Calnexin family - sequence alignment

Regions of secondary and tertiary structure are indicated above the alignment. The signal sequence is highlighted in yellow, the N- and C-terminal portions of the globular domain in light blue, the arm domain (P domain) in pink and the transmembrane and cytoplasmic regions in light green. Transmembrane domains and ER retention signals are highlighted in red. Within the arm domain, the borders between sequence repeats are indicated (|). Repeats are marked 1, for repeat type 1, and 2, for repeat type 2. Pairs of repeats which interact with one another in the calnexin structure are denoted a-d. Beta strands are highlighted in blue and the alpha helix in red, to correspond with the structure of calnexin, left. Residues which are identical in most proteins of the calnexin/calreticulin family are highlighted in green. Residues which are similar are highlighted in cyan. Cysteine residues involved in disulphide bonding in calnexin, and the conserved equivalents in other proteins, are highlighted in yellow. In calnexin, this disulphide bond is essential for ligand binding.

Two acidic side chains which contribute to Ca2+ coordination in the structure of calnexin, and the conserved equivalents in other proteins, are highlighted in purple. Residues which may contribute backbone oxygen atoms to Ca2+ coordination, and the conserved equivalents in other proteins, are highlighted in violet. Highlighted in magenta are residues in calnexin shown to be essential for glucose binding, and the equivalents (identical and similar) in other calnexins and calmegin. Highlighted in orange are equivalent residues in calreticulin that have been shown to be essential for oligosaccharide binding. Highlighted in pale orange are equivalent residues in calreticulin that are less important for oligosaccharide binding than in calnexin.

Accession numbers

Abbreviations: HS = human DM = Drosophila CE = C elegans SC = S cerevisiae SP = S pombe AT = Arabidopsis thaliana

Calnexin HS P27824 DM CG11958 O02393 CG9906 Q9VXF6 also CG1924 Q9I7S9 CE P34652 SC P27825 SP P36581 AT 1 P29402 also 2 Q38798

Calmegin HS O14967

Calreticulin: Human P27797 CE P27798 DM P29413 also AT 1 O04151 2 Q38858 3 O04153

Calreticulin 2 HS Q96L12

If alignments appear scrambled please maximize the width of your browser window.

Structure of luminal domain of canine calnexin

The Ca2+ ion is shown in dark blue. The transmembrane region follows the alpha-helix at the bottom of the structure. Protein Data Bank structure ID: 1JHN.

Signal sequence N domain

HS calnexin MEGKW-----LLCMLLVLGTAIVEAHDGHDDDVIDIEDDLDDVIEEVEDSKPDTTAPPSSPKVTYKAPVPTG--EVYFADSFDRGTLSG-----WILSKAKKDDTDDEIAKYDGKWEVEE

DM calnexin 1 MAWKMGGNRAATLALLFASSLLLLSSANAADLDTESDD-FED--GYVEDVQEEPAVIGGDEKLAYESPVIDAK-KFHFADHFDDVEESRKR---WVLSQAKKDDIAEEISKYDGIWNWES

DM calnexin 2 MAWKM----------LFAI-LLLLSAAKTGYLAPESE-------NSVEDVQIEPGAIYGDEKFAYKSPVIDAE-KFYFADHFDDVEASRKR---WVLSQAKKNDIADEIAKYDGIWNWES

CE calnexin MVNRKWMY-------IFIQ-FLLVSSIRSDDDVFEDDE---------EEVTKGSDDKEEFVPSLFVAPKLSDKSTPNFFDYFPVGSKIGLT---WIKSLAKKDDVDSDIAKYNGEWSIGA

AT calnexin MRQRQ----------LFSV-FLLLLAFVSFQKLCYCDDQTVLY------------------------------------ESFDEPFDGR-----WIVSKNSD---------YEGVWKHAK

SC calnexin MKFSAY---------LWWL-FLN-LALVKGTS------------------------------LLSNVTLAEDS----FWEHFQAYTNTKHLNQEWITSEAVN---NEGSKIYGAQWRLSQ

