Publications
191 results found
Childs RA, Palma AS, Wharton S, et al., 2010, Receptor-binding specificity of pandemic influenza A (H1N1) 2009 virus determined by carbohydrate microarray (vol 27, pg 797, 2009), NATURE BIOTECHNOLOGY, Vol: 28, Pages: 178-178, ISSN: 1087-0156
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- Citations: 2
Liu Y, Childs RA, Matrosovich T, et al., 2010, Altered receptor specificity and cell tropism of D222G haemagglutinin mutants from fatal cases of pandemic A (H1N1) 2009 influenza, J. Virol., Vol: 84, Pages: 12069-12074
Boyle M, Richards JS, Gilson PR, et al., 2010, Heparin-Like Molecules Inhibit Initial Events in Erythrocyte Invasion by Plasmodium falciparum Merozoites and Bind to Merozoite Surface Protein 1, Blood, Vol: 115, Pages: 4559-4568
Chen S, Wang J, Xue C, et al., 2010, Sulfation of a Squid Ink Polysaccharide and its Inhibitory Effect on Tumor Cell Metastasis, Carbohydr. Polymers, Vol: 81, Pages: 560-566
Palma AS, Liu Y, Muhle-Goll C, et al., 2010, Multifaceted approaches including neoglycolipid oligosaccharide microarrays to ligand discovery for malectin, Methods Enzymol., Vol: 478, Pages: 265-286
In this chapter, we describe the key procedures for isolation of the oligosaccharides and the preparation of neoglycolipid probes together with expression of malectin that have enabled the discovery of the highly selective binding of this newly described protein in the endoplasmic reticulum (ER) to a diglucosyl high-mannose N-glycan. This is the first indication of a bioactivity for a diglucosyl high-mannose N-glycan of the type that occurs in the ER of eukaryotic cells and which is an intermediate in the early steps of the N-glycosylation pathway of nascent proteins. The malectin story is an example of a powerful convergence of disciplines in biological sciences: (i) developmental biology, (ii) bioinformatics, (iii) recombinant protein expression, (iv) protein structural studies, (v) glucan biochemistry, and (vi) drug-assisted engineering of oligosaccharide biosynthesis, culminating in (vii) oligosaccharide "designer" microarrays, to clinch the remarkable selectivity of the binding of this newly discovered ER protein. Thus, the way is open to the identification of the role of malectin in the N-glycosylation pathway
Ewers H, Römer W, Smith AE, et al., 2010, SV40 Binding to Its Receptor, GM1, Induces Membrane Invagination, Tubulation, and Infection, Nature Cell Biol, Vol: 12, Pages: 11-18
Boyle M, Richards JS, Gilson PR, et al., 2009, CELLULAR AND MOLECULAR INTERACTIONS WITH HEPARIN-LIKE MOLECULES DURING INVASION OF ERYTHROCYTES BY <i>PLASMODIUM FALCIPARUM</i> MEROZOITES, 58th Annual Meeting of the American-Society-of-Tropical-Medicine-and-Hygiene, Publisher: AMER SOC TROP MED & HYGIENE, Pages: 90-91, ISSN: 0002-9637
Liu Y, Palma AS, Chai W, et al., 2009, The Latest on Malectin - A Lectin of the Endoplasmic Reticulum, Annual Meeting of the Society-for-Glycobiology, Publisher: OXFORD UNIV PRESS INC, Pages: 1301-1302, ISSN: 0959-6658
Childs RA, Palma AS, Wharton S, et al., 2009, Receptor-binding specificity of pandemic influenza A (H1N1) 2009 virus determined by carbohydrate microarray, Nat.Biotechnol., Vol: 27, Pages: 797-799
Yang B, Yu G, Zhao X, et al., 2009, Mechanism of mild acid hydrolysis of galactan polysaccharides with highly ordered disaccharide repeats leading to a complete series of exclusively odd-numbered oligosaccharides, FEBS JOURNAL, Vol: 276, Pages: 2125-2137, ISSN: 1742-464X
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- Citations: 115
Palma AS, Liu Y, Campanero-Rhodes MA, et al., 2008, Malectin-a Novel Lectin of the Endoplasmic Reticulum and a Candidate New Player in the Early Steps Of Protein N-Glycosylation, Annual Meeting of the Society-for-Glycobiology, Publisher: OXFORD UNIV PRESS INC, Pages: 948-949, ISSN: 0959-6658
Chen S, Xu J, Xue C, et al., 2008, Sequence determination of a non-sulfated glycosaminoglycan-like polysaccharide from melanin-free ink of the squid <i>Ommastrephes bartrami</i> by negative-ion electrospray tandem mass spectrometry and NMR spectroscopy, GLYCOCONJUGATE JOURNAL, Vol: 25, Pages: 481-492, ISSN: 0282-0080
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- Citations: 56
Boyle M, Richards JS, Chesson JM, et al., 2008, Inhibition of <i>Plasmodium falciparum</i> erythrocyte invasion by heparin-like molecules:: defining molecular and cellular mechanisms., 3rd Molecular Approaches to Malaria Meeting (MAM 2008), Publisher: PERGAMON-ELSEVIER SCIENCE LTD, Pages: S85-S85, ISSN: 0020-7519
