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@article{Wright:2021:2515-7647/ac29a2,
author = {Wright, T and Sparks, H and Paterson, C and Dunsby, C},
doi = {2515-7647/ac29a2},
journal = {Journal of Physics: Photonics},
pages = {1--14},
title = {Video-rate remote refocusing through continuous oscillation of a membrane deformable mirror},
url = {http://dx.doi.org/10.1088/2515-7647/ac29a2},
volume = {3},
year = {2021}
}
TY - JOUR
AB - This paper presents the use of a deformable mirror (DM) configured to rapidly refocus a microscope employing a highnumerical aperture objective lens. An Alpao DM97-15 membrane DM was used to refocus a 40×/0.80 NA water-immersionobjective through a defocus range of -50 to 50 m at 26.3 sweeps per second. We achieved imaging with a mean Strehlmetric of > 0.6 over a field of view in the sample of 200×200 m2 over a defocus range of 77 m. We describe anoptimisation procedure where the mirror is swept continuously in order to avoid known problems of hysteresis associatedwith the membrane DM employed. This work demonstrates that a DM-based refocusing system could in the future be used inlight-sheet fluorescence microscopes to achieve video-rate volumetric imaging.
AU - Wright,T
AU - Sparks,H
AU - Paterson,C
AU - Dunsby,C
DO - 2515-7647/ac29a2
EP - 14
PY - 2021///
SN - 2515-7647
SP - 1
TI - Video-rate remote refocusing through continuous oscillation of a membrane deformable mirror
T2 - Journal of Physics: Photonics
UR - http://dx.doi.org/10.1088/2515-7647/ac29a2
UR - https://iopscience.iop.org/article/10.1088/2515-7647/ac29a2
UR - http://hdl.handle.net/10044/1/91558
VL - 3
ER -