SP calnexin MKYGK----------VSFL-ALLCSLYVRGSLADPESEQEP---------------------LVFNPTEVKAP----LVEQFQGAWSER-----WIPSHAKRFVNGIEEMSYVGEWTVEE

HS calmegin MHFQA----------FWLC-LGLLFISINAEFMDDDVETEDFEENSEE---IDVNESELSSEIKYKTPQPIG--EVYFAETFDSGRLAG-----WVLSKAKKDDMDEEISIYDGRWEIEE

HS calreticulin MLLSVPLLLGLLGLAVAEPAVYFKEQFLDGDGWTSR---WIESKHKS-DFGKFVLSSGKFYGDEE

CE calreticulin MKSLC-LLAIVAVVSAE--VYFKEEF-NDASWEKR---WVQSKHKD-DFGAFKLSAGKFFDVES

DM calreticulin MMWCKTVIVLLATVGFISAE--VYLKENF-DNENWEDT---WIYSKHPGKEFGKFVLTPGTFYNDAE

HS calreticulin 2 MARALVQLWAICMLRVALATVYFQEEFLDGEHWRNR---WLQSTNDS-RFGHFRLSSGKFYGHKE

N domain

HS calnexin MKES-KLPGDKGLVLMSRAKHHAISAKLNKPFLFDT-KPLIVQYEVNFQNGIECGGAYVKLLSK-TPELNLDQFHDKTP-YTIMFGPDKCGE-DYKLHFIFRHKNPKTGIYEEKHAK-RP

DM calnexin PQRI-VWANDLGLVLKSKAKHAAIAAPLRKPFEFKSDKPLVVQYEVTLQEGQECGGSYLKLLSAGKDTEQLKAFNDKTP-YTIMFGPDKCGN-DVKMHFIFRHVNPINGTITEKHCN-KP

DM calnexin PQRI-FWANDLGLVLKSKAKHAAIAAPFRQPFDFKSNKPLVVQYELTLQEGQDCGGSYLKLLSAGKGTEQLNRFNDKTP-YTIMFGPDKCGN-NLKMHFIFRHVNPLNGNITEKHCK-KP

CE calnexin PTKV-SIEGDLGLIVKTKARHHAIAAKLNTPFAFDA-NTFVVQYDIKFEEGQECGGGYLKLLSEG-AEKDLANFQDKTA-YTIMFGPDKCGA-TGKVHLIFRYKNPINGTISEYHAN-QP

AT calnexin SEG----HEDYGLLVSEKARKYGIVKELDEPLNLKE-GTVVLQYEVRFQEGLECGGAYLKYLRPQEAGWTPQGFDSESP-YSIMFGPDKCGG-TNKVHFILKHKNPKSGEYVEHHLK-FP

SC calnexin G-RLQGSAWDKGIAVRTGNAAAMIGHLLETPINVSETDTLVVQYEIKLDNSLTCGGAFIKLMSGFMNVEALKHYAPDTEGVELVFGPDYCAPEINGVQFAINKVDKITHESKLRYLQEMP

SP calnexin SSGPGALKGEAGLVMKDEAAHHAISYEFDEPINEPE-KDLVVQYEVNPEEGLNCGGAYLKLLAEP--THGEMSN--SID-YRIMFGPDKCGV-NDRVHFIFKHKNPLTGEYSEKHLDSRP

HS calmegin LKEN-QVPGDRGLVLKSRAKHHAISAVLAKPFIFAD-KPLIVQYEVNFQDGIDCGGAYIKLLAD-TDDLILENFYDKTS-YIIMFGPDKCGE-DYKLHFIFRHKHPKTGVFEEKHAK-PP

HS calreticulin --------KDKGLQTSQDARFYALSASFEP-FSNKG-QTLVVQFTVKHEQNIDCGGGYVKLFPN---SLDQTDMHGDSE-YNIMFGPDICGPGTKKVHVIFNYK----G---KNVLI-NK

