and Non-Labouring Human Myometrium at Term., 61st Annual Scientific Meeting of the Society-for-Gynecologic-Investigation (SGI), Publisher: SAGE PUBLICATIONS INC, Pages: 138A-138A, ISSN: 1933-7191

Conference paper

Ibeto L, Chandiramani M, Cook JR, Chatfield S, Sykes L, Shennan AH, Bennett PR, Terzidou Vet al., 2014, The Risk of Preterm Birth Associated with Uterine Anomalies and the Clinical Utility of Serial Measurement of Cervical Length, REPRODUCTIVE SCIENCES, Vol: 21, Pages: 253A-253A, ISSN: 1933-7191

Journal article

Kim SH, Blanks A, Thornton S, Bennett PR, Terzidou Vet al., 2014, Oxytocin Receptor Antagonist, Atosiban, Drives Proinflammatory Effects in Human Amnion Via G(ai) Signaling., 61st Annual Scientific Meeting of the Society-for-Gynecologic-Investigation (SGI), Publisher: SAGE PUBLICATIONS INC, Pages: 111A-111A, ISSN: 1933-7191

Conference paper

Da Silva MAF, Kim SH, Bennett PR, Terzidou Vet al., 2014, Oxytocin Activates NF-kB in Human Myometrium, 61st Annual Scientific Meeting of the Society-for-Gynecologic-Investigation (SGI), Publisher: SAGE PUBLICATIONS INC, Pages: 241A-241A, ISSN: 1933-7191

Conference paper

Nawathe A, Hye KS, Savvidou M, Christian M, Johnson M, Terzidou Vet al., 2014, Placental Expression of IGFs and Their Binding Proteins in Pregnancies Affected by Growth Disorders, Diabetes and Obesity., REPRODUCTIVE SCIENCES, Vol: 21, Pages: 294A-294A, ISSN: 1933-7191

Journal article

Nawathe A, Hye KS, Savvidou M, Christian M, Johnson M, Terzidou Vet al., 2014, Effect of Adiponectin on IL-6 and COX-2 Expression in Term Placental Explants, REPRODUCTIVE SCIENCES, Vol: 21, Pages: 370A-370A, ISSN: 1933-7191

Journal article

Cook JR, MacIntyre DA, Bennett P, Terzidou Vet al., 2013, Specific microRNAs are differentially expressed in labouring and non-labouring human myometrium at term, BJOG-AN INTERNATIONAL JOURNAL OF OBSTETRICS AND GYNAECOLOGY, Vol: 120, Pages: 13-14, ISSN: 1470-0328

Journal article

MacIntyre DA, Migale R, Lee YS, Herbert B, Sykes L, Terzidou V, Johnson MR, Bennett PRet al., 2013, TLR4-Mediated Activation of AP1 Via JNK Induces Preterm Labour in the Mouse., 60th Annual Scientific Meeting of the Society-for-Gynecologic-Investigation (SGI), Publisher: SAGE PUBLICATIONS INC, Pages: 296A-296A, ISSN: 1933-7191

Conference paper

Kim SH, Blanks A, Thornton S, Johnson M, Bennett PR, Terzidou Vet al., 2013, Activation of NF-kappa B in Human Amnion Is Mediated through G(ai) and Is Mimicked by the Oxytocin Receptor Antagonist - Atosiban., 60th Annual Scientific Meeting of the Society-for-Gynecologic-Investigation (SGI), Publisher: SAGE PUBLICATIONS INC, Pages: 122A-122A, ISSN: 1933-7191

Conference paper

Lee Y, Sooranna SR, Terzidou V, Christian M, Brosens J, Huhtinen K, Poutanen M, Barton G, Johnson MR, Bennett PRet al., 2012, Interactions between inflammatory signals and the progesterone receptor in regulating gene expression in pregnant human uterine myocytes, JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, Vol: 16, Pages: 2487-2503, ISSN: 1582-1838

The absence of a fall in circulating progesterone levels has led to the concept that human labour is associated with ‘functional progesterone withdrawal’ caused through changes in the expression or function of progesterone receptor (PR). At the time of labour, the human uterus is heavily infiltrated with inflammatory cells, which release cytokines to create a ‘myometrial inflammation’ via NF‐κB activation. The negative interaction between NF‐κB and PR, may represent a mechanism to account for ‘functional progesterone withdrawal’ at term. Conversely, PR may act to inhibit NF‐κB function and so play a role in inhibition of myometrial inflammation during pregnancy. To model this inter‐relationship, we have used small interfering (si) RNA‐mediated knock‐down of PR in human pregnant myocytes and whole genome microarray analysis to identify genes regulated through PR. We then activated myometrial inflammation using IL‐1β stimulation to determine the role of PR in myometrial inflammation regulation. Through PR‐knock‐down, we found that PR regulates gene networks involved in myometrial quiescence and extracellular matrix integrity. Activation of myometrial inflammation was found to antagonize PR‐induced gene expression, of genes normally upregulated via PR. We found that PR does not play a role in repression of pro‐inflammatory gene networks induced by IL‐1β and that only MMP10 was significantly regulated in opposite directions by IL‐1β and PR. We conclude that progesterone acting through PR does not generally inhibit myometrial inflammation. Activation of myometrial inflammation does cause ‘functional progesterone withdrawal’ but only in the context of genes normally upregulated via PR.

