Funded by a BBSRC grant under the Tools and Resources Development Fund, we have developed a novel time-resolved automated fluorometer that utilises a fibre-laser-pumped supercontinuum source for versatile excitation and time-correlated single photon counting (TCSPC) for fluorescence lifetime measurements.  The instrument is also equiped with polarisers and a spectrograph or motorised spectrometer to permit conveneint and automated fluorescence measurements resolved with repect to excitationand emission wavelength, fluorescence lifetime and polarisation in a single measurement. 

 

Laser1 The development of this instrument was described in Manning et al, J. Biophoton. 1 (2008) 494–505, doi.org/10.1002/jbio.200810051