Imperial College London
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ProfessorXiaodongZhang

Faculty of Medicine, Department of Infectious Disease

Professor of Macromolecular Structure and Function
 
 
 
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Contact

 

+44 (0)20 7594 3151xiaodong.zhang Website

 
 
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Assistant

 

Miss Kelly Butler +44 (0)20 7594 2763

 
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Location

 

104Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{von:2016:10.1038/ncomms12813,
author = {von, Nicolai C and Ehlén, Å and Martin, C and Zhang, X and Carreira, A},
doi = {10.1038/ncomms12813},
journal = {Nature Communications},
pages = {12813--12813},
title = {A second DNA binding site in human BRCA2 promotes homologous recombination.},
url = {http://dx.doi.org/10.1038/ncomms12813},
volume = {7},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - BRCA2 tumour-suppressor protein is well known for its role in DNA repair by homologous recombination (HR); assisting the loading of RAD51 recombinase at DNA double-strand breaks. This function is executed by the C-terminal DNA binding domain (CTD) which binds single-stranded (ss)DNA, and the BRC repeats, which bind RAD51 and modulate its assembly onto ssDNA. Paradoxically, analysis of cells resistant to DNA damaging agents missing the CTD restore HR proficiency, suggesting another domain may take over its function. Here, we identify a region in the N terminus of BRCA2 that exhibits DNA binding activity (NTD) and provide evidence for NTD promoting RAD51-mediated HR. A missense variant detected in breast cancer patients located in the NTD impairs HR stimulation on dsDNA/ssDNA junction containing substrates. These findings shed light on the function of the N terminus of BRCA2 and have implications for the evaluation of breast cancer variants.
AU  - von,Nicolai C
AU  - Ehlén,Å
AU  - Martin,C
AU  - Zhang,X
AU  - Carreira,A
DO  - 10.1038/ncomms12813
EP  - 12813
PY  - 2016///
SN  - 2041-1723
SP  - 12813
TI  - A second DNA binding site in human BRCA2 promotes homologous recombination.
T2  - Nature Communications
UR  - http://dx.doi.org/10.1038/ncomms12813
UR  - http://hdl.handle.net/10044/1/40773
VL  - 7
ER  -
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