Imperial College London
Alternate

ProfessorXiao-NingXu

Faculty of Medicine, Department of Infectious Disease

Chair in Human Immunology
 
 
 
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Contact

 

+44 (0)20 3315 6558x.xu

 
 
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Assistant

 

Dr Victoria Male +44 (0)20 3315 3000

 
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Location

 

Chelsea and Westminster HospitalChelsea and Westminster Campus

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Summary

 

Publications

Citation

BibTex format

@article{Chen:2015:10.1097/QAI.0000000000000649,
author = {Chen, Y and Li, N and Zhang, T and Huang, X and Cai, F and Vandergrift, N and Xin, R and Meng, Z and Zhang, X and Jiang, C and Xu, X and Montefiori, DC and Gao, F and Wu, H},
doi = {10.1097/QAI.0000000000000649},
journal = {JAIDS-Journal of Acquired Immune Deficiency Syndromes},
pages = {403--412},
title = {Comprehensive Characterization of the Transmitted/Founder env Genes From a Single MSM Cohort in China},
url = {http://dx.doi.org/10.1097/QAI.0000000000000649},
volume = {69},
year = {2015}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Background: The men having sex with men (MSM) population has become one of the major risk groups for HIV-1 infection in China. However, the epidemiological patterns, function of the env genes, and autologous and heterologous neutralization activity in the same MSM population have not been systematically characterized.Methods: The env gene sequences were obtained by the single genome amplification. The time to the most recent common ancestor was estimated for each genotype using the Bayesian Markov Chain Monte Carlo approach. Coreceptor usage was determined in NP-2 cells. Neutralization was analyzed using Env pseudoviruses in TZM-bl cells.Results: We have obtained 547 full-length env gene sequences by single genome amplification from 30 acute/early HIV-1–infected individuals in the Beijing MSM cohort. Three genotypes (subtype B, CRF01_AE, and CRF07_BC) were identified and 20% of the individuals were infected with multiple transmitted/founder (T/F) viruses. The tight clusters of the MSM sequences regardless of geographic origins indicated nearly exclusive transmission within the MSM population and limited number of introductions. The time to the most recent common ancestor for each genotype was 10–15 years after each was first introduced in China. Disparate preferences for coreceptor usages among 3 genotypes might lead to the changes in percentage of different genotypes in the MSM population over time. The genotype-matched and genotype-mismatched neutralization activity varied among the 3 genotypes.Conclusions: The identification of unique characteristics for transmission, coreceptor usage, neutralization profile, and epidemic patterns of HIV-1 is critical for the better understanding of transmission mechanisms, development of preventive strategies, and evaluation of vaccine efficacy in the MSM population in China.
AU  - Chen,Y
AU  - Li,N
AU  - Zhang,T
AU  - Huang,X
AU  - Cai,F
AU  - Vandergrift,N
AU  - Xin,R
AU  - Meng,Z
AU  - Zhang,X
AU  - Jiang,C
AU  - Xu,X
AU  - Montefiori,DC
AU  - Gao,F
AU  - Wu,H
DO  - 10.1097/QAI.0000000000000649
EP  - 412
PY  - 2015///
SN  - 1944-7884
SP  - 403
TI  - Comprehensive Characterization of the Transmitted/Founder env Genes From a Single MSM Cohort in China
T2  - JAIDS-Journal of Acquired Immune Deficiency Syndromes
UR  - http://dx.doi.org/10.1097/QAI.0000000000000649
UR  - http://hdl.handle.net/10044/1/40106
VL  - 69
ER  -
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