Campanero-Rhodes MA, Smith A, Chai W, et al., 2007, <i>N</i>-glycolyl GM1 ganglioside as a receptor for simian virus 40, JOURNAL OF VIROLOGY, Vol: 81, Pages: 12846-12858, ISSN: 0022-538X
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- Citations: 131
Beeson JG, Andrews KT, Boyle M, et al., 2007, Structural basis for binding of <i>Plasmodium falciparum</i> erythrocyte membrane protein 1 to chondroitin sulfate and placental tissue and the influence of protein polymorphisms on binding specificity, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 282, Pages: 22426-22436, ISSN: 0021-9258
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- Citations: 29
Ewers H, Bacia K, Chai W, et al., 2007, The role of membrane lipid composition and tail structure in virus host cell association, entry, and infection., 61st Annual Meeting of the Society-of-General-Physiologists, Publisher: ROCKEFELLER UNIV PRESS, Pages: 3A-3A, ISSN: 0022-1295
Liu Y, Feizi T, Campanero-Rhodes MA, et al., 2007, Neoglycolipid probes prepared via oxime ligation for microarray analysis of oligosaccharide-protein interactions, Chem.Biol., Vol: 14, Pages: 847-859
Neoglycolipid technology is the basis of a microarray platform for assigning oligosaccharide ligands for carbohydrate-binding proteins. The strategy for generating the neoglycolipid probes by reductive amination results in ring opening of the core monosaccharides. This often limits applicability to short-chain saccharides, although the majority of recognition motifs are satisfactorily presented with neoglycolipids of longer oligosaccharides. Here, we describe neoglycolipids prepared by oxime ligation. We provide evidence from NMR studies that a significant proportion of the oxime-linked core monosaccharide is in the ring-closed form, and this form selectively interacts with a carbohydrate-binding protein. By microarray analyses we demonstrate the effective presentation with oxime-linked neoglycolipids of (1) Lewis(x) trisaccharide to antibodies to Lewis(x), (2) sialyllactose analogs to the sialic acid-binding receptors, siglecs, and (3) N-glycans to a plant lectin that requires an intact N-acetylglucosamine core
Matthews S, 2007, Atomic resolution basis for host cell recognition by Toxoplasma gondii
Blumenschein TMA, Friedrich N, Childs RA, et al., 2007, Atomic resolution insight into host cell recognition by <i>Toxoplasma gondii</i>, EMBO JOURNAL, Vol: 26, Pages: 2808-2820, ISSN: 0261-4189
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- Citations: 86
Lv H, Yu G, Sun L, et al., 2007, Elevate level of glycosaminoglycans and altered sulfation pattern of chondroitin sulfate are associated with differentiation status and histological type of human primary hepatic carcinoma, ONCOLOGY, Vol: 72, Pages: 347-356, ISSN: 0030-2414
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- Citations: 41
Yu G, Zhao X, Yang B, et al., 2006, Sequence determination of sulfated carrageenan-derived oligosaccharides by high-sensitivity negative-ion electrospray tandem mass spectrometry, ANALYTICAL CHEMISTRY, Vol: 78, Pages: 8499-8505, ISSN: 0003-2700
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- Citations: 68
Hamza D, Lucas R, Feizi T, et al., 2006, First synthesis of heparan sulfate tetrasaccha rides containing both <i>N</i>-acetylated and <i>N</i>-unsubstituted glucosamine -: Search for putative 10E4 epitopes, CHEMBIOCHEM, Vol: 7, Pages: 1856-1858, ISSN: 1439-4227
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- Citations: 16
Liu Y, Chai W, Crocker PR, et al., 2006, Neoglycolipids prepared via oxime-ligation for microarray analysis of carbohydrate-protein interactions, Meeting of the Society-for-Gylcobiology, Publisher: OXFORD UNIV PRESS INC, Pages: 1140-1140, ISSN: 0959-6658
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- Citations: 2
Beeson JG, Chai W, Andrews KT, et al., 2006, Molecular basis of placental malaria - Structural requirements of chondroitin sulfates for binding PfEMP1, the influence of protein polymorphisms on binding specificity, and identification of optimal inhibitors of parasite adhesion, AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, Vol: 75, Pages: 193-193, ISSN: 0002-9637
Campanero-Rhodes MA, Childs RA, Chai W, et al., 2006, A survey of Siglec binding preferences using carbohydrate microarrays, Meeting of the Society-for-Gylcobiology, Publisher: OXFORD UNIV PRESS INC, Pages: 1140-1140, ISSN: 0959-6658
