Imperial College London
Alternate

DrWeiCui

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Senior Lecturer
 
 
 
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Contact

 

+44 (0)20 7594 2124wei.cui Website

 
 
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Location

 

1010Institute of Reproductive and Developmental BiologyHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Li:2019:10.1530/REP-19-0081,
author = {Li, G and Tian, X and Lv, D and Zhang, L and Zhang, Z and Wang, J and Yang, M and Tao, J and Ma, T and Wu, H and Ji, P and Wu, Y and Lian, Z and Cui, W and Liu, G},
doi = {10.1530/REP-19-0081},
journal = {Reproduction},
pages = {415--427},
title = {NLRP7 is expressed in ovine ovary and associated with in vitro preimplantation embryo development},
url = {http://dx.doi.org/10.1530/REP-19-0081},
volume = {158},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - NLRP (NACHT, LRR and PYD domains-containing proteins) family plays the pivotal roles in mammalian reproduction. Mutation of NLRP7 is often associated with human recurrent hydatidiform moles. Few studies regarding the functions of NLRP7 have been performed in another mammalian species rather than humans. In the current study, for the first time, the function of NLRP7 has been explored in ovine ovary. NLRP7 protein was mainly located in ovarian follicles and in the in vitro pre-implantation embryos. To identify its origin, 763bp partial CDS of NLRP7 deriving from sheep cumulus oocyte complexes (COCs) was cloned, it showed a great homology with Homo sapiens. The high levels of mRNA and protein of NLRP7 were steadily expressed in oocytes, parthenogenetic embryos or IVF embryos. NLRP7 knockdown by the combination of siRNA and shRNA jeopardized both the parthenogenetic and IVF embryo development. The results strongly suggest that NLRP7 plays an important role in ovine reproduction. The potential mechanisms of NLRP7 will be fully investigated in the future.
AU  - Li,G
AU  - Tian,X
AU  - Lv,D
AU  - Zhang,L
AU  - Zhang,Z
AU  - Wang,J
AU  - Yang,M
AU  - Tao,J
AU  - Ma,T
AU  - Wu,H
AU  - Ji,P
AU  - Wu,Y
AU  - Lian,Z
AU  - Cui,W
AU  - Liu,G
DO  - 10.1530/REP-19-0081
EP  - 427
PY  - 2019///
SN  - 0022-4251
SP  - 415
TI  - NLRP7 is expressed in ovine ovary and associated with in vitro preimplantation embryo development
T2  - Reproduction
UR  - http://dx.doi.org/10.1530/REP-19-0081
UR  - https://www.ncbi.nlm.nih.gov/pubmed/31505467
UR  - https://rep.bioscientifica.com/view/journals/rep/aop/rep-19-0081.xml
UR  - http://hdl.handle.net/10044/1/73655
VL  - 158
ER  -
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