Imperial College London
Portrait Picture

ProfessorXiaodongZhang

Faculty of Medicine, Department of Infectious Disease

Professor of Macromolecular Structure and Function
 
 
 
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Contact

 

+44 (0)20 7594 3151xiaodong.zhang Website

 
 
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Assistant

 

Miss Kelly Butler +44 (0)20 7594 2763

 
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Location

 

104Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Glyde:2017:10.1016/j.molcel.2017.05.010,
author = {Glyde, R and Ye, F and Darbari, V and Zhang, N and Buck, M and Zhang, X},
doi = {10.1016/j.molcel.2017.05.010},
journal = {Molecular Cell},
pages = {106--116},
title = {Structures of RNA polymerase closed and intermediate complexes revealmechanisms of DNA opening and transcription initiation},
url = {http://dx.doi.org/10.1016/j.molcel.2017.05.010},
volume = {67},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Gene transcription is carried out by RNA polymerases (RNAPs). For transcription to occur, the closed promoter complex (RPc), where DNA is double stranded, must isomerize into an open promoter complex (RPo), where the DNA is melted out into a transcription bubble and the single-stranded template DNA is delivered to the RNAP active site. Using a bacterial RNAP containing the alternative σ54 factor and cryoelectron microscopy, we determined structures of RPc and the activator-bound intermediate complex en route to RPo at 3.8 and 5.8 Å. Our structures show how RNAP-σ54 interacts with promoter DNA to initiate the DNA distortions required for transcription bubble formation, and how the activator interacts with RPc, leading to significant conformational changes in RNAP and σ54 that promote RPo formation. We propose that DNA melting is an active process initiated in RPc and that the RNAP conformations of intermediates are significantly different from that of RPc and RPo.
AU  - Glyde,R
AU  - Ye,F
AU  - Darbari,V
AU  - Zhang,N
AU  - Buck,M
AU  - Zhang,X
DO  - 10.1016/j.molcel.2017.05.010
EP  - 116
PY  - 2017///
SN  - 1097-2765
SP  - 106
TI  - Structures of RNA polymerase closed and intermediate complexes revealmechanisms of DNA opening and transcription initiation
T2  - Molecular Cell
UR  - http://dx.doi.org/10.1016/j.molcel.2017.05.010
UR  - http://hdl.handle.net/10044/1/48452
VL  - 67
ER  -
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