CE calreticulin --------RDQGIQTSQDAKFYSRAAKFDKDFSNKG-KTLVIQYTVKHEQGIDCGGGYVKVMRA---DADLGDFHGETP-YNVMFGPDICGP-TRRVHVILNYK----G---ENKLI-KK

DM calreticulin --------ADKGIQTSQDARFYAASRKFDG-FSNED-KPLVVQFSVKHEQNIDCGGGYVKLFDC---SLDQTDMHGESP-YEIMFGPDICGPGTKKVHVIFSYK----G---KNHLI-SK

HS calreticulin 2 --------KDKGLQTTQNGRFYAISARFKP-FSNKG-KTLVIQYTVKHEQKMDCGGGYIKVFPA---DIDQKNLNGKSQ-YYIMFGPDICGFDIKKVHVILHFK--------NKYHENKK

N domain P domain |1a |1b |1c |1d

HS calnexin DADLKTYFTDKKTHLYTLILNP-DNSFEILVDQSVVNSGNLL-----NDMTPPVNPSREIEDPEDRKPEDWDERPKIPDPEAVKPDDWDEDAPAKIPDEEATKPEGWLDDEPEYVPDPDA

DM calnexin KNRLEEPFKDKLPHLYQLVVRP-DNSFEIRVDHKIINEGSLL-----TDFKPPVNPPAEIDDPNDHKPESWDEREKIPDPTAHKPEDWDEDAPPQLPDTDAVMPNGWLEDEPDMIFDPTA

DM calnexin NARLGVPFTDKLPHLYQLVVRP-DNSFEIRLDHKIIKEGSLL-----TDFVPPVNPPAEIDDPNDHKPESWDERKKIPDPNAHKPEDWDEDAPPHLPDTDAVMPNGWLEDEPDMIFDPTA

CE calnexin TTIGSTYWDDHNTHLFTLVVKP-TGEYSVSVDGKSLYYGNMM-----SDVTPALTPPKQIFDETDLKPVDWDERENIEDESAVKPDDWDENEPQSVVDEAATKPYDWNEEENELIADPEA

AT calnexin PSVPY----DKLSHVYTAILKP-DNEVRILVDGEEKKKANLLSG---EDFEPALIPAKTIPDPEDKKPEDWDERAKIPDPNAVKPEDWDEDAPMEIEDEEAEKPEGWLDDEPEEVDDPEA

SC calenexin LSKLT---DTSQSHLYTLIIDESAQSFQILIDGKTVMVREHIEDKKKVNFEPPITPPLMIPDVSVAKPHDWDDRIRIPDPEAVKLSDRDERDPLMIPHPDGTEPPEWNSSIPEYILDPNA

SP calnexin ASLLK----PGITNLYTLIVKP-DQTFEVRINGDVVRQGSLF-----YDFIPPVLPPVEIYDPEDIKPADWVDEPEIPDPNAVKPDDWDEDAPRMIPDPDAVKPEDWLEDEPLYIPDPEA

HS calmegin DVDLKKFFTDRKTHLYTLVMNP-DDTFEVLVDQTVVNKGSLL-----EDVVPPIKPPKEIEDPNDKKPEEWDERAKIPDPSAVKPEDWDESEPAQIEDSSVVKPAGWLDDEPKFIPDPNA

HS calreticulin DIRCK---DDEFTHLYTLIVRP-DNTYEVKIDNSQVESGSLE-----DDWD--FLPPK-----------------KIKDPDASKPEDWDERA--KIDDPTDSKPEDWDKPE--HIPDPDA

CE calreticulin EITCK---SDELTHLYTLILNS-DNTYEVKIDGESAQTGSLE-----EDWD--LLPAK-----------------KIKDPDAKKPEDWDERE--YIDDAEDAKPEDWEKPE--HIPDPDA

DM calreticulin DIRCK---DDVYTHFYTLIVRP-DNTYEVLIDNEKVESGNLE-----DDWD--FLAPK-----------------KIKDPTATKPEDWDDRA--TIPDPDDKKPEDWDKPE--HIPDPDA