Journal article

Lim S, MacIntyre DA, Lee YS, Khanjani S, Terzidou V, Teoh TG, Bennett PRet al., 2012, Nuclear Factor Kappa B Activation Occurs in the Amnion Prior to Labour Onset and Modulates the Expression of Numerous Labour Associated Genes, PLOS ONE, Vol: 7, ISSN: 1932-6203

Journal article

Khanjani S, Terzidou V, Johnson MR, Bennett PRet al., 2012, NF kappa B and AP-1 Drive Human Myometrial IL8 Expression, MEDIATORS OF INFLAMMATION, Vol: 2012, ISSN: 0962-9351

The uterine expression of the chemokine IL8 increases dramatically with the onset of labour both at term and preterm. The IL8 promoter contains binding sites for the transcription factors nuclear factor-kappa B (NFκB), activator protein-1 (AP-1), and CCAAT/enhancer-binding protein (CEBP). In this study we investigated the roles of these transcription factors in IL1B regulation of the IL8 gene in human myometrium. Using chromatin immune precipitation (ChIP) assay, we showed that each of NFκB, CEBP, and AP-1 binds to the IL8 promoter upon IL1B stimulation. To examine the relative importance of each site in IL8 gene expression, site-directed mutagenesis of each of these sites was performed. We found that the NFκB site was essential for basal and IL1B-stimulated gene expression. Mutation of the AP-1 site reduced both basal and IL1B-stimulated expression but to a lesser extent. Mutation of the CEBP site had no effect upon basal expression but eliminated the IL1B response. Small interfering RNA (siRNA) silencing of NFκB abolished the IL8 response to IL1B significantly; siRNA against AP-1 reduced it to a lesser extent whilst knockdown of CEBP enhanced the response. Our data confirms a central and essential role for NFκB in regulation of IL8 in human myometrium.

Journal article

Webster SNE, Wales NM, Terzidou V, Steer PJet al., 2012, Pregnancy Outcome Following History Indicated Abdominal Cerclage, REPRODUCTIVE SCIENCES, Vol: 19, Pages: 373A-374A, ISSN: 1933-7191

Journal article

Tchipeva D, Semertzidou A, Read J, Takahashi J, Kim SH, Johnson M, Bennett PR, Terzidou Vet al., 2012, Suppression of the SUMO Pathway in Human Myometrium Modulates PR and NF-kB Signalling., Publisher: SAGE PUBLICATIONS INC, Pages: 187A-187A, ISSN: 1933-7191

Conference paper

Read JE, Kim SH, Tchipeva D, Williamson C, Bennett PR, Terzidou Vet al., 2012, Bile Acids Upregulate the Oxytocin Receptor and Increase Contractility in Human Myometrium, Publisher: SAGE PUBLICATIONS INC, Pages: 198A-198A, ISSN: 1933-7191

Conference paper

Kim SH, Blanks A, Thornton S, Johnson M, Bennett PR, Terzidou Vet al., 2012, Oxytocin Mediates COX-2 Expression Via NF-kappa B and MAPK Pathway., Publisher: SAGE PUBLICATIONS INC, Pages: 184A-184A, ISSN: 1933-7191

Conference paper

Lee YS, MacIntyre DA, Pirianov G, Herbert B, Terzidou V, Johnson MR, Bennett PRet al., 2012, Inhibition of AP1 Delays LPS-Induced Preterm Labour and Improves Pup Outcome in the Murine Model, Publisher: SAGE PUBLICATIONS INC, Pages: 120A-120A, ISSN: 1933-7191

Conference paper

Takahashi J, Kim SH, Sermertzidou A, Read J, Tchipeva D, MacIntyre DA, Brosens J, Bennett P, Terzidou Vet al., 2012, Regulation of Sumo-Pathway Enzymes upon Inflammatory Stress and Labour, 59th Annual Scientific Meeting of the Society-for-Gynecologic-Investigation, Pages: 187A-187A

Conference paper

Khanjani S, Terzidou V, Lee YS, Thornton S, Johnson MR, Bennett PRet al., 2011, Synergistic Regulation of Human Oxytocin Receptor Promoter by CCAAT/Enhancer-Binding Protein and RELA, BIOLOGY OF REPRODUCTION, Vol: 85, Pages: 1083-1088, ISSN: 0006-3363

