Imperial College London
Alternate

DrWengangChai

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Senior Research Fellow
 
 
 
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Contact

 

+44 (0)20 7594 2596w.chai

 
 
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Location

 

Burlington DanesHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Liu:2012:10.1007/978-1-61779-373-8_8,
author = {Liu, Y and Childs, RA and Palma, AS and Campanero-Rhodes, MA and Stoll, MS and Chai, W and Feizi, T},
doi = {10.1007/978-1-61779-373-8_8},
journal = {Methods Mol.Biol.},
pages = {117--136},
title = {Neoglycolipid-Based Oligosaccharide Microarray System: Preparation of NGLs and Their Noncovalent Immobilization on Nitrocellulose-Coated Glass Slides for Microarray Analyses},
url = {http://dx.doi.org/10.1007/978-1-61779-373-8_8},
volume = {808},
year = {2012}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Carbohydrate microarrays, since their advent in 2002, are revolutionizing studies of the molecular basis of protein-carbohydrate interactions both in endogenous recognition systems and pathogen-host interactions. We have developed a unique carbohydrate microarray system based on the neoglycolipid (NGL) technology, a well-validated microscale approach for generating lipid-tagged oligosaccharide probes for use in carbohydrate recognition studies. This chapter provides an overview of the principles and key features of the NGL-based oligosaccharide microarrays, and describes in detail the basic techniques - from the preparation of NGL probes to the generation of microarrays using robotic arraying hardware, as well as a general protocol for probing the microarrays with carbohydrate-binding proteins
AU  - Liu,Y
AU  - Childs,RA
AU  - Palma,AS
AU  - Campanero-Rhodes,MA
AU  - Stoll,MS
AU  - Chai,W
AU  - Feizi,T
DO  - 10.1007/978-1-61779-373-8_8
EP  - 136
PY  - 2012///
SP  - 117
TI  - Neoglycolipid-Based Oligosaccharide Microarray System: Preparation of NGLs and Their Noncovalent Immobilization on Nitrocellulose-Coated Glass Slides for Microarray Analyses
T2  - Methods Mol.Biol.
UR  - http://dx.doi.org/10.1007/978-1-61779-373-8_8
UR  - pm:22057521
VL  - 808
ER  -
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