Imperial College London
Alternate

DrWengangChai

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Senior Research Fellow
 
 
 
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Contact

 

+44 (0)20 7594 2596w.chai

 
 
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Location

 

Burlington DanesHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Yan:2018:10.1021/acs.analchem.7b04468,
author = {Yan, J and Ding, J and Jin, G and Yu, D and Yu, L and Guo, Z and Chai, W and Liang, X},
doi = {10.1021/acs.analchem.7b04468},
journal = {Analytical Chemistry},
pages = {3174--3182},
title = {Profiling of sialylated oligosaccharides in mammalian milk using online solid phase extraction-hydrophilic interaction chromatography coupled with negative-ion electrospray mass spectrometry},
url = {http://dx.doi.org/10.1021/acs.analchem.7b04468},
volume = {90},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Sialylated oligosaccharides are important components in mammalian milk. They play a key role in the health and growth of infants by helping to shape up infant’s gastrointestinal microbiota and defense against infection by various pathogenic agents. A detailed knowledge of the structures, compositions, and quantities of the sialylated milk oligosaccharides (SMOs) is a prerequisite for understanding their biological roles. However, because of the presence of very large amounts of lactose and neutral oligosaccharides, accurate analysis of SMOs is difficult. A pretreatment step is required to remove lactose and neutral oligosaccharides but conventional off-line pretreatment methods are time-consuming and of poor reproducibility. In this presentation, we linked solid-phase extraction (SPE) with hydrophilic interaction chromatography (HILIC) followed by mass spectrometry (MS) identification for the analysis of SMOs. A SPE column with electrostatic repulsion function was used for removal of lactose and neutral oligosaccharides, a HILIC analytical column for separation of the SMOs, and negative-ion electrospray ionization tandem MS was used for their identification and sequencing. The success of the established online SPE-HILIC-MS method was demonstrated by profiling of SMOs in human to investigate detailed SMO changes during lactation period and in animals to compare the difference in SMO contents among the different species.
AU  - Yan,J
AU  - Ding,J
AU  - Jin,G
AU  - Yu,D
AU  - Yu,L
AU  - Guo,Z
AU  - Chai,W
AU  - Liang,X
DO  - 10.1021/acs.analchem.7b04468
EP  - 3182
PY  - 2018///
SN  - 0003-2700
SP  - 3174
TI  - Profiling of sialylated oligosaccharides in mammalian milk using online solid phase extraction-hydrophilic interaction chromatography coupled with negative-ion electrospray mass spectrometry
T2  - Analytical Chemistry
UR  - http://dx.doi.org/10.1021/acs.analchem.7b04468
UR  - http://hdl.handle.net/10044/1/56904
VL  - 90
ER  -
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