Journal article

Kim SH, Blanks A, Thornton S, Johnson M, Bennett PR, Terzidou Vet al., 2011, Cytokines Regulated by Oxytocin in Human Amnion, Pages: 299A-299A, ISSN: 1933-7191

Conference paper

Terzidou V, Blanks AM, Kim SH, Thornton S, Bennett PRet al., 2011, Labor and Inflammation Increase the Expression of Oxytocin Receptor in Human Amnion, BIOL REPROD, Vol: 84, Pages: 546-552, ISSN: 0006-3363

Journal article

Semertzidou A, Brosens J, Bennett PR, Johnson M, Terzidou Vet al., 2011, SUMO Modification Is Linked to the Process of Human Labor by Modulating PR Signalling, REPROD SCI, Vol: 18, Pages: 299A-300A, ISSN: 1933-7191

Journal article

Kim SH, Blanks A, Thornton S, Johnson M, Bennett PR, Terzidou Vet al., 2011, Expression and Regulation of cPLA2 Isoforms in Human Amnion, Pages: 300A-300A, ISSN: 1933-7191

Conference paper

Lee YS, Nelson S, Bollopragada S, Terzidou V, Sooranna S, Norman J, Johnson MR, Bennett PRet al., 2011, Transcriptomic Analysis of Human Myometrium Supports Inflammation but Not PR-Mediated Functional Progesterone Withdrawal as a Mechanism for Changes in Gene Expression with Labour, REPROD SCI, Vol: 18, Pages: 242A-242A, ISSN: 1933-7191

Journal article

Khanjani S, Kandola MK, Lindstrom TM, Sooranna SR, Melchionda M, Lee YS, Terzidou V, Johnson MR, Bennett PRet al., 2010, NF-kB regulates a cassette of immune/inflammatory genes in human pregnant myometrium at term, Journal of Cellular and Molecular Medicine, Vol: 15, Pages: 809-824, ISSN: 1582-4934

The onset of human labour resembles inflammation with increased synthesis of prostaglandins and cytokines. There is evidence from rodent models for an important role for nuclear factor-κB (NF-κB) activity in myometrium which both up-regulates contraction-associated proteins and antagonizes the relaxatory effects of progesterone. Here we show that in the human, although there are no differences in expression of NF-κB p65, or IκB-α between upper- or lower-segment myometrium or before or after labour, there is nuclear localization of serine-256-phospho-p65 and serine-536-phospho-p65 in both upper- and lower-segment myometrium both before and after the onset of labour at term. This shows that NF-κB is active in both upper and lower segment prior to the onset of labour at term. To identify the range of genes regulated by NF-κB we overexpressed p65 in myocytes in culture. This led to NF-κB activation identical to that seen following interleukin (IL)-1β stimulation, including phosphorylation and nuclear translocation of p65 and p50. cDNA microarray analysis showed that NF-κB increased expression of 38 genes principally related to immunity and inflammation. IL-1β stimulation also resulted in an increase in the expression of the same genes. Transfection with siRNA against p65 abolished the response to IL-1β proving a central role for NF-κB. We conclude that NF-κB is active in myocytes in both the upper and lower segment of the uterus prior to the onset of labour at term and principally regulates a group of immune/inflammation associated genes, demonstrating that myocytes can act as immune as well as contractile cells.

Journal article

Terzidou V, Kandola MS, Mandeep PR, 2010, Labour Associated Differences in miRNA Expression in Human Myometrium, 57th Annual Meeting of the Society-for-Gynecologic-Investigation, Publisher: SAGE PUBLICATIONS INC, Pages: 255A-255A, ISSN: 1933-7191

Conference paper

Lee Y, Sooranna S, Terzidou V, Brosens J, Putanen M, Huhtinen K, Johnson M, Bennett Pet al., 2010, Identification of Progesterone Responsive Genes in Human Myometrium, Pages: 156A-156A, ISSN: 1933-7191

Conference paper

Terzidou V, Sooranna SR, Johnson M, Bennett PR, Brosens JJet al., 2010, A Role for the SUMO Pathway in Human Labour., Pages: 71A-71A, ISSN: 1933-7191

Conference paper

Khanjani S, Sooranna SR, Melchionda M, Lee YS, Terzidou V, Johnson MR, Bennett PRet al., 2009, Nuclear Factor Kappa B Is Essential but Not Sufficient for PGHS-2 Expression in Human Myometrium., 56th Annual Meeting of the Society-for-Gynecologic-Investigation, Publisher: SAGE PUBLICATIONS INC, Pages: 75A-75A, ISSN: 1933-7191

Conference paper

Khanjani S, Sooranna SR, Melchionda M, Lee YS, Terzidou V, Johnson MR, Bennett PRet al., 2009, Myocytes as Immune Cells: NF-kappa B Regulates a Group of Immune/Inflammatory Genes, Pages: 117A-117A, ISSN: 1933-7191

Conference paper

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