TY - JOUR AB - Softwood is an abundantly available feedstock for the bio-based industry, however, achieving cost-effective sugar release is particularly challenging owing to its guaiacyl-only lignin. Here, we report the highly effective pretreatment of the softwood pine (Pinus sylvestris) using ionoSolv pretreatment, a novel ionic liquid-based lignocellulose fractionation technology. Three protic, low-cost ionic liquids, 1-butylimidazolium hydrogen sulfate, triethylammonium hydrogen sulfate and N,N-dimethylbutylammonium hydrogen sulfate, were used to fractionate the biomass into a carbohydrate-rich pulp and a lignin. The carbohydrate-rich pulp was hydrolysed into fermentable sugars by enzymatic saccharification. Under the most successful pretreatment conditions, quantitative glucose release from the pulp was achieved, which equates to a projected glucose release of 464 mg per gram of pine wood entering the process. We further intensified the process by increasing the solid to solvent ratio up to 1:2 g/g while maintaining saccharification yields of 75% of the theoretical maximum. We also demonstrate for the first time that N,N-dimethylbutylammonium hydrogen sulfate, [DMBA][HSO4] is an excellent low-cost pretreatment solvent, surpassing the pretreatment effectiveness of its symmetrically substituted analogue triethylammonium hydrogen sulfate. This shows that ionoSolv pretreatment with protic hydrogen sulfate ionic liquids is a truly feedstock-independent pretreatment option, further increasing the commercial potential of this pretreatment technology. AU - Gschwend,F AU - Chambon,C AU - Biedka,M AU - Brandt-Talbot,A AU - Fennell,P AU - Hallett,J DO - 10.1039/C8GC02155D EP - 703 PY - 2019/// SN - 1463-9262 SP - 692 TI - Quantitative glucose release from softwood after pretreatment with low-cost ionic liquids T2 - Green Chemistry UR - http://dx.doi.org/10.1039/C8GC02155D UR - http://hdl.handle.net/10044/1/65628 VL - 21 ER -