HS calreticulin 2 LIRCK---VDGFTHLYTLILRP-DLSYDVKIDGQSIESGSIE-----YDWN--LTSLK-----------------KETSPAESK--DWEQ--------TKDNKAQDWEK----HFLDAST

1d |2d |2c |2b |2a |C domain

HS calnexin EKPEDWDEDMDGEWEAPQIANPRCESAPGCGVWQRPVIDNPNYKGKWKPPMIDNPSYQGIWKPRKIPNPDFFEDLEPFR-MTPFSAIGLELWSMTSDIFFDNFIICADRRIVDDWANDGW

DM calnexin TKPEDWDAEIDGEWEAPLVDNPVCEKAPGCGKWKAPLIPNPNYKGKWRAPMIENPNYQGKWAPRKIPNPDFFEDLKPFQ-MTPISAVGLELWSMSSDILFDNLIITDDVEVARDFAANSF

DM calnexin IEPEDWNSEIDGEWEAPLVENPVCKKAPGCGKWKAPLIPNPNYKGKWSAPMIENPNNQGKWAPRKIANPDFFEDLKPFQ-MTPINAVGLELWSMSSDILFDNLIITDDVELARDFAANSF

CE calnexin KKPQDWDEDMDGSWEAPLIDNPACKGLSGCGTWKAPTIKNPKYKGKWIRPKISNPAFKGKWTARLIDNPNYFE-PKPFAGLAPITAVGIEMWTMSENILFDNILITSSEEDSSDVAKQTF

AT calnexin TKPEDWDDEEDGMWEAPKIDNPKCEAAPGCGEWKRPMKRNPAYKGKWSSPLIDNPAYKGIWKPRDIPNPDYFELDRPDY--EPIAAIGIEIWTMQDGILFDNILIAKDEKVAETYRQTTW

SC calnexin QKPSWWKELEHGEWIPPMIKNPLCTAERGCGQQIPGLINNAKYKGPGELNEIINPNYMGEWHPPEIENPLYYEEQHPLRIENVISGVILEFWSGSPNMLISNIYVGKNVTEAQIIGNKTW

SP calnexin QKPEDWDDEEDGDWIPSEIINPKCIEGAGCGEWKPPMIRNPNYRGPWSPPMIPNPEFIGEWYPRKIPNPDYFDDDHPSH-FGPLYGVGFELWTMQPNIRFSNIYVGHSIEDAERLGNETF

HS calmegin EKPDDWNEDTDGEWEAPQILNPACR--IGCGEWKPPMIDNPKYKGVWRPPLVDNPNYQGIWSPRKIPNPDYFEDDHPFL-LTSFSALGLELWSMTSDIYFDNFIICSEKEVADHWAADGW

HS calreticulin KKPEDWDEEMDGEWEPPVIQNPEYK-----GEWKPRQIDNPDYKGTWIHPEIDNPEY--------------SPDPSIYA-YDNFGVLGLDLWQVKSGTIFDNFLITNDEAYAEEFGNETW

CE calreticulin KKPEDWDDEMDGEWEPPMIDNPEYK-----GEWKPKQIKNPAYKGKWIHPEIENPEY--------------TPDDELYS-YESWGAIGFDLWQVKSGTIFDNIIITDSVEEAEAHAAETF

DM calreticulin TKPEDWDDEMDGEWEPPMIDNPEFK-----GEWQPKQLDNPNYKGAWEHPEIANPEY--------------VPDDKLYL-RKEICTLGFDLWQVKSGTIFDNVLITDDVELAAKAAAEVK

HS calreticulin 2 SKQSDWNGDLDGDWPAPMLQKPPYQ-----DGLKPEGIH----KDVWLHRKMKNTDY--------------LTQYDLSE-FENIGAIGLELWQVRSGTIFDNFLITDDEEYADNFGKATW

C domain Transmembrane Transmembrane

HS calnexin GL------KKAADGAAEPGVVGQMIEAAEERPWLWVVYILTVALPVFLVILFCCS--GKK-----QTSGMEYKKTDAPQPDVK-----EEEEEKEE----EKDKGDEEEEGE---EKLEE

