TY - JOUR AB - The predictability and robustness of engineered bacteria depend on the many interactions between synthetic constructs and their host cells. Expression from synthetic constructs is an unnatural load for the host that typically reduces growth, triggers stresses and leads to decrease in performance or failure of engineered cells. Work in systems and synthetic biology has now begun to address this through new tools, methods and strategies that characterise and exploit host-construct interactions in bacteria. Focusing on work in E. coli, we review here a selection of the recent developments in this area, highlighting the emerging issues and describing the new solutions that are now making the synthetic biology community consider the cell just as much as they consider the construct. AU - Borkowski,O AU - Ceroni,F AU - Stan,GB AU - Ellis,T DO - 10.1016/j.mib.2016.07.009 EP - 130 PY - 2016/// SN - 1879-0364 SP - 123 TI - Overloaded and stressed: whole-cell considerations for bacterial synthetic biology. T2 - Current Opinion in Microbiology UR - http://dx.doi.org/10.1016/j.mib.2016.07.009 UR - http://hdl.handle.net/10044/1/54638 VL - 33 ER -