37 results found
Friddin MS, Elani Y, Trantidou T, et al., 2019, New Directions for Artificial Cells Using Prototyped Biosystems, ANALYTICAL CHEMISTRY, Vol: 91, Pages: 4921-4928, ISSN: 0003-2700
Ces O, Elani Y, 2019, Community building in synthetic biology, EXPERIMENTAL BIOLOGY AND MEDICINE, Vol: 244, Pages: 281-282, ISSN: 1535-3702
Friddin MS, Bolognesi G, Salehi-Reyhani A, et al., 2019, Direct manipulation of liquid ordered lipid membrane domains using optical traps, COMMUNICATIONS CHEMISTRY, Vol: 2, ISSN: 2399-3669
Trantidou T, Dekker L, Polizzi K, et al., 2018, Functionalizing cell-mimetic giant vesicles with encapsulated bacterial biosensors, INTERFACE FOCUS, Vol: 8, ISSN: 2042-8898
Trantidou T, Friddin MS, Salehi-Reyhani A, et al., 2018, Droplet microfluidics for the construction of compartmentalised model membranes, LAB ON A CHIP, Vol: 18, Pages: 2488-2509, ISSN: 1473-0197
Karamdad K, Hindley JW, Bolognesi G, et al., 2018, Engineering thermoresponsive phase separated vesicles formed via emulsion phase transfer as a content-release platform, CHEMICAL SCIENCE, Vol: 9, Pages: 4851-4858, ISSN: 2041-6520
Bolognesi G, Friddin MS, Salehi-Reyhani A, et al., 2018, Sculpting and fusing biomimetic vesicle networks using optical tweezers, NATURE COMMUNICATIONS, Vol: 9, ISSN: 2041-1723
Hindley JW, Elani Y, McGilvery CM, et al., 2018, Light-triggered enzymatic reactions in nested vesicle reactors, NATURE COMMUNICATIONS, Vol: 9, ISSN: 2041-1723
Elani Y, Trantidou T, Wylie D, et al., 2018, Constructing vesicle-based artificial cells with embedded living cells as organelle-like modules, SCIENTIFIC REPORTS, Vol: 8, ISSN: 2045-2322
Thomas JM, Friddin MS, Ces O, et al., 2017, Programming membrane permeability using integrated membrane pores and blockers as molecular regulators, CHEMICAL COMMUNICATIONS, Vol: 53, Pages: 12282-12285, ISSN: 1359-7345
Trantidou T, Friddin M, Elani Y, et al., 2017, Engineering Compartmentalized Biomimetic Micro- and Nanocontainers, ACS NANO, Vol: 11, Pages: 6549-6565, ISSN: 1936-0851
Salehi-Reyhani A, Ces O, Elani Y, 2017, Artificial cell mimics as simplified models for the study of cell biology, EXPERIMENTAL BIOLOGY AND MEDICINE, Vol: 242, Pages: 1309-1317, ISSN: 1535-3702
Trantidou T, Elani Y, Parsons E, et al., 2017, Hydrophilic surface modification of PDMS for droplet microfluidics using a simple, quick, and robust method via PVA deposition, MICROSYSTEMS & NANOENGINEERING, Vol: 3, ISSN: 2055-7434
de Bruin A, Friddin MS, Elani Y, et al., 2017, A transparent 3D printed device for assembling droplet hydrogel bilayers (DHBs), RSC ADVANCES, Vol: 7, Pages: 47796-47800, ISSN: 2046-2069
Friddin MS, Bolognesi G, Elani Y, et al., The optical assembly of bilayer networks from cell-sized droplets for synthetic biology, Systems and Synthetic Biology
Elani Y, 2016, Construction of membrane-bound artificial cells using microfluidics: a new frontier in bottom-up synthetic biology., Biochem Soc Trans, Vol: 44, Pages: 723-730
The quest to construct artificial cells from the bottom-up using simple building blocks has received much attention over recent decades and is one of the grand challenges in synthetic biology. Cell mimics that are encapsulated by lipid membranes are a particularly powerful class of artificial cells due to their biocompatibility and the ability to reconstitute biological machinery within them. One of the key obstacles in the field centres on the following: how can membrane-based artificial cells be generated in a controlled way and in high-throughput? In particular, how can they be constructed to have precisely defined parameters including size, biomolecular composition and spatial organization? Microfluidic generation strategies have proved instrumental in addressing these questions. This article will outline some of the major principles underpinning membrane-based artificial cells and their construction using microfluidics, and will detail some recent landmarks that have been achieved.
Friddin MS, Bolognesi G, Elani Y, et al., Optical tweezers to assemble 2D and 3D droplet interface bilayer networks from cell-sized droplets, EMBL Microfluidics
Elani Y, Solvas XC, Edel JB, et al., 2016, Microfluidic generation of encapsulated droplet interface bilayer networks (multisomes) and their use as cell-like reactors., Chemical Communications, Vol: 52, Pages: 5961-5964, ISSN: 1364-548X
Compartmentalised structures based on droplet interface bilayers (DIBs), including multisomes and compartmentalised vesicles, are seen by many as the next generation of biomimetic soft matter devices. Herein, we outline a microfluidic approach for the construction of miniaturised multisomes of pL volumes in high-throughput and demonstrate their potential as vehicles for in situ chemical synthesis.
