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@article{You:2021:j.cnki.11-1802/ts.027472,
author = {You, X and Guo, J and Zhang, H and Yan, J and Chai, W and Zhou, W and Liu, L and Li, Y and Wu, J and Zhan, X},
doi = {j.cnki.11-1802/ts.027472},
journal = {Food and Fermentation Industries},
pages = {8--14},
title = {Synthesis of 3′-sialyllactose by coupling fermentation with different strains},
url = {http://dx.doi.org/10.13995/j.cnki.11-1802/ts.027472},
volume = {47},
year = {2021}
}

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TY  - JOUR
AB  - Sialyllactose has broad market prospects in infant formula industry. However, it is still a challenge to produce sialyllactose in large-scale. Escherichia coli JM109(DE3) / pET28a-neuA and E. coli JM109(DE3) / pET28a-nst were constructed to synthesize cytidine 5′-monophosphate N-acetylneuraminic acid (CMP-Neu5Ac) and 3′-sialyllactose, respectively. And then the optimized condition of CMP-Neu5Ac synthesis by E. coli JM109(DE3) / pET28a-neuA was developed. Finally, the whole-cell coupling fermentation method with beer yeast, E. coli JM109(DE3) / pET28a-neuA and E. coli JM109(DE3) / pET28a-nst was established to achieve 3′-sialyllactose. The CMP-Neu5Ac can be supplied and efficiently regenerated by the method, which will lay a forceful technical foundation for the economical and large-scale production of 3′-sialyllactose.
AU  - You,X
AU  - Guo,J
AU  - Zhang,H
AU  - Yan,J
AU  - Chai,W
AU  - Zhou,W
AU  - Liu,L
AU  - Li,Y
AU  - Wu,J
AU  - Zhan,X
DO  - j.cnki.11-1802/ts.027472
EP  - 14
PY  - 2021///
SN  - 0253-990X
SP  - 8
TI  - Synthesis of 3′-sialyllactose by coupling fermentation with different strains
T2  - Food and Fermentation Industries
UR  - http://dx.doi.org/10.13995/j.cnki.11-1802/ts.027472
VL  - 47
ER  -

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