DM calnexin DI------KRRYIDRESDSFVNKVVELAKANPSIWGIGLVAIVALVALTIYCRFGTAKSQDSAA-KKAAAEAKKSDDPQPDDE---PEAEEESDER---AAGDTSKESTPLSASPKKNQK

DM calnexin DI------KRLYIDRE--SFGNKVVELARANRAIWGIVIVAIAVPVAITIFCKFGLGPSRVAAAKKAAAAAAKKTDDPQPDDD---LGAEEETDER---AAGDTNKESTPLSASPKKNQK

CE calnexin YVKQKEEYRLAAATGNGNGFFQQIIDATNEKPWLWAVYILCVLLPLVAIGVFCFG-KQSK------PTPNFAKKSDAYSADDDRVPNLVDDDEEEIIGDEEDDVNQPGPSGSQSNPEPQD

AT calnexin KP------KFDVEKEKQ--KAEEEAAGSADGLKSYQKVVFDLLNKVADLSFLS--AYKSK-------ITELIEKAEQ-QPNLTIGVLVAIVVVFFSLFLKLIFGGKKAAAPVEK-------

SC calnexin LMR-----DRAFRGSDGP-TERKFMNSRLGNLQTTFHNERESPNPFDRIIDRILEQPLK-------------------------FVLTAAVVLLTTSVLCCVVFT

SP calnexin LP------KLKAERELLS--KQESMEKQSMHVDEESNQILEKFLDVYDIIKAKLPPNVAE-------KVDYYVETIIETPEIGIAIVAVLGSLTAVILTCYFYFFASSSPASLSTGTTEA

HS calmegin RW------KIMIANANKPGVLKQLMAAAEGHPWLWLIYLVTAGVPIALITSFCWPRKVKK-----KHKDTEYKKTDICIPQTK---GVLEQEEKEEKAALEKPMDLEEEKKQNDGEMLEK

HS calreticulin GV------TKAAEKQMK--DKQDEEQRL-------------------------------K-------EEEEDKKR----K---------EEEEAEDK---EDDEDKDEDEED----E-ED

CE calreticulin DK------LKTVEKEKK--EKADEETR--------------------------------K-------AEEEARKK----A---------EEE-KEAK---KDDDEEEKEEE---------

DM calreticulin N-------TQAGEKKMK--EAQDEVQRK-------------------------------K-------DEEEAKKA----S---------DKD-DED----EDDDDEEKDDES----K-QD

HS calreticulin 2 GE------TKGPEREMD--AIQAKEEMK-------------------------------K----------------------------AREEEEEELLSGKI------------------

ER Retention Signal

HS calnexin KQKSDAEEDGG----TVS-QEEEDRKPK-------AEEDEILN------RSPRNRKPRRE

DM calnexin SDLDDNEEESK----AAESREPAQTEES--------------N------TKTRKRQARKE

DM calnexin SYLDNNEDEED----TLKNKEPTPKKRK--------------R------------KALKE

CE calnexin EEENAEQQSANSS--QSSAAEEEDDEHVVPENEPVKPTEEFAKKSPKNTGGAKRRTARRGD

AT calnexin KKPEVAESSKS--------GDEAEKKEE--------------------TAAPRKRQPRRDN

SP calnexin EK-EQQEK--------FKQETETEKIDVS------------------YAPETESPTAKNED

HS calmegin EEESEPEEKSEEEIEIIEGQEESNQSNKSGSEDEMKEADESTGSGDGPIKSVRKRRVRKD

HS calreticulin KEEDEEEDVPG-----Q-AKDEL

CE calreticulin ------E-----------GHDEL

DM calreticulin K--DQSE------------HDEL

HS calreticulin 2 NRHEHYFNQFH-------RRNEL

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This page last updated:	Tuesday, 19 September 2006	Animal lectins home
Contact information:	This site is supported by:
Kurt Drickamer Division of Molecular Biosciences Faculty of Natural Sciences Imperial College London Email: k.drickamer@imperial.ac.uk