TY - JOUR AB - Matrix metalloproteinases (MMPs) contribute to the breakdown of tissue structures such as the basement membrane, promoting tissue fibrosis. Here we developed an electrospun membrane biofunctionalized with a fragment of the laminin β1-chain to modulate the expression of MMP2 in this context. We demonstrate that interfacing of the β1-fragment with the mesothelium of the peritoneal membrane via a biomaterial abrogates the release of active MMP2 in response to transforming growth factor β1 and rescues tissue integrity ex vivo and in vivo in a mouse model of peritoneal fibrosis. Importantly, our data demonstrate that the membrane inhibits MMP2 expression. Changes in the expression of epithelial-to-mesenchymal transition (EMT)-related molecules further point towards a contribution of the modulation of EMT. Biomaterial-based presentation of regulatory basement membrane signals directly addresses limitations of current therapeutic approaches by enabling a localized and specific method to counteract MMP2 release applicable to a broad range of therapeutic targets. AU - Horejs,CM AU - St-Pierre,JP AU - Ojala,JRM AU - Steele,JAM AU - Barros,da Silva P AU - Rynne-Vidal,A AU - Maynard,SA AU - Hansel,CS AU - Rodriguez-Fernandez,C AU - Mazo,MM AU - You,AYF AU - Wang,AJ AU - von,Erlach T AU - Tryggvason,K AU - Lopez-Cabrera,M AU - Stevens,MM DO - 10.1038/ncomms15509 PY - 2017/// SN - 2041-1723 TI - Preventing tissue fibrosis by local biomaterials interfacing of specific cryptic extracellular matrix information T2 - Nature Communications UR - http://dx.doi.org/10.1038/ncomms15509 UR - http://hdl.handle.net/10044/1/49855 VL - 8 ER -