Imperial College London
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Vincenzo De Paola

Faculty of Medicine, Department of Brain Sciences

Reader in Translational Neuroscience
 
 
 
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Contact

 

+44 (0)20 7594 2501vincenzo.depaola Website CV

 
 
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Assistant

 

Miss Lydia Lawson +44 (0)20 7594 1264

 
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Location

 

Burlington DanesHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Real:2018:10.1126/science.aau1810,
author = {Real, R and Peter, M and Trabalza, A and Khan, S and Smith, MA and Dopp, J and Barnes, SJ and Momoh, A and Strano, A and Volpi, E and Knott, G and Livesey, FJ and De, Paola V},
doi = {10.1126/science.aau1810},
journal = {Science},
title = {In vivo modeling of human neuron dynamics and Down syndrome.},
url = {http://dx.doi.org/10.1126/science.aau1810},
volume = {362},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Harnessing the potential of human stem cells for modeling the physiology and diseases of cortical circuitry requires monitoring cellular dynamics in vivo. We show that human induced pluripotent stem cell (iPSC)-derived cortical neurons transplanted into the adult mouse cortex consistently organized into large (up to ~100 mm3) vascularized neuron-glia territories with complex cytoarchitecture. Longitudinal imaging of >4000 grafted developing human neurons revealed that neuronal arbors refined via branch-specific retraction; human synaptic networks substantially restructured over 4 months, with balanced rates of synapse formation and elimination; and oscillatory population activity mirrored the patterns of fetal neural networks. Lastly, we found increased synaptic stability and reduced oscillations in transplants from two individuals with Down syndrome, demonstrating the potential of in vivo imaging in human tissue grafts for patient-specific modeling of cortical development, physiology, and pathogenesis.
AU  - Real,R
AU  - Peter,M
AU  - Trabalza,A
AU  - Khan,S
AU  - Smith,MA
AU  - Dopp,J
AU  - Barnes,SJ
AU  - Momoh,A
AU  - Strano,A
AU  - Volpi,E
AU  - Knott,G
AU  - Livesey,FJ
AU  - De,Paola V
DO  - 10.1126/science.aau1810
PY  - 2018///
TI  - In vivo modeling of human neuron dynamics and Down syndrome.
T2  - Science
UR  - http://dx.doi.org/10.1126/science.aau1810
UR  - https://www.ncbi.nlm.nih.gov/pubmed/30309905
UR  - http://hdl.handle.net/10044/1/65663
VL  - 362
ER  -
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