Friddin MS, Bolognesi G, Elani Y, et al., 2016, Optically assembled droplet interface bilayer (OptiDIB) networks from cell-sized microdroplets, SOFT MATTER, Vol: 12, Pages: 7731-7734, ISSN: 1744-683X
Friddin MS, Bolognesi G, Elani Y, et al., 2016, Light-driven drag and drop assembly of micron-scale bilayer networks for synthetic biology, Pages: 545-546
We have developed a new method to assemble single- or multi-layered networks of droplet interface bilayers (DIBs) from cell-sized droplets using a single beam optical trap (optical tweezers). The novelty of our approach is the ability to directly trap the microdroplets with the laser and manipulate them in 3D to construct DIB networks of user-defined architectures. Our method does not require a complex optical setup, is versatile, contactless, benefits from both high spatial and temporal resolution, and could set a new paradigm for the assembly of smart, synthetic biosystems.
Trantidou T, Elani Y, Ces O, 2016, Versatile strategies for the microfluidic generation of lipid-stabilised double emulsions, Pages: 1595-1596
Lipid-stabilised double emulsions have recently gained much importance in translational healthcare as potential micro-bioreactors for the synthesis of high-end materials, in situ drug delivery, and as templates for artificial cells in synthetic biology. Whilst microfluidic generation of surfactantstabilised systems is well established, lipid-stabilised systems are notoriously more cumbersome to produce, since they require specific surface chemistries and many surface modification techniques are incompatible with lipids. This paper reports a simple, robust and versatile method for the microfluidic generation of stable and monodisperse double emulsions using biologically relevant phospholipids.
Trantidou T, Elani Y, Ces O, 2016, Microfluidic generation of double emulsions as multiphase compartmentalised cell-like systems
Ces O, Elani Y, Karamdad K, et al., 2016, Novel microfluidic technologies for the bottom-up construction of artificial cells
© 2016 Institution of Engineering and Technology. All rights reserved. This talk will outline novel microfluidic strategies for biomembrane engineering that are capable of fabricating vesicles , droplet interface bilayer networks , multisomes  and artificial tissues  where parameters such as membrane asymmetry, membrane curvature, compartment connectivity and individual compartment contents can be controlled. Various bulk methods, such as extrusion, gentle hydration and electroformation, have been synonymous with the formation of lipid vesicles over recent years. However these strategies suffer from significant shortcomings associated with these processes including limited control of vesicle structural parameters such as size, lamellarity, membrane composition and internal contents. To address this technological bottleneck we have developed novel microfluidic platforms to form lipid vesicles in high-Throughput with full control over the composition of both the inner and outer leaflet of the membrane thereby enabling the manufacture of symmetric and asymmetric vesicles. This is achieved by manufacturing microfluidic channels with a step junction, produced by double-layer photolithography, which facilitates the transfer of a W/O emulsion across an oil-water phase boundary and the self-Assembly of a phospholipid bilayer. These platforms are being used to explore the role of asymmetry in biological systems  and study the engineering rules that regulate membrane mediated protein-protein interactions . In addition, these technologies are enabling the construction of biological machines capable of acting as micro-reactors , environmental sensors and smart delivery vehicles  as well as complex multi-compartment artificial cells where the contents and connectivity of each compartment can be controlled. These compartments are separated by biological functional membranes that can facilitate transport between the compartments themselves and between
Carreras P, Elani Y, Law RV, et al., 2015, A microfluidic platform for size-dependent generation of droplet interface bilayer networks on rails, BIOMICROFLUIDICS, Vol: 9, ISSN: 1932-1058
Elani Y, Law RV, Ces O, 2015, Vesicle-based artificial cells: recent developments and prospects for drug delivery, THERAPEUTIC DELIVERY, Vol: 6, Pages: 541-543, ISSN: 2041-5990
Elani Y, 2015, Development of Microfluidic Technologies for the Construction of Multi-Compartment Vesicles and their Applications as Artificial Cells
Elani Y, Law R, Ces O, Vesicle-based artificial cells: recent developments and prospects for drug delivery, Therapeutic delivery, ISSN: 2041-6008
Elani Y, Law RV, Ces O, 2015, Protein synthesis in artificial cells: using compartmentalisation for spatial organisation in vesicle bioreactors, PHYSICAL CHEMISTRY CHEMICAL PHYSICS, Vol: 17, Pages: 15534-15537, ISSN: 1463-9076
Elani Y, Purushothaman S, Booth PJ, et al., 2015, Measurements of the effect of membrane asymmetry on the mechanical properties of lipid bilayers, CHEMICAL COMMUNICATIONS, Vol: 51, Pages: 6976-6979, ISSN: 1359-7345
Elani Y, Law RV, Ces O, 2015, Erratum: Vesicle-based artificial cells: Recent developments and prospects for drug delivery (Therapeutic Delivery (2015) 6: 5 (541-543)), Therapeutic Delivery, Vol: 6, ISSN: 2041-5990
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