Imperial College London
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Vincenzo De Paola

Faculty of Medicine, Department of Brain Sciences

Reader in Translational Neuroscience
 
 
 
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Contact

 

+44 (0)20 7594 2501vincenzo.depaola Website CV

 
 
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Assistant

 

Miss Lydia Lawson +44 (0)20 7594 1264

 
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Location

 

Burlington DanesHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Jackson:2015:10.1002/0471142301.ns0224s73,
author = {Jackson, J and Canty, AJ and Huang, L and De, Paola V},
doi = {10.1002/0471142301.ns0224s73},
journal = {Curr Protoc Neurosci},
pages = {2.24.1--2.24.17},
title = {Laser-Mediated Microlesions in Mouse Neocortex to Investigate Neuronal Degeneration and Regeneration.},
url = {http://dx.doi.org/10.1002/0471142301.ns0224s73},
volume = {73},
year = {2015}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - In vivo two-photon (2P) imaging enables neural circuitry to be repeatedly visualized in both normal conditions and following trauma. This protocol describes how laser-mediated neuronal microlesions can be created in the cerebral cortex using an ultrafast laser without causing a significant inflammatory reaction or compromising the blood-brain barrier. Furthermore, directives are provided for the acute and chronic in vivo imaging of the lesion site, as well as for post-hoc analysis of the lesion site in fixed tissue, which can be correlated with the live imaging phase.
AU  - Jackson,J
AU  - Canty,AJ
AU  - Huang,L
AU  - De,Paola V
DO  - 10.1002/0471142301.ns0224s73
EP  - 24
PY  - 2015///
SP  - 2
TI  - Laser-Mediated Microlesions in Mouse Neocortex to Investigate Neuronal Degeneration and Regeneration.
T2  - Curr Protoc Neurosci
UR  - http://dx.doi.org/10.1002/0471142301.ns0224s73
UR  - https://www.ncbi.nlm.nih.gov/pubmed/26426385
VL  - 73
ER  -
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