Imperial College London
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Vincenzo De Paola

Faculty of Medicine, Department of Brain Sciences

Reader in Translational Neuroscience
 
 
 
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Contact

 

+44 (0)20 7594 2501vincenzo.depaola Website CV

 
 
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Assistant

 

Miss Lydia Lawson +44 (0)20 7594 1264

 
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Location

 

Burlington DanesHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Bloomfield:2018:10.1177/0269881118788830,
author = {Bloomfield, PS and Bonsall, D and Wells, L and Dormann, D and Howes, O and De, Paola V},
doi = {10.1177/0269881118788830},
journal = {Journal of Psychopharmacology},
pages = {1264--1272},
title = {The effects of haloperidol on microglial morphology and translocator protein levels: An in vivo study in rats using an automated cell evaluation pipeline},
url = {http://dx.doi.org/10.1177/0269881118788830},
volume = {32},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - BACKGROUND: Altered microglial markers and morphology have been demonstrated in patients with schizophrenia in post-mortem and in vivo studies. However, it is unclear if changes are due to antipsychotic treatment. AIMS: Here we aimed to determine whether antipsychotic medication affects microglia in vivo. METHODS: To investigate this we administered two clinically relevant doses (0.05 mg n=12 and 2.5 mg n=7 slow-release pellets, placebo n=20) of haloperidol, over 2 weeks, to male Sprague Dawley rats to determine the effect on microglial cell density and morphology (area occupied by processes and microglial cell area). We developed an analysis pipeline for the automated assessment of microglial cells and used lipopolysaccharide (LPS) treatment ( n=13) as a positive control for analysis. We also investigated the effects of haloperidol ( n=9) or placebo ( n=10) on the expression of the translocator protein 18 kDa (TSPO) using autoradiography with [3H]PBR28, a TSPO ligand used in human positron emission tomography (PET) studies. RESULTS: Here we demonstrated that haloperidol at either dose does not alter microglial measures compared with placebo control animals ( p > 0.05). Similarly there was no difference in [3H]PBR28 binding between placebo and haloperidol tissue ( p > 0.05). In contrast, LPS was associated with greater cell density ( p = 0.04) and larger cell size ( p = 0.01). CONCLUSION: These findings suggest that haloperidol does not affect microglial cell density, morphology or TSPO expression, indicating that clinical study alterations are likely not the consequence of antipsychotic treatment. The automated cell evaluation pipeline was able to detect changes in microglial morphology induced by LPS and is made freely available for future use.
AU  - Bloomfield,PS
AU  - Bonsall,D
AU  - Wells,L
AU  - Dormann,D
AU  - Howes,O
AU  - De,Paola V
DO  - 10.1177/0269881118788830
EP  - 1272
PY  - 2018///
SN  - 1461-7285
SP  - 1264
TI  - The effects of haloperidol on microglial morphology and translocator protein levels: An in vivo study in rats using an automated cell evaluation pipeline
T2  - Journal of Psychopharmacology
UR  - http://dx.doi.org/10.1177/0269881118788830
UR  - https://www.ncbi.nlm.nih.gov/pubmed/30126329
UR  - http://hdl.handle.net/10044/1/69249
VL  - 32
ER  -
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