Zhang ZQ, Yu GL, Zhao X, et al., 2006, Sequence analysis of alginate-derived oligosaccharides by negative-ion electrospray tandem mass spectrometry, JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, Vol: 17, Pages: 621-630, ISSN: 1044-0305
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- Citations: 70
Palma AS, Feizi T, Zhang YB, et al., 2006, Ligands for the β-glucan receptor, Dectin-1, assigned using "designer" microarrays of oligosaccharide probes (neoglycolipids) generated from glucan polysaccharides, JOURNAL OF BIOLOGICAL CHEMISTRY, Vol: 281, Pages: 5771-5779
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- Citations: 281
Chai W, Piskarev VE, Mulloy B, et al., 2006, Analysis of chain and blood group type and branching pattern of sialylated oligosaccharides by negative ion electrospray tandem mass spectrometry, Anal.Chem., Vol: 78, Pages: 1581-1592
We previously reported sequence determination of neutral oligosaccharides by negative ion electrospray tandem mass spectrometry on a quadrupole-orthogonal time-of-flight instrument with high sensitivity and without the need of derivatization. In the present report, we extend our strategies to sialylated oligosaccharides for analysis of chain and blood group types together with branching patterns. A main feature in the negative ion mass spectrometry approach is the unique double glycosidic cleavage induced by 3-glycosidic substitution, producing characteristic D-type fragments which can be used to distinguish the type 1 and type 2 chains, the blood group related Lewis determinants, 3,6-disubstituted core branching patterns, and to assign the structural details of each of the branches. Twenty mono- and disialylated linear and branched oligosaccharides were used for the investigation, and the sensitivity achieved is in the femtomole range. To demonstrate the efficacy of the strategy, we have determined a novel complex disialylated and monofucosylated tridecasaccharide that is based on the lacto-N-decaose core. The structure and sequence assignment was corroborated by methylation analysis and 1H NMR spectroscopy
Chai W, Piskarev VE, Mulloy B, et al., 2006, Analysis of chain and blood group type and branching pattern of sialylated oligosaccharides by negative ion electrospray tandem mass spectrometry, Analytical Chemistry, Vol: 78, Pages: 1581-1592, ISSN: 0003-2700
Liu Y, Chai W, Childs RA, et al., 2006, Preparation of neoglycolipids with ring-closed cores via chemoselective oxime-ligation for microarray analysis of carbohydrate-protein interactions, Methods Enzymol., Vol: 415, Pages: 326-340
Affinities of most oligosaccharide-protein interactions are so low that multivalent forms of ligand and protein are required for detecting interactions. The neoglycolipid (NGL) technology was designed to address the need for microscale presentation of oligosaccharides in a multivalent form for studying carbohydrate-protein interactions, and this is now the basis of a state-of-the-art carbohydrate microarray system. NGL technology involves conjugating oligosaccharides by reductive amination to the aminolipid 1,2-dihexadecyl-sn-glycero-3-phosphoethanolamine (DHPE). Other than ring-opening of the monosaccharide residues at reducing ends, oligosaccharides remain intact, and the NGLs derived from trisaccharides or larger oligosaccharides have performed well for the majority of carbohydrate-recognition systems that have the peripheral or backbone regions of oligosaccharides as recognition motifs. However, ring-opening of reducing end monosaccharides limits applicability to very short oligosaccharides (di- and trisaccharides) and, potentially, to N-glycans recognized by proteins such as Pisum sativum agglutinin (pea lectin) that require both intact core and backbone regions for strong binding. This chapter describes a method for preparing NGLs (designated AO-NGLs) from reducing oligosaccharides by chemoselective oxime-ligation to a new lipid reagent, N-aminooxyacetyl-DHPE. Microarray analyses of the AO-NGL derived from Lewis x (Le(x)) trisaccharide probed with anti-Le(x) antibodies indicate that a significant proportion of the core monosaccharide linked to lipid is in ring-closed form. Thus, AO-NGLs have broadened the applicability of NGLs as probes in studies of carbohydrate-protein interactions
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